Explant Orientation and Polarity Determine the Morphogenic Response of Epicotyl Segments of Troyer Citrange

García-Luis, Amparo; Bordón, Y.; Moreira-Dias, J.M.; Molina, Rosa Victoria; Guardiola, J. L.

doi:10.1006/anbo.1999.0972

Cited by 52 publications

(36 citation statements)

References 15 publications

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“…Considering BAP culture media supplementation, contrasting with other citrus cultivars (GARCÍA-LUIS et al, 1999;MOREIRA-DIAS et al, 2001;MOURA et al, 2001) the presence of cytokinin is not essential for adventitious bud development. The percentage of responsive explants was stimulated with BAP concentrations up to 0.55 mg L -1 (Figure 2).…”

Section: Resultsmentioning

confidence: 96%

In vitro organogenesis of rangpur lime

et al. 2012

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-Rangpur lime (Citrus limonia Osbeck) in vitro organogenesis was studied based on explant type and cytokinin culture media supplementation. Four explants types collected from epicotyl or hypocotyl regions of in vitro germinated seedlings were evaluated. The epicotyl-derived explants consisted of epicotyl segments and the hypocotyl-derived explants consisted of the entire hypocotyl segment, the hypocotyl segment attached to a cotyledon fragment, and the hypocotyl segment divided longitudinally. The explants were cultured on EME culture medium supplemented with benzylaminopurine (0, 0.5, 1.0, or 1.5 mg L -1 ). The evaluation was performed after 6 weeks. Best results considering both the explant responsiveness and number of shoots developed per explants were obtained when epicotyl segments-derived explants were evaluated. Considering the explant responsiveness of hypocotyl segments-derived explants no difference was detected between the entire hypocotyl segment and the hypocotyl segment attached to a cotyledon fragment. Moreover, the percentage of responsive explants decreased when hypocotyl segments divided longitudinally were tested. No difference was detected for the number of shoots developed per explant considering the three types of hypocotyl-derived explants. Culture media supplementation with BAP was not essential for Rangpur lime in vitro organogenesis. However, adventitious shoot development was stimulated in concentrations between 0.5 -1.0 mg L -1 . Index terms: adventitious shoot; Citrus limonia; tissue culture. ORGANOGÊNESE IN VITRO DE LIMÃO CRAVORESUMO -A organogênese in vitro de limão Cravo (Citrus limonia Osbeck) foi estudada em função do tipo de explante e da suplementação do meio de cultura com citocinina. Foram avaliados quatro tipos de explantes coletados da região do epicótilo e do hipocótilo de plântulas germinadas in vitro. Os explantes consistiram em segmentos de epicótilo, segmentos de hipocótilo, segmentos de hipocótilo ligado a um fragmento do cotilédone e segmentos de hipocótilo cortado longitudinalmente. Os explantes foram cultivados em meio de cultura EME suplementado com benzilaminopurina (0; 0,5; 1,0 e 1,5 mg L -1 ). As avaliações foram realizadas após seis semanas de incubação, e os melhores resultados considerando-se tanto a porcentagem de explantes responsivos como o número de gemas desenvolvidas por explante foram obtidos com o uso de segmentos de epicótilo como explante. Considerando os explantes derivados da região do hipocótilo, não foi detectada diferença na porcentagem de explantes responsivos com o uso de segmentos de hipocótilo ou de segmentos de hipocótilo ligado a um fragmento do cotilédone. A porcentagem de explantes responsivos diminuiu com o uso de segmentos de hipocótilo cortado longitudinalmente como explante. Não foi detectada nenhuma diferença no número de gemas adventícias desenvolvidas por explante considerando-se os três tipos de explantes derivados da região do hipocótilo. A suplementação do meio de cultura com BAP não é essencial para a organogênese ...

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Section: Resultsmentioning

confidence: 96%

In vitro organogenesis of rangpur lime

et al. 2012

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“…In Citrus, the morphogenic response of in vitro cultures depends on genotype, explant type and orientation, composition of culture medium, and conditions of incubation (Durán-Vila et al, 1989;Ghorbel et al, 1998;García-Luis et al, 1999;Bordon et al, 2000;Costa et al, 2004). Epicotyl segments from in vitro germinated seedlings have a higher morphogenic ability than other tissues used as explant source.…”

Section: Parâmetros De Cultura De Tecidos Em Cultivares De Laranjeiramentioning

confidence: 99%

Tissue culture parameters in sweet orange cultivars

Mendes

Cidade

Manzoli

et al. 2008

Pesq. agropec. bras.

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-The objective of this work was to establish tissue culture parameters for gene transfer in sweet orange cultivars. Epicotyl explants with different ages were cultured with 6-benzylaminopurine (BAP), kanamycin and hygromycin. Shoots were cultured with alpha-naphthaleneacetic acid (NAA) alone or in combination with indole-3-butyric acid (IBA). The requirement of BAP for shoot development was genotype-specifi c. Epicotyl explants from 35-day-old seedlings produced signifi cantly more shoots per explant in 'Pêra'. Kanamycin inhibited shoot regeneration for the most cultivars. The percentage of shoots that produced roots in 'Pêra' was signifi cantly higher in medium with NAA and IBA than with NAA alone.

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“…More recently, efforts have been made to define efficient protocols for the recovery of plants through organogenesis of citrus genotypes (Peña et al 1995, Cervera et al 1998, García-Luis et al 1999, Almeida et al 2003a and related species (Hassanein andAzooz 2003/4, Hiregoudar et al 2005).…”

Section: Introductionmentioning

confidence: 98%

Histological characterization of in vitro adventitious organogenesis in Citrus sinensis

Almeida¹,

Filho²,

Mendes³

et al. 2006

Biol. plant.

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The adventitious bud development was induced in epicotyl segments of Valencia sweet orange (Citrus sinensis L. Osbeck). Seeds were cultured in vitro for three weeks in the dark, followed by one week at a 16-h photoperiod. Epicotyl segments were cultured horizontally for the induction of organogenesis in Murashige and Tucker (1969, MT) culture medium supplemented with 1.0 mg dm -3 benzylaminopurine. Samples were observed by light and scanning electron microscopy from day zero to day 25, when buds were well grown. It was shown that the adventitious buds originated directly from the cambial region on the cut ends of the explants.

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Explant Orientation and Polarity Determine the Morphogenic Response of Epicotyl Segments of Troyer Citrange

Cited by 52 publications

References 15 publications

In vitro organogenesis of rangpur lime

In vitro organogenesis of rangpur lime

Tissue culture parameters in sweet orange cultivars

Histological characterization of in vitro adventitious organogenesis in Citrus sinensis

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