Comparison of in vitro biocompatibility and antibacterial activity of two calcium silicate-based materials

Abstract

This study is aimed at comparing and evaluating the biocompatibility and antibacterial activities of mineral trioxide aggregate (MTA) and iRoot BP Plus as novel retro-filling materials. Discs of both materials were prepared and incubated for 72 h to obtain material extracts in medium. Flow cytometry and the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay were used to assess the rate of apoptosis and proliferation of human periodontal ligament stem cells (hPDLSCs) when exposed to eluates of both materials. The expression levels of alkaline phosphatase, collagen type I, osteocalcin, Runt-related transcription factor-2, and Osterix were tested for evaluating the osteogenic differentiation of hPDLSCs. The antibacterial activities of both materials were compared by the direct contact test. The hPDLSCs stimulated by MTA or iRoot BP Plus eluates showed significantly higher cell viability than that of the control group with no eluates. No significant differences were observed among the percentages of necrotic and apoptotic cells stimulated by MTA and iRoot BP Plus eluates and the control group. The expression of all osteogenic differentiation markers of hPDLSCs in both experimental groups were significantly higher than those of the control group, while the increment values in MTA group were significantly higher than those of the iRoot BP Plus group. The antibacterial activity against Enterococcus faecalis showed no significant difference between MTA and iRoot BP Plus. Therefore, both materials may be suitable for retro-filling applications.

Physicochemical Properties, Cytocompatibility, and Biocompatibility of a Bioactive Glass Based Retrograde Filling Material

“…In this regard, we used HCEMs, which are human cementoblast-like cell lines [ 27 ], because the evaluation of the cytocompatibility of materials with cementoblast-like cells is beneficial for clinical applications. Biological tests with inadequate material conditions, such as those related to setting and sterilization, have been conducted in many studies although a freshly mixed material is applied to the root canal in clinical practice and elution from this material affects surrounding periapical tissue [ 42 , 43 ]. As a result, we assessed cytocompatibility and biocompatibility using freshly mixed materials, ensuring that our method is more representative of clinical conditions.…”

The Effect of Ultrasonic Agitation on the Porosity Distribution in Apically Perforated Root Canals Filled with Different Bioceramic Materials and Techniques: A Micro-CT Assessment

“…Although MTA-based materials have been widely used for root perforation repair since their first introduction [ 12 ], various investigations of hydraulic calcium silicate-based cements (HCSC) have shown that BioRoot RCS (Septodont, Saint-Maur-des-Fosses, France) could be effectively used as a filler and seal the apical root perforation as well [ 13 ]. BioRoot RCS possesses all the necessary antibacterial, biocompatible and bioactive properties, which promote the regeneration of periapical tissues and contribute to the recruitment of osteo-odontogenic stem cells within the apical environment [ 14 ].…”

The osteoinductive potential of different root-end filling materials in a rat femur model

“…MTA, accepted as the gold standard as a root-end filling material with its anti-inflammatory and osteogenic potential, as well as many favorable properties, has been evaluated in many studies 32 – 34 . nMTA, which contains tricalcium silicate (alite), dicalcium silicate (belite), calcium sulfate (as anhydrite), and a low amount of calcite that facilitates the formation of calcium hydroxide, has taken its place among the new products as an advanced version of MTA 35 .…”