Expression of adiponectin and its receptors (AdipoR1 and AdipoR2) in goat ovary and its effect on oocyte nuclear maturation in vitro

Oliveira, Bruna Sabino Pinho de; Costa, Joana A.S.; Gomes, Elizabete Teixeira; Silva, Diogo M.F.; Torres, Sandra Maria de; Monteiro, P. L. J.; Filho, A. S. Santos; Guerra, Maria Madalena Pessoa; Carneiro, Gustavo Ferrer; Wischral, Áurea; Batista, André Mariano

doi:10.1016/j.theriogenology.2017.08.013

Cited by 18 publications

(13 citation statements)

References 28 publications

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“…The results of the present study demonstrate that the addition of recombinant adiponectin (5 μg/ml) in the IVM medium increased the meiotic maturation rate of goat oocytes. These results are consistent with initial studies that demonstrated an increase in the proportion of goat oocytes reaching the stage of MII by treatment with adiponectin (5 and 10 μg/ml) in serum‐free maturation medium (Oliveira et al, ). However, controversial results have been reported in the literature.…”

Section: Discussionsupporting

confidence: 92%

“…This experiment was designed to confirm and extend the results of previous study(Oliveira et al, 2017). The COCs (n = 390 oocytes) were randomly distributed in groups of 20-25 in 90-μl drops of serum-free maturation medium, with or without adiponectin (0 or5 μg/ml), or in 10% (v/v) foetal bovine serum (FBS) as positive control.…”

mentioning

confidence: 86%

“…Ovarian expression of both receptors has been demonstrated in several species (humans: Chabrolle et al, ; rodents: Chabrolle, Tosca, & Dupont, ; porcine: Lord, Ledoux, Murphy, Beaudry, & Palin, ; chickens: Chabrolle, Tosca, Crochet, Tesseraud, & Dupont, ; bovine: Tabandeh, Hosseini, Saeb, Kafi, & Saeb, ). In addition, our team has found that both receptors are also present in goat ovaries and oocytes (Oliveira et al, ). All these data suggest that adiponectin could play an important role in goat oocyte maturation.…”

Section: Introductionmentioning

confidence: 94%

“…Previous studies have shown that adiponectin supplementation during in vitro maturation (IVM) of human, mouse and swine oocytes exerts positive effects on meiotic progression and initial embryonic development (Chappaz et al, ; Richards et al, ). In addition, our previous experiments demonstrated that the addition of physiological concentrations of human recombinant adiponectin (5 or 10 μg/ml) to a serum‐free medium for goat IVM improved rates of nuclear maturation of oocytes (Oliveira et al, ). In contrast, no significant effects of adiponectin were observed on bovine in vitro oocyte maturation, cleavage and blastocyst formation rates (Divar, Kafi, Mohammadi, & Azari, ; Maillard et al, ).…”

Section: Introductionmentioning

confidence: 98%

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Effects of adiponectin during in vitro maturation of goat oocytes: MEK 1/2 pathway and gene expression pattern

Gomes

Costa

Silva

et al. 2018

Reprod Domestic Animals

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Recent studies have shown that adiponectin, an adipokine predominantly produced by adipose tissue, regulates several reproductive processes. However, the mechanisms of action of adiponectin on the maturation of goat oocytes remain to be determined. The aim of this study was to investigate whether (a) adiponectin influences the meiotic maturation of goat oocytes; (b) MAPK MEK 1/2 mediates the effects of adiponectin; and 3) adiponectin differentially affects mRNA relative abundance of genes relevant for adiponectin signal transduction in goat oocytes. The addition of adiponectin (5 μg/ml) during the maturation of goat oocytes resulted in a higher percentage of successful nuclear maturation compared to those of the group without adiponectin (p < 0.05). Adiponectin-stimulated nuclear oocyte maturation was significantly impaired by a mitogen-activated protein kinase MEK 1/2 inhibitor, U0126 (p < 0.05). There was no evidence of any adiponectin-induced difference in the relative transcript abundances of AdipoR1, AdipoR2, AMPKα1, AMPKα2, PPARα and PPARγ genes. In conclusion, these results indicate that adiponectin has a positive effect on the meiotic maturation of goat oocytes through the MAPK MEK 1/2 pathway. Furthermore, the adiponectin does not affect the relative abundance of genes relevant for adiponectin signal transduction in goat oocytes.

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Section: Discussionsupporting

confidence: 92%

mentioning

confidence: 86%

Section: Introductionmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 98%

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Effects of adiponectin during in vitro maturation of goat oocytes: MEK 1/2 pathway and gene expression pattern

Gomes

Costa

Silva

et al. 2018

Reprod Domestic Animals

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“…Evidence has also shown the involvement of adiponectin directly at the peripheral level on the ovary, uterus and placenta (Caminos et al, ; Dobrzyn et al, ; Reverchon et al, ). Expression of adiponectin and its receptors (AdipoR1 and AdipoR2) have been demonstrated in the ovary of rat (Chabrolle, Tosca, & Dupont, ), chicken (Chabrolle, Tosca, Crochet, Tesseraud, & Dupont, ), human (Chabrolle et al, ), bovine (Tabandeh, Hosseini, Saeb, Kafi, & Saeb, ), porcine (Maleszka, Smolinska, Nitkiewicz, Kiezun, Chojnowska, et al, ; Maleszka, Smolinska, Nitkiewicz, Kiezun, Dobrzyn, et al, ) and caprine (Oliveira et al, ). Recent research findings have shown that adiponectin affects the steroidogenesis and proliferation of ovarian cells.…”

Section: Introductionmentioning

confidence: 99%

Expression and localization of adiponectin and its receptors in ovarian follicles during different stages of development and the modulatory effect of adiponectin on steroid production in water buffalo

Gupta¹,

Bahiram²,

Sardar³

et al. 2019

Reprod Domestic Animals

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Adiponectin is an adipocyte-derived hormone regulating energy metabolism, insulin sensitivity and recently found to regulate reproduction. The current study was carried out to investigate gene and protein expression, immunolocalization of adiponectin and its receptors AdipoR1 and AdipoR2 in ovarian follicles of different developmental stages in water buffalo (Bubalus bubalis) and to investigate the effect of adiponectin on steroid production in cultured bubaline granulosa cells. qPCR, western blotting and immunohistochemistry were applied to demonstrate mRNA expression, protein expression and immunolocalization, respectively. The results indicate that adiponectin, AdipoR1 and AdipoR2 were present in granulosa cells (GC) and theca interna (TI) of ovarian follicles and the expression of adiponectin, AdipoR1, AdipoR2 in GC and AdipoR1 and AdipoR2 in TI increased with increase in follicle size (p < .05). Expression of adiponectin was high in small and medium size follicles in TI. The adiponectin and its receptors were immunolocalized in the cytoplasm of GC and TI cells. Further, in the in-vitro study, GCs were cultured and treated with recombinant adiponectin each at 0, 1 and 10 µg/ml alone or with follicle stimulating hormone (FSH) at 30 ng/ml) or Insulin-like growth factor I (IGF-I) at 10 ng/ml for 48 hr after obtaining 75%-80%s confluency. Adiponectin at 10 µg/ml increased IGF-I-induced estradiol (E 2 ) and progesterone (P 4 ) secretion and FSH-induced E 2 secretion from GC and also increased the abundance of factors involved in E 2 and P 4 production (cytochrome P45019A1 [CYP19A1] and 3-beta-hydroxysteroid dehydrogenase [3β-HSD]). In conclusion, this study provides novel evidence for the presence of adiponectin and its receptors in ovarian follicles and modulatory role of adiponectin on steroid production in buffalo.

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Interplay of adipocytokines with polycystic ovary syndrome

Baig¹,

Ahmad²,

Tariq³

2024

Polycystic Ovary Syndrome

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Expression of adiponectin and its receptors (AdipoR1 and AdipoR2) in goat ovary and its effect on oocyte nuclear maturation in vitro

Cited by 18 publications

References 28 publications

Effects of adiponectin during in vitro maturation of goat oocytes: MEK 1/2 pathway and gene expression pattern

Effects of adiponectin during in vitro maturation of goat oocytes: MEK 1/2 pathway and gene expression pattern

Expression and localization of adiponectin and its receptors in ovarian follicles during different stages of development and the modulatory effect of adiponectin on steroid production in water buffalo

Interplay of adipocytokines with polycystic ovary syndrome

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