Exploring the Genetic Diversity and Population Structure of Turkish Laurel Germplasm by the iPBS-Retrotransposon Marker System

Abstract

Laurel is a medicinally important plant and is known to the world for its essential oil. Turkey is the main market in the laurel leaf trade by sharing about 90% of the world trade. Here we made an effort to elucidate genetic diversity and population structure of 94 Turkish laurel genotypes collected from 26 provinces and four geographical regions using inter-primer binding site (iPBS) retrotransposon markers. A total of 13 most polymorphic primers were selected which yielded 195 total bands, of which 84.10% were found polymorphic. Mean polymorphism information content (PIC) was (0.361) and diversity indices including mean effective number of alleles (1.36), mean Shannon's information index (0.35) and overall gene diversity (0.22) revealed the existence of sufficient amount of genetic diversity in the studied plant material. Most diversity was found in genotypes collected from the Mediterranean region. Analysis of molecular variance (AMOVA) revealed that most of the variation (85%) in Turkish laurel germplasm is due to differences within populations. Model-based structure, principal coordinate analysis (PCoA) and neighbor-joining algorithms were found in agreement and clustered the studied germplasm according to their collection provinces and regions. This is a very first study exploring the genetic diversity and population structure of laurel germplasm using iPBS-retrotransposon marker system. We believe that information provided in this work will be helpful for the scientific community to take more interest in this forgotten but the medicinally important plant.

DNA fingerprinting and genetic diversity analysis of world quinoa germplasm using iPBS-retrotransposon marker system

“…iPBS-retrotransposon is a PCR-based marker system depending on the presence of tRNA as a reverse transcriptase primer binding site (Kalendar et al, 2010). This marker system has been successfully utilized for the investigation of genetic diversity and population structure of various crops like pea (Baloch et al, 2015), common bean (Aydin and Baloch, 2019;Nemli et al, 2015), ciccer (Andeden et al, 2013), laurel (Karık et al, 2019), and pepper (Yildiz et al, 2019). This universal marker system has also been utilized for the genetic characterization of quinoa germplasm using 17 accessions cultivated in Turkey (Hossein-Pour et al, 2019).…”

Genetic Relationships and Diversity Analysis in Turkish Laurel (Laurus Nobilis L.) Germplasm Using ISSR and SCoT Markers

“…In brief, limited number of molecular characterization studies have been performed related to this subject on local laurel genotypes. However, the germplasm size or/and the number of molecular markers used previously are relatively small except for the work of Karik et al [14]. Therefore, more extensive regional studies were requiredThere are only two studies that have been conducted with ISSR markers so far [15,16], but the research area sizes were very small compared to this study.…”

Inter-primer binding site (iPBS) markers reveal the population genetic diversity and structure of tropical climbing Cissampelopsis (Asteraceae) in Thailand

“…Since the development of applicable iPBS procedures, this marker has been widely used for population genetic diversity and structures analysis (Baloch et al 2015;Nemli et al 2015;Hossein-Pour et al 2019;Karık et al 2019;Karagoz et al 2020). This is of advantage because polymorphisms detected by iPBS are expected to be less biased estimators of genetic difference than variation at the level of gene products (Kalendar et al 2019).…”

“…Due to this, the iPBS technique is an easy-to-use technique that requires no sequence data, cost-effective, not age or tissue-specific, highly informative, and no affected by environmental influences (Nemli et al 2015;Amom et al 2020). Thus, the iPBS retrotransposon technique has been selected as a marker to examine population genetic diversity and structure in many plant genera such as Cicer (Andeden et al 2013), Vitis (Guo et al 2014), Pisum (Baloch et al 2015), Phaseolus (Nemli et al 2015), Psidium (Mehmood et al 2017), Castanea, Fagus and Quercus (Coutinho et al 2018), Chenopodium (Hossein-Pour et al 2019), Laurus (Karık et al 2019), Hordeum (Bonchev et al 2019), Origanum (Karagoz et al 2020). Moreover, in Asteraceae iPBS method has been proved to be a reliable marker for the evaluation of genetic diversity at infraspecific level, as well as the intergeneric hybrid in several genera (Gailite and Rungis 2012;Ali et al 2019;Bonchev and Vassilevska-Ivanova 2020).…”