Exploration And Morphology Identification of Spores Asbuscular Mycorrhizal Fungi from Horticultural Plantation

Abstract

Differences in location and rhizosphere cause differences in species diversity and population of Arbuscular Mycorrhizal Fungi (AMF). In addition, not all AMF have the same morphological and physiological characteristics, therefore it is very important to know their identity. This study aims to determine the presence and number of spores as well as to determine the morphological characteristics of AMF originating from the rhizosphere of several horticultural crops in the agricultural land of Payakumbuh State Agricultural Polytechnic. The method used in this research is descriptive exploratory method by means of purposive sampling for soil sampling. While the stages of this research include: taking and collecting data in the field, determining the point of location for soil sampling, taking soil samples, analyzing soil properties in the laboratory, isolating AMF spores and identifying AMF spores morphologically. The conclusions of this study are 1) The population of AMF spores in horticultural land is high. The highest spore population was found in soil samples of the root area of shallot plants (556 spores per 10 g of soil), while the lowest number of spores was in soil samples of eggplant root areas (271 spores per 10 g of soil), 2) AMF exploration in several horticultural crops in the agricultural land of the Payakumbuh State Agricultural Polytechnic, based on morphological identification (shape, color and size), the AMF found consisted of three genera, namely Glomus sp, Gigaspora sp, and Scutelospora sp.

Exploration and isolation of indigenous arbuscular mycorrhizal fungi in post-landslide sandy and clayey silt for eco-friendly remediation

“…They can be hyaline to yellow, reddish-brown, brown, or black in color and range in size from 20 to 400 μm [12]. The spore surface is relatively smooth, with thin spore walls, and the diameter of the Glomus spores found in this study ranged from 38 to 90μm [13].…”

A New Technique for the Extraction of Arbuscular Mycorrhizae Fungal Spores from Rhizosphere

“…In both the WST and the UWST, it is necessary to spend more time extracting fungal spores due to using sieves and centrifugations. In addition, the WST needs at least 10 g of a rhizosphere sample for fungal spore extraction [ 14 , 39 , 40 ]. On the other hand, the UCT allows for the efficient processing of multiple samples of small amounts (1 g) in a relatively short time (around 8 min for a minimum of six samples).…”

Identification of the Diversity of Indigenous Arbuscular Mycorriza Fungi in the Rhizosphere of Coffee (Coffea Sp) Arabica Solok Radjo West Sumatera

“…Various factors can affect the diversity of arbuscular mycorrhizal fungi. For example, plant diversity (Lee et al, 2013;Armansyah et al, 2018;Susila et al, 2022), soil fertility (Montiel et al, 2016, and environmental changes (Hazard et al, 2013). Differences in plant varieties also determine the diversity of arbuscular mycorrhizal fungi (Wagg et al, 2011;Johnson et al, 2015).…”

“…According to the type of AMF spores, there will be different types and numbers in plants, one of which is caused by variety and genetics (Lee et al 2013) (12) Plant types also affect AMF diversity (Armansyah et al, 2018;Susila et al 2022). Coffee varieties will determine AMF diversity in the rhizosphere.…”