Exploring Charged Polymeric Cyclodextrins for Biomedical Applications

Abstract

Over the years, cyclodextrin uses have been widely reviewed and their proprieties provide a very attractive approach in different biomedical applications. Cyclodextrins, due to their characteristics, are used to transport drugs and have also been studied as molecular chaperones with potential application in protein misfolding diseases. In this study, we designed cyclodextrin polymers containing different contents of β- or γ-cyclodextrin, and a different number of guanidinium positive charges. This allowed exploration of the influence of the charge in delivering a drug and the effect in the protein anti-aggregant ability. The polymers inhibit Amiloid β peptide aggregation; such an ability is modulated by both the type of CyD cavity and the number of charges. We also explored the effect of the new polymers as drug carriers. We tested the Doxorubicin toxicity in different cell lines, A2780, A549, MDA-MB-231 in the presence of the polymers. Data show that the polymers based on γ-cyclodextrin modified the cytotoxicity of doxorubicin in the A2780 cell line.

Multicharged cyclodextrin supramolecular assemblies

“…Although the literature contains numerous reviews covering the diverse applications of CDs and CD-based assemblies, including metal complexation, 11,41,161,162 cyclodextrin metal–organic frameworks, 163 cyclodextrin covalent organic frameworks, 164–167 supramolecular catalysis and synthesis, 168–170 multistimuli-responsive materials, 15,171–173 polymer materials, 13,24,174–181 self-healing materials, 182 amphiphilic materials, 90,183 crystalline organic materials, 184 liquid crystal materials, 14 rotaxanes/polypseudorotaxanes/catenanes/polyrotaxanes, 185–190 drug/protein/gene delivery, 16,19,23,191–194 molecular recognition and imaging, 1,195–199 molecular machines, 200 thiolated cyclodextrins, 201 cyclodextrin–porphyrinoid systems, 202 foods and antioxidants, 203,204 electrochemical analysis, 5 and chiral analysis, 205 to the best of our knowledge, multicharged CDs as an important building block have not been comprehensively reviewed. Multilevel supramolecular assembly based on electrostatic interactions between opposite charges, including parent CDs modified with multiple charges or encapsulating charged guest molecules, has enabled the construction of a diverse variety of multifunctional materials, and these have been widely applied in drug delivery, 206–214 bioimaging, 215–218 molecular recognition, 219–231 nanochannels, 232,233 molecular switches, 26,234 adsorbents and enrichment, 235–237 surfactants, 238 electrospinning supramolecular systems, 239,240 supercapacitors, 241 CD–polyoxometalate complexes, 242–245 liquid crystal materials, 246 multistimuli-responsive materials, 247–252 pseudorotaxanes, 253 conductive polymers, 254 photodynamic/chemotherapy, 255 molecular shuttles, 256,257 etc.…”

Recombinant therapeutic proteins degradation and overcoming strategies in CHO cells

“…Protein degradation is closely related to the degree of aggregation, and protein stabilizers, such as cyclodextrin added to the medium could reduce aggregation heterogeneity and thus inhibit degradation (Bognanni et al 2021 ). In addition, the addition of ferric citrate to IFN-γ-producing CHO culture significantly inhibited proteolysis, indicating metalloproteinases presence in the culture.…”

Effect of Biomass as Nucleating Agents on Crystallization Behavior of Polylactic Acid

“…It is a slightly conical ring with a hydrophobic inner cavity and hydrophilic outer cavity, which has unique molecular container properties. It is widely used in biomedical fields [ 67 ].…”