Exploring Antioxidant and Enzymes (A-Amylase and B-Glucosidase) Inhibitory Activity of Morinda lucida and Momordica charantia Leaves from Benin

Abstract

Background: Momordica charantia Linn. (Cucurbitaceae), the wild variety of bitter melon and Morinda lucida Benth (Rubiaceae) were commonly used as a popular folk medicine in Benin. This research focused to measure the antioxidant and enzyme inhibitory effects of M. charantia and M. lucida leaves and their antidiabetic activity. Methods: Antioxidant activities were evaluated by micro-dilution technique using DPPH free radical scavenging activity and β-carotene-linoleate bleaching assay. The α-amylase inhibition assay was carried out utilizing the 3,5-dinitrosalicylic acid procedure, while β-glucosidase inhibition assay was demonstrated using as substrate p-nitrophenyl-β-D-glucopyranoside (PNPG). HPLC-DAD analysis was realized using a high-performance liquid chromatography systems with diode-array detector, L-3000. Results: Chlorogenic acid, epicatechin, daidzein, rutin, naringin, quercetin, naringenin and genistein were identified as polyphenol compounds in the both plants extract. Dichloromethane and ethyl acetate extracts showed a good α-amylase inhibitory activity (56.46 ± 1.96% and 58.76 ± 2.74% respectively). M. lucida methanolic extract has shown IC50 of 0.51 ± 0.01 mg/mL, which is the lowest for DPPH scavenging activity. M. lucida dichloromethane extract showed the highest inhibitory capacity of β-glucosidase activity (82.11. ± 2.15%). Conclusion: These results justify some traditional medicinal uses of both plants. The purified fractions could be used in future formulations, possibly incorporated in functional foods to combat certain diseases.

Efficacy of Euphorbia helioscopia in context to a possible connection between antioxidant and antidiabetic activities: a comparative study of different extracts

“…It was evaluated by the methodology as described previously [ 27 ] with a few modifications. Five serial diluted concentrations (0.312 to 5 mg/ml) of the plant extract (500 μl) and 500 μl of porcine pancreatic amylase solution (0.5 mgml − 1 in 0.02 M PBS with 6.9 pH having 0.006 M NaCl) was poured and incubated for 10 min at room temperature.…”

Comparison of biogenic silver nanoparticles formed by Momordica charantia and Psidium guajava leaf extract and antifungal evaluation

“…Indeed, many researches confirmed that the phenolics and saponins (e.g. gallic acid, p-coumaric acid, chlorogenic acid, tannic acid, rutin, naringin, quercetin, epicatechin (-), genistein, naringenin and daidzein) distributed in various M. charantia tissues including leaves [20][21][22]. Diaz-de-Cerio et al studied about polar compounds in guava leaves, they found 13 ellagic and gallic acid derivatives, quercetin and its derivatives, catechin, gallocatechin, gallic acid, naringenin and many guavinosides [17].…”

Flavonoid Profiles of Two New Approved Romanian Ocimum Hybrids

“…The IC 50 values were graphically determined by plotting the inhibition percent against inhibitory concentration. The antioxidant activity index (AAI) was calculated using following formula: [ 23 , 42 ].…”

“…The gradient elution was from 2% to 100% B at 30 °C for 60 min, and the elution flow was set at 1000 µL/min. Further, 300 nm analytical wavelength was used for detection in accordance with the literature [ 42 ].…”