Exploring different photosensitizers to optimize elimination of planktonic and biofilm forms of Enterococcus faecalis from infected root canal during antimicrobial photodynamic therapy

Pourhajibagher, Maryam; Kazemian, Hossein; Chiniforush, Nasim; Hosseini, Nava; Pourakbari, Babak; Azizollahi, Atefeh; Rezaei, Fahimeh; Bahador, Abbas

doi:10.1016/j.pdpdt.2018.09.014

Cited by 52 publications

(34 citation statements)

References 26 publications

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“…The number of bacteria was adjusted using a standard curve. For the erythrosine supplement group, 100 μL of erythrosine was added, whereas 100 μL of PBS was added to the control groups [14,15].…”

Section: Bacterial Strainmentioning

confidence: 99%

Application of Teeth Whitening LED for Prevention of Dental Caries : Antimicrobial Photodynamic Therapy Approach

Park¹,

Park²,

Lee³

et al. 2020

THE JOURNAL OF THE KOREAN ACADEMY OF PEDTATRIC DENTISTRY

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The present study is aimed to assess the effect of antimicrobial photodynamic therapy (aPDT) on Streptococcus mutans biofilm through teeth whitening light emitting diode (LED).Planktonic and dynamic biofilm state cultures of S. mutans were used. Erythrosine 20 μM/L was used as the photosensitizer. Irradiation was performed by exposing cultures to clinic and homecare whitening LEDs for 15 minutes. The viability was measured through Colony Forming Unit counts and confocal laser scanning microscopy.aPDT using whitening LEDs and erythrosine significantly decreased the CFU count of S. mutans compared to that in the control group. Dynamic biofilm group showed more resistant features to aPDT compared with planktonic state. Clinic and homecare whitening LED device showed similar antimicrobial effect. The whitening LED, which could irradiate the entire oral arch, showed a significant photodynamic effect on cariogenic S. mutans biofilm. aPDT mediated by erythrosine and LEDs used for teeth whitening exhibited promising antimicrobial activity. Abstract 70Corresponding author : Juhyun Lee

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Section: Bacterial Strainmentioning

confidence: 99%

Application of Teeth Whitening LED for Prevention of Dental Caries : Antimicrobial Photodynamic Therapy Approach

Park¹,

Park²,

Lee³

et al. 2020

THE JOURNAL OF THE KOREAN ACADEMY OF PEDTATRIC DENTISTRY

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“…E. faecalis is one of the most detected microorganisms in persistent apical periodontitis [ 31 ]. This gram-positive facultative anaerobe exists inside the root canal, and is related to periradicular lesions; it can penetrate and colonize dentinal tubules [ 32 ]. For these reasons, E. faecalis was selected in this study to evaluate the antimicrobial activity of the materials tested.…”

Section: Discussionmentioning

confidence: 99%

Comparison of in vitro biocompatibility and antibacterial activity of two calcium silicate-based materials

Liu

Hé

Wang

et al. 2021

J Mater Sci: Mater Med

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This study is aimed at comparing and evaluating the biocompatibility and antibacterial activities of mineral trioxide aggregate (MTA) and iRoot BP Plus as novel retro-filling materials. Discs of both materials were prepared and incubated for 72 h to obtain material extracts in medium. Flow cytometry and the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay were used to assess the rate of apoptosis and proliferation of human periodontal ligament stem cells (hPDLSCs) when exposed to eluates of both materials. The expression levels of alkaline phosphatase, collagen type I, osteocalcin, Runt-related transcription factor-2, and Osterix were tested for evaluating the osteogenic differentiation of hPDLSCs. The antibacterial activities of both materials were compared by the direct contact test. The hPDLSCs stimulated by MTA or iRoot BP Plus eluates showed significantly higher cell viability than that of the control group with no eluates. No significant differences were observed among the percentages of necrotic and apoptotic cells stimulated by MTA and iRoot BP Plus eluates and the control group. The expression of all osteogenic differentiation markers of hPDLSCs in both experimental groups were significantly higher than those of the control group, while the increment values in MTA group were significantly higher than those of the iRoot BP Plus group. The antibacterial activity against Enterococcus faecalis showed no significant difference between MTA and iRoot BP Plus. Therefore, both materials may be suitable for retro-filling applications.

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“…The absorption peak of the PS should be in accordance with the wavelength of the light in order to produce reactive oxygen species (ROS) such as singlet oxygen which induce injury or death to microorganisms. 31,32 The maximum absorption doses of the PSs MB, TB, and Curcumin are 660, 630, and 450 nm respectively, 33 and the wavelength of the LED lamp used in the present study was 630 nm. Evaluating the photochemical and antimicrobial effects of phytotherapeutic PSs, Nardini et al 32 reported that curcumin presented similar ROS production to MB when activated at 660 nm.…”

mentioning

confidence: 83%

“…They reported that CUR-PDT led to the highest bactericidal and anti-biofilm activity against E. faecalis, which was significantly more than that of TBand MB-PDT. 33 This may be due to a different wavelength of LED used for activating curcumin in their study (450 nm) which led to greater absorption of light by this PS.…”

mentioning

confidence: 92%

Comparing the Efficacy of Toluidine Blue, Methylene Blue and Curcumin in Photodynamic Therapy Against Enterococcus faecalis

et al. 2020

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Introduction: Antimicrobial photodynamic therapy (aPDT) as a supplement to the conventional root canal preparation has shown promising results. Previous studies have adopted various combinations of light sources and photosensitizers, which makes it difficult to compare the disinfection efficacy of different PDT protocols. The aim of the present study was to compare the efficacy of three photosensitizers (toluidine blue, methylene blue, and curcumin) in PDT using LED against Enterococcus faecalis in root canal disinfection. Methods: Root canals of 54 single-rooted extracted teeth were prepared using the ProTaper Gold rotary system and were incubated with E. faecalis for three weeks. They were then randomly divided into five experimental groups and a control group: (1) Irrigation with 2.5% NaOCl for 30 seconds, (2) NaOCl irrigation followed by TB-PDT, (3) NaOCl irrigation followed by MB-PDT, (4) NaOCL irrigation followed by curcumin-PDT, (5) Curcumin solvent (1% ethanol+1% BSA), ( 6) Control (irrigation with normal saline). Sampling was done by collecting dentin shavings from the root canals, and colony-forming units were determined for each treatment group. The data were analyzed by Kruskal-Wallis and Mann-Whitney U tests. The significance level was set at P˂0.05. Results: In all treatment groups, the mean values of colony forming unit (CFU) decreased by 99% compared to the control group. The lowest mean values of CFU were observed in groups 2 and 4, followed groups 3, 1, and 5 respectively. The mean CFU count in group 2 was significantly lower than that of group 1 (P value=0.011), while there were no significant differences among groups 1, 3, and 4 (P value >0.05). Conclusion:The adjunction of toluidine blue-mediated PDT by means of a light-emitting diode to NaOCl irrigation increased its antibacterial efficacy against E. faecalis and could be an effective complementary method in root canal disinfection.

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Exploring different photosensitizers to optimize elimination of planktonic and biofilm forms of Enterococcus faecalis from infected root canal during antimicrobial photodynamic therapy

Cited by 52 publications

References 26 publications

Application of Teeth Whitening LED for Prevention of Dental Caries : Antimicrobial Photodynamic Therapy Approach

Application of Teeth Whitening LED for Prevention of Dental Caries : Antimicrobial Photodynamic Therapy Approach

Comparison of in vitro biocompatibility and antibacterial activity of two calcium silicate-based materials

Comparing the Efficacy of Toluidine Blue, Methylene Blue and Curcumin in Photodynamic Therapy Against Enterococcus faecalis

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