Exploring Three PIPs and Three TIPs of Grapevine for Transport of Water and Atypical Substrates through Heterologous Expression in aqy-null Yeast

Sabir, Farzana; Leandro, Maria; Martins, Ana Paula; Loureiro-Dias, Maria C.; Moura, Teresa; Soveral, Graça; Prista, Catarina

doi:10.1371/journal.pone.0102087

Cited by 31 publications

(34 citation statements)

References 66 publications

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“…Yeast cells expressing Vitis aquaporins were osmosensitive (Sabir et al 2014). In this study, 16 PIP genes were down-regulated by ethylene treatment in AZ-C (Fig.…”

Section: Transportation Of Materials Is Differentially Regulated By Ementioning

confidence: 50%

Profiling gene expression in citrus fruit calyx abscission zone (AZ-C) treated with ethylene

et al. 2015

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On-tree storage and harvesting of mature fruit account for a large proportion of cost in the production of citrus, and a reduction of the cost would not be achieved without a thorough understanding of the mechani sm of the mature fruit abscission. Genome-wide gene expression changes in ethylene-treated fruit calyx abscission zone (AZ-C) of Citrus sinensis cv. Olinda were therefore investigated using a citrus genome array representing up to 33,879 citrus transcripts. In total, 1313 and 1044 differentially regulated genes were identified in AZ-C treated with ethylene for 4 and 24 h, respectively. The results showed that mature citrus fruit abscission commenced with the activation of ethylene signal transduction pathway that led to the activation of ethylene responsive transcription factors and the subsequent transcriptional regulation of a large set of ethylene responsive genes. Significantly down-regulated genes included those of starch/sugar biosynthesis, transportation of water and growth promoting hormone synthesis and signaling, whereas significantly up-regulated genes were those involved in defense, cell wall degradation, and secondary metabolism. Our data unraveled the underlying mechanisms of some known important biochemical events occurring at AZ-C and should provide informative suggestions for future manipulation of the events to achieve a controllable abscission for mature citrus fruit.

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“…Yeast cells expressing Vitis aquaporins were osmosensitive (Sabir et al 2014). In this study, 16 PIP genes were down-regulated by ethylene treatment in AZ-C (Fig.…”

Section: Transportation Of Materials Is Differentially Regulated By Ementioning

confidence: 50%

Profiling gene expression in citrus fruit calyx abscission zone (AZ-C) treated with ethylene

et al. 2015

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“…Transcript analysis showed that all of these genes are involved in the response to drought. Water channel activity is typically observed in plasma membrane intrinsic proteins (PIPs) and tonoplast intrinsic proteins (TIPs) (Thorsten et al 2011).In grapevine, TIPs exhibit higher water conductivity than PIPs (Sabir et al 2014). In the current study, the expression of aquaporin genes in leaves in both types of apple increased after drought treatment, implying that these genes may play a positive role in mediating responses to drought stress.…”

Section: Discussionmentioning

confidence: 99%

Autotetraploidization enhances drought stress tolerance in two apple cultivars

Feng

Hou

et al. 2015

Trees

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Key message It reports relevant traits of diploid and autotetraploid apple plants ('Hanfu' and 'Gala') in response to drought. The differences indicate that apple plants ('Hanfu' and 'Gala') may differ in their response to drought stress depending on ploidy level. Abstract The induction of polyploidy ameliorates the adverse effects of drought in many plants. In this study, the tolerance responses of diploid and autotetraploid apple with two cultivars were compared under drought stress treatment induced by polyethylene glycol (PEG)-6000 in the laboratory. Autotetraploid apple plants were previously induced in vitro from the diploid apple (Malus 9 domestica, 2n = 2x = 34) cultivar 'Hanfu' and 'Gala' by colchicine treatment. In this study, we elucidated the effects of drought stress on apple by investigating the following parameters in leaves: relative water content (RWC), proline content, malondialdehyde (MDA) content and expression of key aquaporin genes. Under drought stress, autotetraploid apple had higher RWC and chlorophyll fluorescence parameters and lower levels of MDA and proline compared with diploid apple. Key aquaporins genes were induced in leaves in response to PEG6000 treatment, including MdPIP1;1 and MdTIP1;1. The expression of these genes induced under drought stress treatment, and the genes were expressed at lower levels in the autotetraploid than in the diploid. Our combined physiological and molecular data reveal that polyploidization can enhance drought tolerance in 'Hanfu' and 'Gala' apple.

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“…Use of florescent dye in stopped-flow spectrometry increases precision of measurements. Recently, Sabir et al (2014) evaluated grape AQPs for water conductivity using a stopped-flow fluorescence spectroscopy assay. They pre-loaded yeast cells with the non-fluorescent precursor 5-(and-6)-carboxyfluorescein diacetate (CFDA) that is permeable to membranes, and then intracellularly hydrolyzed CFDA to release the membrane impermeable fluorescent compound.…”

Section: Analytical and Functional Approachesmentioning

confidence: 99%

Plant Aquaporins: Genome-Wide Identification, Transcriptomics, Proteomics, and Advanced Analytical Tools

2016

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Aquaporins (AQPs) are channel-forming integral membrane proteins that facilitate the movement of water and many other small molecules. Compared to animals, plants contain a much higher number of AQPs in their genome. Homology-based identification of AQPs in sequenced species is feasible because of the high level of conservation of protein sequences across plant species. Genome-wide characterization of AQPs has highlighted several important aspects such as distribution, genetic organization, evolution and conserved features governing solute specificity. From a functional point of view, the understanding of AQP transport system has expanded rapidly with the help of transcriptomics and proteomics data. The efficient analysis of enormous amounts of data generated through omic scale studies has been facilitated through computational advancements. Prediction of protein tertiary structures, pore architecture, cavities, phosphorylation sites, heterodimerization, and co-expression networks has become more sophisticated and accurate with increasing computational tools and pipelines. However, the effectiveness of computational approaches is based on the understanding of physiological and biochemical properties, transport kinetics, solute specificity, molecular interactions, sequence variations, phylogeny and evolution of aquaporins. For this purpose, tools like Xenopus oocyte assays, yeast expression systems, artificial proteoliposomes, and lipid membranes have been efficiently exploited to study the many facets that influence solute transport by AQPs. In the present review, we discuss genome-wide identification of AQPs in plants in relation with recent advancements in analytical tools, and their availability and technological challenges as they apply to AQPs. An exhaustive review of omics resources available for AQP research is also provided in order to optimize their efficient utilization. Finally, a detailed catalog of computational tools and analytical pipelines is offered as a resource for AQP research.

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Exploring Three PIPs and Three TIPs of Grapevine for Transport of Water and Atypical Substrates through Heterologous Expression in aqy-null Yeast

Cited by 31 publications

References 66 publications

Profiling gene expression in citrus fruit calyx abscission zone (AZ-C) treated with ethylene

Profiling gene expression in citrus fruit calyx abscission zone (AZ-C) treated with ethylene

Autotetraploidization enhances drought stress tolerance in two apple cultivars

Plant Aquaporins: Genome-Wide Identification, Transcriptomics, Proteomics, and Advanced Analytical Tools

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