Exploring New Antioxidant and Mineral Compounds from Nymphaea alba Wild-Grown in Danube Delta Biosphere

Cudalbeanu, Mihaela; Ghinea, Ioana Otilia; Furdui, Bianca; Dah-Nouvlessounon, Durand; Raclea, Robert-Cristian; Costache, Teodor; Cucolea, Elena Iulia; Urlan, Florentina; Dinică, Rodica Mihaela

doi:10.3390/molecules23061247

Cited by 39 publications

(36 citation statements)

References 24 publications

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Section: Electrochemical Evaluation Of Antioxidant Capacitysupporting

confidence: 67%

“…The λmax of quercetin is at 385 nm [58], and its oxidative degradation exposed to electrochemical measurements is highlighted in Figure 10b. Therefore, the observed electrochemical process supports the relevant antioxidant activity of N. alba extracts, which can be attributed to quercetin [22,58], one of the flavonoids present in the leaf extracts. The presence of quercetin was previously confirmed by HPLC-MS/MS analysis using quercetin as standard [22].…”

Section: Electrochemical Evaluation Of Antioxidant Capacitysupporting

confidence: 67%

“…Therefore, the observed electrochemical process supports the relevant antioxidant activity of N. alba extracts, which can be attributed to quercetin [22,58], one of the flavonoids present in the leaf extracts. The presence of quercetin was previously confirmed by HPLC-MS/MS analysis using quercetin as standard [22]. The identification was performed based on their characteristic [M-H] − and MS/MS fragmentation profile, according to the literature data, the MS spectra providing a parent ion at 301 m/z, and a fragment ion at 150 m/z.…”

Section: Electrochemical Evaluation Of Antioxidant Capacitysupporting

confidence: 67%

“…With all this in mind, we searched for antifungal, antitumoral, and antioxidant properties in extracts of Nymphaea alba (N. alba) (Figure 1), a member of a large Nymphaeaceae family of aquatic plants worldwide distributed and used for hundreds of years in traditional medicine. N. alba species contain antioxidant polyphenols, flavonoids and derived acids such as caffeic acid, ferulic acid, quinic acid, p-coumaric acid, gallic acid, quercetin, luteolin, orientin, catechin, epicatechin, naringin, naringenin and rutin phytochemicals that have shown inhibitory effects on different phases of cell cycle and are also responsible for inducing apoptosis by regulating the expression of different apoptotic signaling pathways [22][23][24]. Flower extracts of the Nymphaea genus have been reported to have antineoplastic effects in different types of cancer, including leukemia, lung cancer, colon cancer and prostate cancer [25].…”

Section: Introductionmentioning

confidence: 99%

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Antifungal, Antitumoral and Antioxidant Potential of the Danube Delta Nymphaea alba Extracts

et al. 2019

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This study aimed to explore for the first time the biological properties such as antifungal, antitumoral and antioxidant of Danube Delta Nymphaea alba (N. alba) leaf and root methanolic extracts. The toxicity studies of N. alba extracts showed no inhibitory effect on wheat seed germination by evaluating the most sensitive physiological parameters (Germination %, Germination index, Vigor index) and using confocal laser scanning microscopy images. The analyzed extracts were found to have high antifungal activity against Candida glabrata with MIC values of 1.717 µg/mL for leaf and 1.935 µg/mL for root. The antitumor activity of the both extracts against A2780/A2780cisR ovarian, LNCaP prostate and MCF-7 breast cancer cells was promising with IC50 values ranging from 23–274 µg/mL for leaf and 18–152 µg/mL for root, and the combination of N. alba extracts with cisplatin showed a synergistic effect (coefficient of drug interaction <1). The antioxidant properties were assessed by β-carotene bleaching, ABTS and FRAP assays and cyclic voltammetry. Quercetin, the most prominent antioxidant, was quantified in very good yields by spectroelectrochemical assay.

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Section: Electrochemical Evaluation Of Antioxidant Capacitysupporting

confidence: 67%

Section: Electrochemical Evaluation Of Antioxidant Capacitysupporting

confidence: 67%

Section: Electrochemical Evaluation Of Antioxidant Capacitysupporting

confidence: 67%

Section: Introductionmentioning

confidence: 99%

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Antifungal, Antitumoral and Antioxidant Potential of the Danube Delta Nymphaea alba Extracts

et al. 2019

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“…Its MS 2 spectrum showed an ion at m/z 417 [(M‐H)‐162] − , which was due to the loss of glucoside. Compound 22 was identified as naringin based on comparison with reported values .…”

Section: Resultsmentioning

confidence: 99%

Identification of flavonoids in Dendrobium huoshanense and comparison with those in allied species of Dendrobium by TLC, HPLC and HPLC coupled with electrospray ionization multi‐stage tandem MS analyses

et al. 2019

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Dendrobium huoshanense, a unique species in the genus Orchidaceae, is only found in China and is known as “mihu”. Due to the lack of quality control, the use of D. huoshanense in the herbal market has been limited. In this study, methods based on thin‐layer chromatography, high‐performance liquid chromatography and high‐performance liquid chromatography coupled with electrospray ionization multi‐stage tandem mass spectrometry were used to identify the flavonoids in D. huoshanense and distinguish this species from other Dendrobium species. Using thin‐layer chromatography, a characteristic band was observed for D. huoshanense, and this band was absent from the thin‐layer chromatography plates of other Dendrobium species. Then, using high‐performance liquid chromatography, nine peaks of flavonoids were observed in the chromatograms of ten batches of D. huoshanense. Ultimately, 22 flavonoids in D. huoshanense were identified by multi‐stage tandem mass spectrometry, and 11 of these compounds are being reported from D. huoshanense for the first time. In addition, two compounds both with molecular weights of 710, were identified as being unique to D. huoshanense; one of these compounds, apigenin‐6‐C‐α‐L‐rhamnosyl‐(1→2)‐β‐D‐glucoside‐8‐C‐α‐L‐arabinoside, was proven to be responsible for the characteristic thin‐layer chromatography band of D. huoshanense. These analysis methods can be applied for the identification and quality control of D. Huoshanense.

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Polyphenol fingerprinting and hypoglycemic attributes of optimized Cycas circinalis leaf extracts

et al. 2020

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BACKGROUND Cycas circinalis leaves are used to treat diabetes mellitus in local medicinal systems without any scientifically proved information on their medicinal potential and phytochemicals. In this study, the total phenolic contents, total flavonoid contents, and 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH) scavenging and inhibitory effects on α‐glucosidase and α‐amylase were determined for optimized hydroethanolic leaf extracts. Secondary metabolites were identified using ultra‐high‐performance liquid chromatography–quadrupole time‐of‐flight mass spectrometry (UHPLC–QTOF‐MS/MS). In vivo studies on diabetic albino mice were also carried out to evaluate the impact of the most active extract on their blood glucose levels. RESULTS The 60% ethanolic extract showed the highest extract yield (209.70 ± 0.20 g kg−1) and total phenolic (154.24 ± 3.28 mg gallic acid equivalent) and flavonoid (78.52 ± 1.65 mg rutin equivalent per gram dried extract) contents and exhibited the maximum DPPH scavenging activity (IC50 = 59.68 ± 2.82 μg mL−1). The IC50 values for inhibition of α‐glucosidase (58.42 ± 2.22 μg mL−1) and α‐amylase (74.11 ± 1.70 μg mL−1) were also significant for the 60% ethanolic extract. The untargeted UHPLC–QTOF‐MS/MS‐based metabolite profiling confirmed the presence of iridoid glucoside, gibberellin A4, O‐β‐d‐glucosyl‐4‐hydroxy‐cinnamate, 3‐methoxy‐2‐phyenyl‐4H‐furo[2,3‐h]chromen‐4‐one, kaempferol, withaferin A, amentoflavone, quercitin‐3‐O‐(6″‐malonyl glucoside), ellagic acid, and gallic acid. Plant extract at a dose of 500 mg kg−1 body weight reduced the blood glucose level by a considerable extent and also improved the lipid profile of diabetic mice after a 28‐day trial. CONCLUSION The findings revealed the medicinal potential of C. circinalis leaves to treat diabetes mellitus and provided the nutraceutical leads for functional food development. © 2020 Society of Chemical Industry

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Exploring New Antioxidant and Mineral Compounds from Nymphaea alba Wild-Grown in Danube Delta Biosphere

Cited by 39 publications

References 24 publications

Antifungal, Antitumoral and Antioxidant Potential of the Danube Delta Nymphaea alba Extracts

Antifungal, Antitumoral and Antioxidant Potential of the Danube Delta Nymphaea alba Extracts

Identification of flavonoids in Dendrobium huoshanense and comparison with those in allied species of Dendrobium by TLC, HPLC and HPLC coupled with electrospray ionization multi‐stage tandem MS analyses

Polyphenol fingerprinting and hypoglycemic attributes of optimized Cycas circinalis leaf extracts

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