Flavonoid Profiles of Two New Approved Romanian Ocimum Hybrids

Abstract

Basil (Ocimum spp.) is a traditional herbal medicine abundant in antioxidants such as phenolic compounds. As part of a diet, this herb is proved to have some roles in decreasing the risk of cancer, and in the treatment of inflammation and neurodegenerative diseases. This study aims to explore the total phenolic and flavonoid content of two new basil hybrids growing in Romania, namely “Aromat de Buzau” (AB) and “Macedon” (MB). The antioxidant capacity of those two species was also analyzed by DPPH and cyclic voltammetry. Six different flavonoids, such as catechin (+), rutin, hyperoside, naringin, naringenin, and genistein, were separated, identified, and quantified by HPLC–DAD chromatography, for the first time, from romanian basil hybrids. The main flavonoid of the extracts was found to be naringin which is present in the highest amount (26.18 mg/kg) in “Aromat de Buzau” (O. basilicum) methanolic extract. These results suggest that dietary intake of these new hybrids can be a source of antioxidant compounds.

Comparative Study of Natural Antioxidants from Glycine max, Anethum graveolensand Pimpinella anisum Seed and Sprout Extracts Obtained by Ultrasound-Assisted Extraction

“…The determination of the total polyphenol content was performed according to a previously published method, adapted to microplate reader with 96-well plates (Tecan Pro 200, Tecan Trading AG, Männedorf, Switzerland) using Folin-Ciocalteu reagent [41,42]. The Folin-Ciocalteu reagent is a mixture of phosphomolybdic acid (H 3 PMo 12 O 40 ) and phosphotungstic acid (H 3 PW 12 O 40 ) that reacts with phenols and non-phenolic reducing substances to form chromogens which can be detected spectrophotometrically, since in alkaline conditions the oxotungstate and oxomolybdate formed in this redox reaction show a blue coloration proportional to the concentration of polyphenols [43,44]. Briefly, in this method 10 µL of sample were incubated with 25 µL of Folin-Ciocalteu reagent for 5 min, then 25 µL of 20% Na 2 CO 3 and 140 µL of ultrapure water were added and incubated for 30 min at room temperature.…”

Sono-Biosynthesis and Characterization of AuNPs from Danube Delta Nymphaea alba Root Extracts and Their Biological Properties

“…For the calibration curves, concentrations between 10 and 400 µg/mL were used. The total polyphenols were analyzed at 300 nm detection [97][98][99].…”

“…After 30 min of standing at room temperature, the samples were placed in a Tecan Pro 200 multiwell plate reader (Tecan, Männedorf, Switzerland) and their absorbance was recorded at 760 nm. As the standard references, gallic acid (0.97-500 µg/mL) and tannic acid (0.97-500 µg/mL) were used, and the results were expressed as equivalents of gallic acid (mg GAEq/g) and tannic acid (mg TAEq/g) per 1 g of sample [97][98][99].…”

“…AlCl 3 and ultrapure water were used to prepare the blank samples. As the standard references, quercetin (0.45-250 µg/mL) and rutin (0.45-250 µg/mL) were used, and the results were expressed as equivalents of quercetin (mg QEq/g) and rutin (mg REq/g) per 1 g of the sample [97][98][99].…”

Allantoin from Valuable Romanian Animal and Plant Sources with Promising Anti-Inflammatory Activity as a Nutricosmetic Ingredient

“…Over time, different methods have been developed for allantoin dosing: alkaline titration, TLC [29], HPLC or spectrophotometry [5,30]. Nowadays, there is a special interest for the discovery of new antioxidant bioactive compounds including polyphenols [31][32][33][34], polysaccharides [35,36], but also naturally derived peptides [37]. Recently, the antioxidant activity was analyzed on different samples obtained from comfrey roots [5,22,26] and snail mucus [4,17,18].…”