Expression of<i>Serratia marcescens</i>Chitinase Gene in<i>Rhizobium meliloti</i>During Symbiosis on Alfalfa Roots

Sitrit, Yaron; Barak, Ze’ev; Kapulnik, Yoram; Oppenheim, Amos B.; Chet, I.

doi:10.1094/mpmi-6-293

Cited by 30 publications

(6 citation statements)

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“…This approach has been found to be successful when foreign chitinases are inserted into symbiotic endophytic bacteria living inside the plant. This approach was successfully used by Sitrit et al [139] who introduced the ChiA gene that codes for an extracellular chitinase from Serratia marcescens into the nitrogen-fixing bacterium Sinorhizobium meliloti (syn. Rhizobium melliloti).…”

Section: The Role Of Chitinases In Plant Defensementioning

confidence: 99%

A Review of the Applications of Chitin and Its Derivatives in Agriculture to Modify Plant-Microbial Interactions and Improve Crop Yields

2013

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Abstract:In recent decades, a greater knowledge of chitin chemistry, and the increased availability of chitin-containing waste materials from the seafood industry, have led to the testing and development of chitin-containing products for a wide variety of applications in the agriculture industry. A number of modes of action have been proposed for how chitin and its derivatives can improve crop yield. In addition to direct effects on plant nutrition and plant growth stimulation, chitin-derived products have also been shown to be toxic to plant pests and pathogens, induce plant defenses and stimulate the growth and activity of beneficial microbes. A repeating theme of the published studies is that chitin-based treatments augment and amplify the action of beneficial chitinolytic microbes. This article reviews the evidence for claims that chitin-based products can improve crop yields and the current understanding of the modes of action with a focus on plant-microbe interactions.

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Section: The Role Of Chitinases In Plant Defensementioning

confidence: 99%

A Review of the Applications of Chitin and Its Derivatives in Agriculture to Modify Plant-Microbial Interactions and Improve Crop Yields

2013

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“…Furthermore, expression of the chiA gene from S. marcescens in R. meliloti demonstrated that the nodule extracts from chiA expressing alfalfa plants caused lysis of Rhizoctonia solani hyphal tips (Minic et al 1998). Moreover, Rhizobium meliloti expressed Serratia marcescens chitinase gene efficiently degrades hyphal tips of Rhizoctonia solani (Sitrit et al 1993). Rhizobial species harbours plasmids that vary in size and number.…”

Section: Introductionmentioning

confidence: 99%

Recombinants Rhizobacteria Enhanced Biological Control of Rhizoctonia solani infected Faba Bean

Kamal

Zaied

El–Hadi

et al. 2020

Journal of Agricultural Chemistry and Biotechnology

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This study aimed to test the effect of Rhizobium recombinants on the vegetative growth and yield components of faba bean under biotic stress of Rhizoctonia solani. Tweleve transconjugants resulted from six crosses were evaluated for their antigonism against Rhizoctonia solani. The results showed variations in chitin hydrolysis after genome shortening. At the molecular level, the recipient strains of rhizobia showed more similarity with each other. However, the donar strains of Serratia and pseudomonas showed less similarity. Meanwhile , Rhizobium transconjugants genomes have a similar distribution of bands. Both transconjugants Tr 4 and Tr15 showed more genome size than the other ones due to high level of plasmid transfer. The same transconjugants recorded higher values of chitin hydrolysis zone due to higher expression of chitinase genes. Most transconjugants showed significant increase in nodulation above the uninoculated plants grown in the infected soil. Rhizobium inoculants improved the yield , chlorophyll concentration and NPK content of the plants grown in the soil infected with Rhizoctonia solani compared with uninoculated plants. The results indicated that Rhizobium inoculants improved the growth and yield of plants grown under biotic stress condition. In addition, improved rhizobia with chitinolytic enzymes producing genes could inhibit significantly the growth of pathogenic fungi.

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“…Recent developments based on our improving knowledge about plant-microorganism interactions and the availability of genetic engineering methods have yielded inoculants with potentially improved properties (9,58). Before commercialization of such genetically modified organisms (GMO), their environmental safety should be demonstrated.…”

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confidence: 99%

Effect of Field Inoculation with Sinorhizobium meliloti L33 on the Composition of Bacterial Communities in Rhizospheres of a Target Plant ( Medicago sativa ) and a Non-Target Plant ( Chenopodium album )—Linking of 16S rRNA Gene-Based Single-Strand Conformation Polymorphism Community Profiles to the Diversity of Cultivated Bacteria

Schwieger

Tebbe

2000

Appl Environ Microbiol

129

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Fourteen weeks after field release of luciferase gene-tagged Sinorhizobium meliloti L33 in field plots seeded with Medicago sativa, we found that the inoculant also occurred in bulk soil from noninoculated control plots. In rhizospheres of M. sativa plants, S. meliloti L33 could be detected in noninoculated plots 12 weeks after inoculation, indicating that growth in the rhizosphere preceded spread into bulk soil. To determine whether inoculation affected bacterial diversity, 1,119 bacteria were isolated from the rhizospheres of M. sativa and Chenopodium album, which was the dominant weed in the field plots. Amplified ribosomal DNA restriction analysis (ARDRA) revealed plant-specific fragment size frequencies. Dominant ARDRA groups were identified by 16S rRNA gene nucleotide sequencing. Database comparisons indicated that the rhizospheres contained members of the Proteobacteria (␣, ␤, and ␥ subgroups), members of the Cytophaga-Flavobacterium group, and gram-positive bacteria with high G؉C DNA contents. The levels of many groups were affected by the plant species and, in the case of M. sativa, by inoculation. The most abundant isolates were related to Variovorax sp., Arthrobacter ramosus, and Acinetobacter calcoaceticus. In the rhizosphere of M. sativa, inoculation reduced the numbers of cells of A. calcoaceticus and members of the genus Pseudomonas and increased the number of rhizobia. Cultivation-independent PCR-single-strand conformation polymorphism (SSCP) profiles of a 16S rRNA gene region confirmed the existence of plant-specific rhizosphere communities and the effect of the inoculant. All dominant ARDRA groups except Variovorax species could be detected. On the other hand, the SSCP profiles revealed products which could not be assigned to the dominant cultured isolates, indicating that the bacterial diversity was greater than the diversity suggested by cultivation.

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Expression ofSerratia marcescensChitinase Gene inRhizobium melilotiDuring Symbiosis on Alfalfa Roots

Cited by 30 publications

References 0 publications

A Review of the Applications of Chitin and Its Derivatives in Agriculture to Modify Plant-Microbial Interactions and Improve Crop Yields

A Review of the Applications of Chitin and Its Derivatives in Agriculture to Modify Plant-Microbial Interactions and Improve Crop Yields

Recombinants Rhizobacteria Enhanced Biological Control of Rhizoctonia solani infected Faba Bean

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