Exploring the genetic diversity and population structure of Turkish common bean germplasm by the iPBS-retrotransposons markers

Abstract

Present study was aimed to investigate the genetic diversity and population structure of Turkish common bean germplasm. A total of 96 bean genotypes were characterized with iPBS-retrotansposons that yielded a mean polymorphism information contents of 0.8. Mean gene diversity and Shannon information index were 0.14 and 0.25, respectively. Neighbor joining analysis divided the bean genotypes into two main group (A and B) according to their geographical regions, growth habits and seed size. Bingol-l7, Sivas-14 and Hakkari-11 genotypes were found very distinct and can be used as candidate parents for the bean breeding. The higher efficiency and reproducibility of iPBS-retrotransposons was witnessed in common bean as compared to earlier studies using the same marker system. Results of this study will boost up the investigators for genotyping the larger germplasm of common bean with minimum laboratory infrastructure in developing and least developed countries.

Exploring the Genetic Diversity and Population Structure of Turkish Laurel Germplasm by the iPBS-Retrotransposon Marker System

“…Mean polymorphism obtained in this study was 83.98%, which varied from 72.72% to 100%. Mean polymorphism obtained in this study was found greater than reported by Sevindik [47] for laurel, Yaldiz et al [25] for tobacco and Aydin and Baloch [48] using iPBS-retrotransposons and thus confirming greater variations in Turkish laurel germplasm. The PIC value is used to understand the efficiency of polymorphic loci for the identification of genetic diversity [43] and explore the discriminating power marker among genotypes [40].…”

“…Mean effective number of alleles (1.36) found in this study were higher than the reported by previous studies in various crops using iPBS-retrotransposons markers [25,50]. Mean gene diversity (0.22) resulted in this study was found higher than the reported by earlier studies [25,48,50] and thus explains the presence of higher diversity in studied germplasm. Shannon's information index is an important criterion to understand the variation as it distinguishes the genetic diversity in a population combining abundance and evenness [51].…”

“…Previous studies have proven iPBS-retrotransposon a highly reproducible, robust and trustable marker system [22,23,40,41]. Our earlier studies in wild emmer [8], pea [24], tobacco [25], common bean [48], pepper [50] and safflower [51] also found this marker system highly robust, and trustable, which can be used for the investigation of genetic diversity in any crop. Similarly, Mehmood et al [41] also found this marker system highly reproducible, robust and trustable.…”

Uncovering Phenotypic Diversity and DArTseq Marker Loci Associated with Antioxidant Activity in Common Bean

“…Earlier studies have confirmed the clustering of common bean germplasm based on plant height, 100-seed weight, and collection point. These previous reports strengthen the findings of our study [63].…”

Phenotypic Characterization of 183 Turkish Common Bean Accessions for Agronomic, Trading, and Consumer-Preferred Plant Characteristics for Breeding Purposes

“…Turkey is not a center of origin of common bean and it is supposed that it was introduced to Turkey from Europe in the 17th century by the Ottoman traders travelling between Europe and Asia [9][10][11]. Bean landraces present in small farmers' fields are playing a vital role in the country's economy [12,13]. Annual common bean production of Turkey was 235,000 tons versus nearly 26,833,394 tons of common bean produced worldwide [14].…”