Exploiting DELLA Signaling in Cereals

Velde, Karel Van De; Ruelens, Philip; Geuten, Koen; Rohde, Antje; Straeten, Dominique Van Der

doi:10.1016/j.tplants.2017.07.010

Cited by 105 publications

(99 citation statements)

References 83 publications

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“…There are five DELLA family members in Arabidopsis thaliana (Arabidopsis), GA-INSENSITIVE (GAI), REPRESSOR OF GA (RGA), RGA-LIKE1 (RGL1), RGL2 and RGL3 (reviewed by Claeys et al, 2014;Nelson & Steber, 2016). DELLA proteins modulate target genes expression in three ways: preventing transcription factor (TF) from binding its target genes, promoting transcriptional activity of TF or sequestrating transcriptional regulator to regulate TF activity (reviewed by Thomas et al, 2016;Van de Velde et al, 2017). DELLA proteins modulate target genes expression in three ways: preventing transcription factor (TF) from binding its target genes, promoting transcriptional activity of TF or sequestrating transcriptional regulator to regulate TF activity (reviewed by Thomas et al, 2016;Van de Velde et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Interplay between REVEILLE1 and RGA‐LIKE2 regulates seed dormancy and germination in Arabidopsis

et al. 2019

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Environmental light signal and GAs synergistically regulate seed dormancy and germination. The phytochrome B (phyB) photoreceptor regulates expression of the REVEILLE1 (RVE1) transcription factor, which directly inhibits GIBBERELLIN 3-OXIDASE2 transcription, suppressing GA biosynthesis. However, whether phyB-RVE1 coordinates with GA signaling in controlling seed dormancy and germination remains unknown.Here, we demonstrate that RVE1 regulation of seed dormancy and germination requires a DELLA repressor, REPRESSOR OF GA-LIKE2 (RGL2), in Arabidopsis thaliana.RVE1 interacts with both RGL2 and its E3 ubiquitin ligase SLEEPY1 (SLY1) and promotes RGL2 stability by restraining the RGL2-SLY1 interaction. Furthermore, RVE1 and RGL2 synergistically regulate global transcriptome changes; RGL2 enhances the DNA-binding capacity and transcriptional activity of RVE1 in regulating downstream gene expression. Moreover, RGL2 expression is repressed by phyB.Our study reveals a novel regulatory mechanism in which the RVE1-RGL2 module coordinately controls seed dormancy and germination by integrating light perception, GA metabolism and GA signaling pathways.

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Section: Introductionmentioning

confidence: 99%

Interplay between REVEILLE1 and RGA‐LIKE2 regulates seed dormancy and germination in Arabidopsis

et al. 2019

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“…DELLA proteins, master repressors of the GA signaling pathway, participate in diverse physiological processes by interacting with many different transcription factors including BZR1, BES1, PIFs, MYC2, JAZ1, EIN3/EIL1, ARR1, ARF6, TCP14, ALC, SCL3, SPL9, etc. (Van De Velde et al ). Recent studies showed that overexpression of the GA catabolic gene GA2ox led to an increased number of tillers or branches in Rice, Tomato, Turfgrass, and Populus trees, suggesting that GA negatively regulates the formation of axillary buds (Agharkar et al ; Lo et al ; Mauriat et al ; Martinez‐Bello et al ), but little is known about the mechanisms involved.…”

Section: Introductionmentioning

confidence: 99%

Gibberellin repression of axillary bud formation in Arabidopsis by modulation of DELLA‐SPL9 complex activity

Zhang

Wang

et al. 2019

JIPB

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The formation of lateral branches has an important and fundamental contribution to the remarkable developmental plasticity of plants, which allows plants to alter their architecture to adapt to the challenging environment conditions. The Gibberellin (GA) phytohormones have been known to regulate the outgrowth of axillary meristems (AMs), but the specific molecular mechanisms remain unclear. Here we show that DELLA proteins regulate axillary bud formation by interacting and regulating the DNA‐binding ability of SQUAMOSA‐PROMOTER BINDING PROTEIN LIKE 9 (SPL9), a microRNA156‐targeted squamosa promoter binding protein‐like transcription factor. SPL9 participates in the initial regulation of axillary buds by repressing the expression of LATERAL SUPPRESSOR (LAS), a key regulator in the initiation of AMs, and LAS contributes to the specific expression pattern of the GA deactivation enzyme GA2ox4, which is specifically expressed in the axils of leaves to form a low‐GA cell niche in this anatomical region. Nevertheless, increasing GA levels in leaf axils by ectopically expressing the GA‐biosynthesis enzyme GA20ox2 significantly impaired axillary meristem initiation. Our study demonstrates that DELLA‐SPL9‐LAS‐GA2ox4 defines a core feedback regulatory module that spatially pattern GA content in the leaf axil and precisely control the axillary bud formation in different spatial and temporal.

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“…DELLA proteins control several plant processes through interaction with a myriad of transcriptional factors (Van De Velde et al, 2017). By affecting protein-protein interaction, SLR1 SUMOylation has the potential to modulate plant processes by altering the transcriptional outcome of SLR1-TFs interacting proteins.…”

Section: Discussionmentioning

confidence: 99%

“…In Arabidopsis, DELLA interaction with GAI-ASSOCIATED FACTOR1 (GAF1) TF triggers a negative-feedback loop that activates GA biosynthesis and subsequent DELLA degradation (Fukazawa et al, 2017). The increasing number of validated DELLA interactors showcase an overlap with most hormone pathways, impacting development, fertility, and seed number (Davière and Achard, 2015; Van De Velde et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

“…DELLA activity is mostly modulated through post-translational modifications (PTMs), which impact its ability to interact with transcriptional regulators and modulate gene expression (Van De Velde et al, 2017). Besides ubiquitination and subsequent proteasome-mediated degradation, there are other PTMs that influence the activity of the DELLA’s in response to GA signalling.…”

Section: Introductionmentioning

confidence: 99%

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SUMOylation of rice DELLA SLR1 modulates transcriptional responses and improves yield under salt stress

Gonçalves¹,

Fernandes²,

Nunes

et al. 2020

Preprint

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Author Contributions: 15 NMG participated in the conception of research, executed most experiments, and 16 wrote the article with contributions from all authors. TF did protein interaction 17 experiments. MTGR characterized SUMO-protease rice lines. CCM analysed data 18 and completed the writing. MAAR purified recombinant proteins. PMB analysed RNA 19 seq. MMO supervised experiments and analysed results. IAA conceived original 20 project, supervised experiments, and finalised the article. 31 32 Short title (50 characters): SUMOylation of rice SLR1 increases salt tolerance 33 34 ABSTRACT 35 DELLA proteins modulate GA signalling and are major regulators of plant plasticity to 36 endure stress. DELLAs are mostly regulated at the post-translational level, and their 37 activity relies on the interaction with upstream regulators and transcription factors 38 (TFs). SUMOylation is a post-translational modification (PTM) capable of changing 39protein interaction and found to influence DELLA activity in Arabidopsis. We 40 determined that SUMOylation of the single rice DELLA SLENDER RICE1 (SLR1) 41 occurs in a lysine residue different from the one identified in Arabidopsis 42 REPRESSOR OF GA (RGA), which suggests differential DELLA regulation by 43SUMOylation in monocots and dicots. Remarkably, artificially increasing 44 SUMOylated SLR1 (SUMO1SLR1) levels attenuated the penalty of salt stress on 45 plant yield. Gene expression analysis revealed that the overexpression of 46 SUMOylated SLR1 regulates key dioxygenases that modulate active GA levels, 47namely GA20ox2 and GA2ox3, which could partially explain the sustained 48 productivity upon salt stress imposition. Besides, SLR1 SUMOylation blocked the 49 interaction with the growth regulator YAB4, which may fine-tune GA20ox2 50 expression. Mechanistically, we propose that SLR1 SUMOylation disrupts the 51 interaction with members of several transcription factor families to modulate gene 52 expression. We found that SLR1 SUMOylation represents a novel mechanism 53 modulating DELLA activity, which attenuates the impact of stress on plant 54 performance. 55 56 gibberellin 58 59 One sentence summary: Rice plants show increased yield under salt stress when 60 its gibberellin transcriptional regulator DELLA protein is artificially SUMOylated.

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Exploiting DELLA Signaling in Cereals

Cited by 105 publications

References 83 publications

Interplay between REVEILLE1 and RGA‐LIKE2 regulates seed dormancy and germination in Arabidopsis

Interplay between REVEILLE1 and RGA‐LIKE2 regulates seed dormancy and germination in Arabidopsis

Gibberellin repression of axillary bud formation in Arabidopsis by modulation of DELLA‐SPL9 complex activity

SUMOylation of rice DELLA SLR1 modulates transcriptional responses and improves yield under salt stress

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