Effects of adiponectin during in vitro maturation of goat oocytes: MEK 1/2 pathway and gene expression pattern

Abstract

Recent studies have shown that adiponectin, an adipokine predominantly produced by adipose tissue, regulates several reproductive processes. However, the mechanisms of action of adiponectin on the maturation of goat oocytes remain to be determined. The aim of this study was to investigate whether (a) adiponectin influences the meiotic maturation of goat oocytes; (b) MAPK MEK 1/2 mediates the effects of adiponectin; and 3) adiponectin differentially affects mRNA relative abundance of genes relevant for adiponectin signal transduction in goat oocytes. The addition of adiponectin (5 μg/ml) during the maturation of goat oocytes resulted in a higher percentage of successful nuclear maturation compared to those of the group without adiponectin (p < 0.05). Adiponectin-stimulated nuclear oocyte maturation was significantly impaired by a mitogen-activated protein kinase MEK 1/2 inhibitor, U0126 (p < 0.05). There was no evidence of any adiponectin-induced difference in the relative transcript abundances of AdipoR1, AdipoR2, AMPKα1, AMPKα2, PPARα and PPARγ genes. In conclusion, these results indicate that adiponectin has a positive effect on the meiotic maturation of goat oocytes through the MAPK MEK 1/2 pathway. Furthermore, the adiponectin does not affect the relative abundance of genes relevant for adiponectin signal transduction in goat oocytes.

Interstitial Leydig Cell Tumorigenesis—Leptin and Adiponectin Signaling in Relation to Aromatase Expression in the Human Testis

“…It was demonstrated that cancers acquire genetic mutations and epigenetic modifications that can result in the activation of oncogenes e.g., Raf and ERK, or can lead to inactivation of tumor suppressors [67]. Of note, through ERK pathway leptin influenced cumulus maturation and steroidogenic secretion in rabbit [68] and adiponectin in goat [69]. As mentioned above, incomplete data reflects distinct adipokine signaling pathways and/or single molecules of adipokine signaling can take part in Leydig cell regulation under central hypothalamus-pituitary-gonadal axis and via local lipid-and estrogen-created microenvironment [70,71].…”

Mechanisms of Adiponectin Action in Fertility: An Overview from Gametogenesis to Gestation in Humans and Animal Models in Normal and Pathological Conditions

“…The expression of adiponectin (gene and protein) was found in the oocytes of rats [17] and cows [77,84], whereas that of AdipoR1 and AdipoR2 has been shown in oocytes of cows [84], pigs [104], goats [105] and rats [17]. Several studies have shown that adiponectin supplementation during in vitro maturation (IVM) of human, mouse, goat and swine oocytes exerts positive effects on meiotic progression and initial embryonic development [104,106,107] (Figure 4A).…”

“…Several studies have shown that adiponectin supplementation during in vitro maturation (IVM) of human, mouse, goat and swine oocytes exerts positive effects on meiotic progression and initial embryonic development [104,106,107] (Figure 4A). In goat oocytes, adiponectin has a positive effect on the meiotic maturation through the classical MAPK pathway [105]. In contrast, no significant effects of adiponectin were observed on bovine IVM, cleavage and blastocyst formation rates [84] (Figure 4A).…”

Expression and localization of adiponectin and its receptors in ovarian follicles during different stages of development and the modulatory effect of adiponectin on steroid production in water buffalo

“…It is also demonstrated that recombinant adiponectin increases in‐vitro nuclear maturation of oocytes in porcine and caprine species (Chappaz et al, ; Gomes et al, ; Oliveira et al, ) . In our recent study, we observed that adiponectin increases cellular proliferation of GCs in presence of FSH and IGF‐I and in luteal cells it has no effect on cellular proliferation or apoptosis (Gupta, Thakre, Korde, et al, ).…”