Interplay between REVEILLE1 and RGA‐LIKE2 regulates seed dormancy and germination in <i>Arabidopsis</i>

Yang, Liwen; Jiang, Zhimin; Liu, Shuangrong; Lin, Rongcheng

doi:10.1111/nph.16236

Cited by 41 publications

(42 citation statements)

References 60 publications

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“…Furthermore, RVE1 stabilizes a DELLA protein RGA-LIKE2 (RGL2) to depress seed germination and enhance seed dormancy. Meanwhile, RGL2 could enhance the transcriptional activity of RVE1 during seed dormancy and germination (Yang et al 2020). As described above, we summarize that PIF1 and RVE1 play key roles in light-mediated seed germination, and that they could be regulated by other factors.…”

Section: Introductionsupporting

confidence: 57%

“…Similarly, RVE1 enhances the DNA-binding capacity (and may enhance transcriptional activity) on ABI3 expression by PIF1 ( Figure 5). We previously showed that RGL2 also accelerates the DNA-binding ability and transcriptional activity of RVE1 in regulating GA3ox2 expression (Yang et al 2020). LEUNIG_HOMOLOG interacts with PIF1 and functions as a transcriptional coregulator to inhibit seed germination (Lee et al 2015).…”

Section: Discussionmentioning

confidence: 99%

“…In addition, PIF1 directly regulates GA and ABA signaling through modulating the transcription of target genes, such as RGA, GAI, and ABI3 (Oh et al 2007(Oh et al , 2009. RVE1 not only regulates GA3ox2 expression, but also promotes RGL2 protein stability (Jiang et al 2016;Yang et al 2020). Here, we demonstrate that PIF1 and RVE1 synergistically control the expression of a set of genes involved in GA metabolism, ABA biosynthesis, ABA signaling, and the transcription factor/regulators under phyB-on and/or phyB-off conditions (Figure 4).…”

Section: Discussionmentioning

confidence: 99%

“…Seed germination assay was performed as described (Yang et al 2020). Plants of different genotypes were grown in the same batch under long-day (16 h light/8 h dark) at 22 ± 1°C.…”

Section: Seed Germination Assaymentioning

confidence: 99%

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PIF1 and RVE1 form a transcriptional feedback loop to control light‐mediated seed germination in Arabidopsis

Yang

Jiang

Jing

et al. 2020

JIPB

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The phytochrome B (phyB) photoreceptor plays a major role that inputs light signals to regulate seed dormancy and germination. PHYTOCHROME‐INTERACTING FACTOR1 (PIF1) is a key transcription factor repressing phyB‐mediated seed germination, while REVEILLE1 (RVE1) factor functions as a curial regulator in controlling both seed dormancy and germination. However, the relationship between the PIF1‐ and RVE1‐modulated signaling pathways remains mostly unknown. Here, we find that PIF1 physically interacts with RVE1. Genetic analysis indicates that RVE1 inhibition on seed germination requires PIF1; reciprocally, the repressive effect of PIF1 is partially dependent on RVE1. Strikingly, PIF1 and RVE1 directly bind to the promoter and activate the expression of each other. Furthermore, PIF1 and RVE1 coordinately regulate the transcription of many downstream genes involved in abscisic acid and gibberellin pathways. Moreover, PIF1 enhances the DNA‐binding ability and transcriptional repression activity of RVE1 in regulating GIBBERELLIN 3‐OXIDASE2, and RVE1 promotes PIF1's DNA‐binding ability in modulating ABSCISIC ACID‐INSENSITIVE3 expression. Thus, this study demonstrates that PIF1 and RVE1 form a transcriptional feedback loop that coordinately inhibits seed germination, providing a mechanistic understanding of how phyB‐mediated light signal is transduced to the seeds.

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Section: Introductionsupporting

confidence: 57%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

“…Seed germination assay was performed as described (Yang et al 2020). Plants of different genotypes were grown in the same batch under long-day (16 h light/8 h dark) at 22 ± 1°C.…”

Section: Seed Germination Assaymentioning

confidence: 99%

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PIF1 and RVE1 form a transcriptional feedback loop to control light‐mediated seed germination in Arabidopsis

Yang

Jiang

Jing

et al. 2020

JIPB

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“…ABI4 is a key transcription factor that can be directly or indirectly involved in the regulation of key genes in the ABA and GA biogenesis pathways leading to seed dormancy [9]. The transcription factor REVEILLE1 (RVE1) interacts with REPRESSOR OF GA-LIKE2 (RGL2) to regulate the dormancy and germination of Arabidopsis thaliana seeds by integrating light perception, GA metabolism and the associated signalling pathways [10]. In the process of GAmediated promotion of seed germination, GA3ox1 and GA3ox2 are two key genes regulating GA synthesis [11], while GA2ox promotes GA degradation [12].…”

Section: Introductionmentioning

confidence: 99%

A Combined Analysis of the Transcriptome and Metabolome Revealed the Molecular Mechanism by which GA3 Disrupts Dormancy in Leymus Chinensis Seeds

Bing¹,

Zhang²,

Wang³

et al. 2020

Preprint

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Background: Leymus chinensis is a perennial forage grass that has good palatability, high yield and high feed value, but seed dormancy is a major problem limiting the widespread cultivation of L. chinensis. The aim of this study was to investigate the underlying molecular mechanism and identify candidate genes associated with dormancy disruption by gibberellic acid (GA3) through transcriptomic and metabolomic analysis.Results: The germination test revealed that the optimum concentration of GA3 for disruption of L. chinensis seed dormancy was 200 μg/L. Compared with seeds soaked in sterile water, a total of 4,327 and 11,919 differentially expressed genes (DEGs) and 871 and 650 differentially abundant metabolites were identified in de-hulled and hulled seeds treated with GA3, respectively. Most of the DEGs were associated with starch and sucrose metabolism, protein processing in the endoplasmic reticulum, endocytosis and ribosomes. Furthermore, isoquinoline alkaloid biosynthesis, tyrosine metabolism, starch and sucrose metabolism, arginine and proline metabolism, and amino sugar and nucleotide sugar metabolism were significantly enriched pathways. Integrative analysis of the transcriptomic and metabolomic data revealed that starch and sucrose metabolism is one of the most important pathways that may play a key role in the energy supply for the transition of L. chinensis seeds from a dormant state to germination by suppressing the expression of Cel61a, egID, cel1, tpsA, SPAC2E11.16c and TPP2, along with enhancing the expression of AMY1.1, AMY1.2, AMY1.6 and GLIP5, and finally inhibiting the synthesis of cellobiose, cellodextrin, and trehalose while promoting the hydrolysis of sucrose, starch, cellobiose, cellodextrin, and trehalose to glucose.Conclusions: This study identified several key genes and provided new insights into the molecular mechanism of seed dormancy release by GA3 in L. chinensis. These putative genes will be valuable resources for improving the seed germination rate in future breeding studies.

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Trichomics: Trichomes as Natural Chemical Factories

Dhankhar

Regmi

Kawatra

et al. 2022

Phytochemical Genomics

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Interplay between REVEILLE1 and RGA‐LIKE2 regulates seed dormancy and germination in Arabidopsis

Cited by 41 publications

References 60 publications

PIF1 and RVE1 form a transcriptional feedback loop to control light‐mediated seed germination in Arabidopsis

PIF1 and RVE1 form a transcriptional feedback loop to control light‐mediated seed germination in Arabidopsis

A Combined Analysis of the Transcriptome and Metabolome Revealed the Molecular Mechanism by which GA3 Disrupts Dormancy in Leymus Chinensis Seeds

Trichomics: Trichomes as Natural Chemical Factories

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