Morphological and molecular evidence for interspecific hybridisation in the introduced African genus<i>Crassocephalum</i>(Asteraceae: Senecioneae) in Asia

Vanijajiva, Ongkarn; Kadereit, Joachim W.

doi:10.1017/s147720000900303x

Cited by 11 publications

(4 citation statements)

References 17 publications

(20 reference statements)

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“…C.c. Ile-Ife contained 40 chromosomes ( Rozhon et al, 2018 ), which indicated tetraploidy, and was in line with previous reports ( Vanijajiva and Kadereit, 2009 ; Oyelakin and Ayodele, 2013 ). The ploidy level of C. rubens was described as diploid ( Oyelakin and Ayodele, 2013 ).…”

Section: Resultssupporting

confidence: 92%

The Orphan Crop Crassocephalum crepidioides Accumulates the Pyrrolizidine Alkaloid Jacobine in Response to Nitrogen Starvation

et al. 2021

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Crassocephalum crepidioides is an African orphan crop that is used as a leafy vegetable and medicinal plant. Although it is of high regional importance in Sub-Saharan Africa, the plant is still mainly collected from the wild and therefore efforts are made to promote its domestication. However, in addition to beneficial properties, there was first evidence that C. crepidioides can accumulate the highly toxic pyrrolizidine alkaloid (PA) jacobine and here it was investigated, how jacobine production is controlled. Using ecotypes from Africa and Asia that were characterized in terms of their PA profiles, it is shown that the tetraploid C. crepidioides forms jacobine, an ability that its diploid close relative Crassocephalum rubens appears to lack. Evidence is provided that nitrogen (N) deficiency strongly increases jacobine in the leaves of C. crepidioides, that this capacity depends more strongly on the shoot than the root system, and that homospermidine synthase (HSS) activity is not rate-limiting for this reaction. A characterization of HSS gene representation and transcription showed that C. crepidioides and C. rubens possess two functional versions, one of which is conserved, that the HSS transcript is mainly present in roots and that its abundance is not controlled by N deficiency. In summary, this work improves our understanding of how environmental cues impact PA biosynthesis in plants and provides a basis for the development of PA-free C. crepidioides cultivars, which will aid its domestication and safe use.

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Section: Resultssupporting

confidence: 92%

The Orphan Crop Crassocephalum crepidioides Accumulates the Pyrrolizidine Alkaloid Jacobine in Response to Nitrogen Starvation

et al. 2021

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“…The identity of the collected material was confirmed by PCR and sequencing of the ITS of the nuclear rRNA gene (Blattner, ) and the chloroplast trnL ‐ trnF intergenic region (Taberlet et al ., ). According to the literature (Gadella, ; Vanijajiva et al ., ), ecotypes of C. crepidioides are usually tetraploid (4n) with 40 chromosomes. Staining of root tips with orcein and subsequent light microscopy (see Supporting Information Fig.…”

Section: Resultsmentioning

confidence: 99%

Quantification of the Pyrrolizidine Alkaloid Jacobine in Crassocephalum crepidioides by Cation Exchange High‐Performance Liquid Chromatography

Rozhon

Kammermeier

Schramm

et al. 2017

Phytochemical Analysis

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ABSTRACT:Introduction -Pyrrolizidine alkaloids (PAs) are secondary plant metabolites with considerable hepatoxic, tumorigenic and genotoxic potential. For separation, reversed phase chromatography is commonly used because of its excellent compatibility with detection by mass spectrometry. However, reversed phase chromatography has a low selectivity for PAs. Objective -The objective of this work was to investigate the suitability of cation exchange chromatography for separation of PAs and to develop a rapid method for quantification of jacobine in Crassocephalum crepidioides that is suitable for analysis of huge sample numbers as required for mutant screening procedures. Results -We demonstrate that cation exchange chromatography offers excellent selectivity for PAs allowing their separation from most other plant metabolites. Due to the high selectivity, plant extracts can be directly analysed after simple sample preparation. Detection with UV at 200 nm instead of mass spectrometry can be applied, which makes the method very simple and cost-effective. The recovery rate of the method exceeded 95%, the intra-day and inter-day standard deviations were below 7% and the limit of detection and quantification were 1 mg/kg and 3 mg/kg, respectively. Conclusion -The developed method is sufficiently sensitive for reproducible detection of jacobine in C. crepidioides. Simple sample preparation and rapid separation allows for quantification of jacobine in plant material in a high-throughput manner. Thus, the method is suitable for genetic screenings and may be applicable for other plant species, for instance Jacobaea maritima. In addition, our results show that C. crepidioides cannot be considered safe for human consumption.

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“…Hybridization events may also be difficult to recognize when they result in homoploid hybrids, are ancient, or were followed by speciation or dispersal in combination with extinction in the parental distribution area. Hybridization has been demonstrated within many Senecioneae genera (e.g., Bedfordia, Blennosperma, Brachyglottis, Cineraria, Crassocephalum, Dendrosenecio, Dolichoglottis, Emilia, Euryops, Farfugium, Hubertia, Jacobaea, Jessea, Ligularia, Packera, Petasites, Senecio, Traversia ; Nordenstam, 1963, 1968, 1978, 1996; Ornduff, 1964; Chapman and Jones, 1971; Drury, 1973; Olorode and Olorunfemi, 1973; Jeffrey, 1986; Yamaguchi and Yahara, 1989; Beck et al, 1992; Lowe and Abbott, 2000; Kirk et al, 2004; Kadereit et al, 2006; Cron et al, 2008; Pan et al, 2008; Vanijajiva and Kadereit, 2009) and is therefore a likely hypothesis for explaining incongruence between plastid and ITS/ETS phylogenies.…”

Section: Discussionmentioning

confidence: 99%

Patterns and causes of incongruence between plastid and nuclear Senecioneae (Asteraceae) phylogenies

Pelser

Kennedy

Tepe

et al. 2010

American J of Botany

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One of the longstanding questions in phylogenetic systematics is how to address incongruence among phylogenies obtained from multiple markers and how to determine the causes. This study presents a detailed analysis of incongruent patterns between plastid and ITS/ETS phylogenies of Tribe Senecioneae (Asteraceae). This approach revealed widespread and strongly supported incongruence, which complicates conclusions about evolutionary relationships at all taxonomic levels. The patterns of incongruence that were resolved suggest that incomplete lineage sorting (ILS) and/or ancient hybridization are the most likely explanations. These phenomena are, however, extremely difficult to distinguish because they may result in similar phylogenetic patterns. We present a novel approach to evaluate whether ILS can be excluded as an explanation for incongruent patterns. This coalescence-based method uses molecular dating estimates of the duration of the putative ILS events to determine if invoking ILS as an explanation for incongruence would require unrealistically high effective population sizes. For four of the incongruent patterns identified within the Senecioneae, this approach indicates that ILS cannot be invoked to explain the observed incongruence. Alternatively, these patterns are more realistically explained by ancient hybridization events.

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Morphological and molecular evidence for interspecific hybridisation in the introduced African genusCrassocephalum(Asteraceae: Senecioneae) in Asia

Cited by 11 publications

References 17 publications

The Orphan Crop Crassocephalum crepidioides Accumulates the Pyrrolizidine Alkaloid Jacobine in Response to Nitrogen Starvation

The Orphan Crop Crassocephalum crepidioides Accumulates the Pyrrolizidine Alkaloid Jacobine in Response to Nitrogen Starvation

Quantification of the Pyrrolizidine Alkaloid Jacobine in Crassocephalum crepidioides by Cation Exchange High‐Performance Liquid Chromatography

Patterns and causes of incongruence between plastid and nuclear Senecioneae (Asteraceae) phylogenies

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