Exploitation of Dunaliella for β-carotene production

Raja, Rathinam; Hemaiswarya, Shanmugam; Rengasamy, Ramasamy

doi:10.1007/s00253-006-0777-8

Cited by 231 publications

(107 citation statements)

References 63 publications

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“…As is known, the halotolerant marine microalga Dunaliella, especially D. salina and D. bardawil, accumulates massive b-carotene under stress conditions (Raja et al 2007;Ye et al 2008). Molecular identification of the carotenogenesis in Dunaliella would effectively promote the production of b-carotene and other carotenoids by biological methods.…”

Section: Discussionmentioning

confidence: 99%

Structural and phylogenetic analysis of a novel ζ‐carotene desaturase from Dunaliella bardawil, a unicellular alga that accumulates large amounts of β‐carotene

Liu

Jiang

2010

Limnology & Oceanography

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Due to the high demand for carotenoids in the global market, more and more attention is being paid to the molecular elucidation of carotenogenesis in Dunaliella for the massive production of b-carotene. The complementary deoxyribonucleic acid (cDNA) of a novel carotenogenic enzyme, f-carotene desaturase (Zds), was isolated from Dunaliella bardawil using polymerase chain reaction. The full-length cDNA was 2209 base pairs (bp) long, containing an 1749-bp putative open reading frame that encoded a deduced protein sequence of 582 amino acids with an estimated molecular weight of 64.429 kDa. The D. bardawil Zds was a nonsecretory protein that may be located in the chloroplast. The D. bardawil Zds had a closer relationship with chlorophyte and higher plants Zdses than with those of other species in the phylogenetic analysis. The three-dimensional structure of the D. bardawil Zds is predicted.

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Section: Discussionmentioning

confidence: 99%

Structural and phylogenetic analysis of a novel ζ‐carotene desaturase from Dunaliella bardawil, a unicellular alga that accumulates large amounts of β‐carotene

Liu

Jiang

2010

Limnology & Oceanography

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“…O potencial comercial das microalgas para a produção industrial de alimentos funcionais a partir destes metabólitos é amplamente reconhecido. Consequentemente, o cultivo em massa de microalgas, visando o aumento da produção desses compostos bioquímicos, essenciais para a saúde humana, é de grande interesse prático (Inbaraj et al, 2006;Raja et al, 2007).…”

Section: Introductionunclassified

INFLUÊNCIA DA LUMINOSIDADE E CONCENTRAÇÃO SALINA NA PRODUÇÃO DE LIPÍDIOS E CAROTENOIDES PELA MICROALGA Dunaliella tertiolecta EM FOTOBIORREATOR AIRLIFT

Fré¹,

Rech²,

Marcílio³

2015

Anais Do XX Congresso Brasileiro De Engenharia Química

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RESUMO -A microalga Dunaliella tertiolecta é dos poucos microrganismos fotossintéticos capaz de produzir simultaneamente grandes quantidades de carotenoides e lipídios em determinadas condições de estresse ambientais, como luminosidade e concentração salina do meio de cultivo. Porém estas condições normalmente diminuem a velocidade de crescimento das microalgas. Neste trabalho foi investigado o efeito do aumento da luminosidade e da concentração salina durante os cultivos a fim de promover a maior síntese destes bioprodutos. Para isso, os cultivos foram realizados em duas fases: (1) produção de grande número de células nas condições ideais de crescimento e, posteriormente, (2) aplicação de condições de estresse salino e luminoso. Os melhores resultados foram a 30 klx, obtendo-se 1,24 ± 0,06 g L -1 de biomassa, 5,08 ± 0,04 mg L -1 de carotenoides no meio de cultivo, correspondendo a uma concentração 4,34 ± 0,38 mg g -1 de biomassa seca, e 10,21 ± 0,03 % de lipídios na biomassa, em 210 h de cultivo.

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“…Dunaliella salina is the best known commercial source of natural β-carotene among all organisms in the world (Borowitzka 1995). This alga was shown to accumulate β-carotene to more than 10 % of its dry weight when grown under growth-limiting conditions such as high irradiance, high salinity, high temperature, and limiting nutrients (Ben-Amotz et al 1982;Ben-Amotz & Avron 1983;Cowan et al 1992;Salguero et al 2003;Raja et al 2007;Einali & Valizadeh 2015). β-carotene accumulates as droplets in intrachloroplastic lipoidal globules and plays a role in protecting chlorophyll against photo-damage (Ben-Amotz & Avron 1983;Ben-Amotz et al 1988).…”

Section: Introductionmentioning

confidence: 99%

Metabolic responses and β-carotene production by the unicellular green alga Dunaliella salina exposed to leaf extracts

et al. 2017

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Th e present work investigated the eff ects of aqueous extracts of eucalyptus (Eucalyptus globulus) and elderberry (Sambucus ebulus) leaves on β-carotene productivity in Dunaliella salina, a green microalga. Leaf extracts from eucalyptus have greater amounts of phenolics and fl avonoids, as well as greater ferric reducing antioxidant potential than elderberry. Th e extracts of both species greatly inhibited growth of algal suspensions. However, chlorophyll and β-carotene concentration increased in cells treated with leaf extracts, and the highest values were detected in 1 % eucalyptus and 2 % elderberry extracts. Fresh weight, total sugar, and protein content signifi cantly increased following exposure of cells to diff erent doses of leaf extracts. However, in doses containing more than 2 % eucalyptus, the upward trend for total sugar and protein ceased and remained statistically unchanged. Th ese results suggest that metabolic modifi cations enable D. salina cells to tolerate the stress induced by the leaf extracts through allocating carbon fl ux to the synthesis of osmolytes and putative antioxidant molecules (e.g. sugars and β-carotene). Th erefore, the use of leaf extracts holds potential to be a promising and eff ective way to improve D. salina cultivation for β-carotene production and other biotechnological and industrial applications.

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Exploitation of Dunaliella for β-carotene production

Cited by 231 publications

References 63 publications

Structural and phylogenetic analysis of a novel ζ‐carotene desaturase from Dunaliella bardawil, a unicellular alga that accumulates large amounts of β‐carotene

Structural and phylogenetic analysis of a novel ζ‐carotene desaturase from Dunaliella bardawil, a unicellular alga that accumulates large amounts of β‐carotene

INFLUÊNCIA DA LUMINOSIDADE E CONCENTRAÇÃO SALINA NA PRODUÇÃO DE LIPÍDIOS E CAROTENOIDES PELA MICROALGA Dunaliella tertiolecta EM FOTOBIORREATOR AIRLIFT

Metabolic responses and β-carotene production by the unicellular green alga Dunaliella salina exposed to leaf extracts

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