Exploring the Characteristics of Intestinal Microbiota in Hematologic Malignancy Patients via 16s rDNA High-Throughput Sequencing

Zhong, Pei; Wang, Xiaowei; Li, Yajuan; Xu, Yuanhong

doi:10.7754/clin.lab.2020.200448

Cited by 5 publications

(6 citation statements)

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“…A further analysis of intestinal microorganisms in the ADC group mice revealed that the dominant differential species were Bacilli , Erysipelotrichales , Erysipelotrichaceae , and Allobaculum . Pei et al [ 67 ] showed that Erysipelotrichales and Erysipelotrichaceae species are increased in abundance in the intestine of patients with hematological malignancies and can be used as markers in these patients. A surge in the abundance of Erysipelotrichales is associated with intestinal inflammation [ 68 ] and blocking its growth in a model of parenteral nutrition-associated liver disease attenuates liver injury [ 69 ].…”

Section: Discussionmentioning

confidence: 99%

Treadmill Exercise Modulates Intestinal Microbes and Suppresses LPS Displacement to Alleviate Neuroinflammation in the Brains of APP/PS1 Mice

et al. 2022

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Neuroinflammation occurs throughout the pathogenesis of Alzheimer’s disease (AD). Here, we investigated the effects of treadmill exercise on neuroinflammation in APP/PS1 transgenic AD mice and the potential involvement of microbe–gut–brain axis (MGB) mechanisms based on growing evidence that AD’s pathogenesis is correlated with a deterioration in the function of gut microbiota. APP/PS1 transgenic AD mice were subjected to 12 weeks of treadmill exercise, followed by spatial memory tests. After the behavioral study, the amyloid (Aβ) pathology, gut microbes and metabolites, bacterial lipopolysaccharide (LPS) displacement, and degree of neuroinflammation were analyzed. We found that this strategy of exercise enriched gut microbial diversity and alleviated neuroinflammation in the brain. Notably, exercise led to reductions in pathogenic bacteria such as intestinal Allobaculum, increases in probiotic bacteria such as Akkermansia, increased levels of intestine–brain barrier proteins, and attenuated LPS displacement. These results suggest that prolonged exercise can effectively modulate gut microbes and the intestinal barrier and thereby reduce LPS displacement and ultimately alleviate AD-related neuroinflammation.

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Section: Discussionmentioning

confidence: 99%

Treadmill Exercise Modulates Intestinal Microbes and Suppresses LPS Displacement to Alleviate Neuroinflammation in the Brains of APP/PS1 Mice

et al. 2022

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“…These results revealed the significant different profiles of gut microbiota derived from AML patients and patients treated with chemotherapy or healthy subjects. Similarly, the genera Collinsella was found to be enriched in several malignant tumors, such as colorectal cancer, 36 gastric cancer, 37 esophageal cancer, 38 and hematological malignant 39 …”

Section: Discussionmentioning

confidence: 97%

“…Similarly, the genera Collinsella was found to be enriched in several malignant tumors, such as colorectal cancer, 36 gastric cancer, 37 esophageal cancer, 38 and hematological malignant. 39 Emerging evidences have demonstrated that metabolites generated by gut microbiota had the ability to modulate the physiological functions of the host. As reported by Zarour, gut microbiota-derived metabolites involve in immunoregulation in tumor.…”

Section: Collection Analysis Between Main Differential Metabolites An...mentioning

confidence: 99%

Alteration of gut microbiome and correlated amino acid metabolism are associated with acute myelocytic leukemia carcinogenesis

Kang

Zhong

et al. 2023

Cancer Medicine

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BackgroundThe aim of this study is to investigate the profiles of gut microbiota and metabolites in acute myelocytic leukemia (AML) patients treated with/without chemotherapy.MethodsHerein, high‐throughput 16S rRNA gene sequencing was performed to analysis gut microbiota profiles, and liquid chromatography and mass spectrometry were performed to analysis metabolites profiles. The correlation between gut microbiota biomarkers identified by LEfSe and differentially expressed metabolites were determined by spearman association analysis.ResultsThe results showed the distinguished gut microbiota and metabolites profiles between AML patients and control individuals or AML patients treated with chemotherapy. Compared to normal populations, the ratio of Firmicutes to Bacteroidetes was increased at the phylum level than that in AML patients, and LEfSe analysis identified Collinsella and Coriobacteriaceae as biomarkers of AML patients. Differential metabolite analysis indicated that, compared to AML patients, numerous differential amino acids and analogs could be observed in control individuals and AML patients treated with chemotherapy. Interestingly, spearman association analysis demonstrated that plenty of bacteria biomarkers shows statistical correlations with differentially expressed amino acid metabolites. In addition, we found that both Collinsella and Coriobacteriaceae demonstrate remarkable positive correlation with hydroxyprolyl‐hydroxyproline, prolyl‐tyrosine, and tyrosyl‐proline.ConclusionIn conclusion, our present study investigated the role of the gut‐microbiome–metabolome axis in AML and revealed the possibility of AML treatment by gut‐microbiome–metabolome axis in the further.

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“…In patients with liver cirrhosis, the abundance of Erysipelotrichaceae in the faecal microbiota signi cantly increases compared to that in nonliver cancer cirrhosis patients, and this change is associated with high expression of interleukin 6 (IL-6) and tumour necrosis factor alpha (TNF-α) [24]. Zhong Pei et al compared the faecal samples of patients with haematological malignancies to those of healthy individuals and reported a signi cant increase in Erysipelotrichaceae abundance, suggesting that this genus may be a biomarker for haematological malignancies [25]. In our study, Erysipelotrichaceae abundance was correlated strongly positively with the incidence of NK/T-cell lymphoma.…”

Section: Discussionmentioning

confidence: 99%

Causal Relationship between the Gut Microbiota and Haematological Malignancies: A Two-Sample Mendelian Randomization Analysis

Xiang,

Li,

Zhang

et al. 2024

Preprint

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Background The gut microbiota has been implicated in the occurrence and development of haematological malignancies. However, the causal relationship between specific gut microbiota and haematological malignancies remains unclear. Methods In this study, we employed the Mendelian randomization (MR) approach to investigate the causal relationship between the gut microbiota and haematological malignancies. We selected the human gut microbiota as the exposure variable from the human whole-genome association analysis (GWAS) dataset of International Alliance MiBioGen. GWAS data of eight haematological malignancies from the FinnGen database were used as the outcome. We performed two-sample Mendelian randomization analysis using inverse-variance weighting (IVW), the weighted median estimator (WME), and MR‒Egger. Sensitivity analysis was also conducted to assess the stability of all MR results. Results Our findings revealed that 57 gut microbiota species may be causally related to haematological malignancies. Specifically, the family Oxalobacteraceae (OR = 1.828, 95% CI = 1.284 to 2.602, p = 0.0008) exhibited a strong positive correlation with the occurrence of ML. Additionally, the presence of the class Erysipelotrichia (OR = 8.738, 95% CI = 2.469 to 30.930, p = 0.001), the order Erysipelotrichales (OR = 8.738, 95% CI = 2.469 to 30.930, p = 0.001), and the family Erysipelotrichaceae (OR = 8.738, 95% CI = 2.469 to 30.930, p = 0.001) exhibited strong positive correlations with the occurrence of MCL. On the other hand, the class Methanobacteria (OR = 0.525, 95% CI = 0.338 to 0.816, p = 0.004), order Methanobacteriales (OR = 0.525, 95% CI = 0.338 to 0.816, p = 0.004), and family Methanobacteriaceae (OR = 0.525, 95% CI = 0.338 to 0.816, p = 0.004), as well as the class Actinobacteria(OR = 0.394, 95% CI: 0.209 to 0.746, p = 0.004), and genus Lachnospiraceae UCG001 (OR = 0.411, 95% CI = 0.232 to 0.728, p = 0.002), exhibited strong negative correlations with the occurrence of mature NK/T-cell lymphoma. These results suggest that the gut microbiota may play a significant role in the development of haematological malignancies. Conclusions Our findings suggest a causal relationship between the gut microbiota and haematological malignancies, which may contribute to providing new insights into the mechanisms of microbiota-mediated hematological malignancies. Additionally, these findings might lead to the identification of risk factors and early prediction of haematological malignancies.

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Exploring the Characteristics of Intestinal Microbiota in Hematologic Malignancy Patients via 16s rDNA High-Throughput Sequencing

Cited by 5 publications

References 0 publications

Treadmill Exercise Modulates Intestinal Microbes and Suppresses LPS Displacement to Alleviate Neuroinflammation in the Brains of APP/PS1 Mice

Treadmill Exercise Modulates Intestinal Microbes and Suppresses LPS Displacement to Alleviate Neuroinflammation in the Brains of APP/PS1 Mice

Alteration of gut microbiome and correlated amino acid metabolism are associated with acute myelocytic leukemia carcinogenesis

Causal Relationship between the Gut Microbiota and Haematological Malignancies: A Two-Sample Mendelian Randomization Analysis

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