Expression of Class II Chitinase Gene in Chilli (Capsicum annuum L.) as Response to Fusarium oxysporum Pathogen Attack

Abstract

Chilli plants have many mechanism defenses to pathogen attack. The biotic stress can induce genes encoding Pathogenesis Related (PR) proteins to increase expression. The aim of this research is to analyze a molecular responds of CaChi2 gene as PR protein in chilli, which is infected by fungal pathogenic Fusarium oxysporum. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to measure the fluorescence and the ddCt method was used to analyze the expression level of CaChi2 in chilli. The result showed, up-regulated expression of resistant plants but no change in expression of susceptible plants after many range of time. It is concluded that the CaChi2 gene can be a molecular marker of resistance to Fusarium oxysporum in chilli.

Suppression of Pepper Root Rot and Wilt Diseases Caused by Rhizoctonia solani and Fusarium oxysporum

“…Additionally, exalted expressions of defense-related genes, PR1 and PR2, have been described in plants treated with PGPF [9]. Various defense mechanisms against pathogen infection were explored in chili plants [10]. The CaChi2 gene was involved in resistance to F. oxysporum f.sp.…”

An Optimised TRIzol-based Protocol for the Improvement of RNA Extraction Yield of Tomato Stem

“…Various molecular investigations such as quantitative trait mapping, mutant screening, and gene expression analysis in tomatoes began to be carried out in the early 20th century (Giovannoni, 2004). One of the essential investigations is the molecular analysis of plant response to the environment, such as drought stress (Zhou et al, 2019), pathogen attack, bacterial wilt (Hyakumachi et al, 2012), and Fusarium wilt (Ferniah et al, 2015;Jaiswal et al, 2020). Analysis of plant responses in various stress conditions is studied through gene expression in plant organs, including stem organs.…”