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A revision of the Asian genus Cissampelopsis (DC.) Miq. is presented. Two sections with ten species and two varieties are recognised. These are sect.

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A revision of the paleotropical genus Gynura Cass. is presented. Forty-four species are recognized, of which three are described as new.

Abstract. Vanijajiva O. 2020. Start codon targeted (SCoT) polymorphism reveals genetic diversity of Manilkara in Thailand. Biodiversitas 21: 666-673. Manilkara consists of four species, namely, Manilkara hexandra, M. kauki, M. littoralis, and M. zapota in Thailand. Particularly, M. zapota (sapodilla), commonly known as Lamut in Thailand, is one of commercially notable edible fruits continues to be a highly valued tree and the fruits are a source of food and income. The objective of this research was to determine the diversity and relationship of Manilkara accessions derived from districts in Thailand based on DNA profiles. Genetic diversity and relationship of all accessions were investigated using Start Codon Targeted (SCoT) marker analysis. Of 30 SCoT primers screened, 27 SCoT primers were identified to be polymorphism. A total of 176 DNA bands with size varied from 120 bp to 1200 bp were amplified, and 101 (57.38%) of them were polymorphic. Based on similarity coefficients, total of 36 accessions were clustered into two groups. The cluster of polymorphism generated by SCoT was associated with their locality and morphological characters. Therefore, the present data provide high-valued information for the management of germplasm, genetic improvement, and conservation of the genetic resources of Manilkara accessions.

Bouea macrophylla Griff. is well-known as one of native typical fruits in Southeast Asia which needs to be preserved and continuously cultivated because of economical and ecological significances. More recently, sequence-related amplified polymorphism (SRAP) markers have been developed, which are used to amplify coding regions of DNA with primers targeting open reading frames. This technique has proven to be robust and highly variable and is attained through a significantly less technically demanding process. In this research, SRAP method was preliminary applied to assess genetic characterization of B. macrophylla. Genomic DNA was extracted from fresh leaf samples. The result clearly showed that at 100 ng template DNA and MgCl 2 5 mM concentration are suitable for further PCR analysis. Thirty SRAP primer combinations were initially screened for analysis and 26 primer combinations were chosen for further analysis. A total of 222 DNA fragments, varying from 90-2500 bp, were amplified. The produced band number for each optimal primer set ranged from 3 to 12 with a percentage of polymorphic bands spanning from 33.33 to 80.00%. Therefore, SRAP analysis is suitable for further analysis method on genetic study of Bouea species and related genera.

The genus Crassocephalum in Asia, introduced there from Africa, was examined by extensive field work, herbarium studies, analyses of pollen and seed fertility, chromosome counts and ITS and trnL-F sequencing. We found that Crassocephalum in Asia comprises two species and their interspecific hybrid. The two species are C. crepidioides (Benth.) S. Moore and C. rubens (Juss. exJacq.) S. Moore, of which the latter is a new record for Asia (north Thailand). The hybrid between these two species in north Thailand originated from a cross between C. crepidioides (2n = 40) as female and C. rubens (2n = 40) as male parent.