Histological characterization of in vitro adventitious organogenesis in Citrus sinensis

Almeida, Weliton Antônio Bastos de; Filho, Fernando Aguiar; Mendes, Beatriz Madalena Januzzi; Rodriguez, Adriana Pinheiro Martinelli

doi:10.1007/s10535-006-0044-y

Cited by 17 publications

(10 citation statements)

References 11 publications

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“…This result can be explained with the presence of predetermined cells which first dedifferentiate themselves, subsequently form new meristematic centres, and finally differentiate again producing new organs [34][35][36][37][38][39]. This process, due to cell totipotency, has been observed in several species [30,[40][41][42][43][44][45][46][47] and is very efficient for regeneration. Our results showed that AFLP is a very sensitive and reliable molecular marker technique for revealing specific genomic alterations induced by tissue culture and for identifying slightly different genotypes.…”

Section: Discussionmentioning

confidence: 99%

An evaluation of a new approach to the regeneration of Helichrysum italicum (Roth) G. Don, and the molecular characterization of the variation among sets of differently derived regenerants

Perrini

Alba

Ruta

et al. 2009

Cellular and Molecular Biology Letters

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A protocol for the induction of regeneration from leaves of Helichrysum italicum was established. Calli were found to form on the basal medium only when it was supplemented with thidiazuron (TDZ) alone or in combination with naphthalene acetic acid (NAA), with a percentage ranking of at least 80%. The hormone-free medium showed the highest percentage of shoot regeneration (62%) even though no callus formed. AFLP markers were employed to verify tissue culture-induced variation in the regenerated plantlets obtained by direct shoot regeneration or the indirect shoot regeneration process (callus formation). Seven out of the eleven AFLP primer pairs yielded polymorphic patterns. The average number of fragments per primer pair was 64.1. Singletons were represented by 12 (2.7%) fragments. Student’s T-test was performed both on the average number of shared fragments and on the nucleotide diversity, and no significant statistical difference was observed between the two regeneration treatments.

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Section: Discussionmentioning

confidence: 99%

An evaluation of a new approach to the regeneration of Helichrysum italicum (Roth) G. Don, and the molecular characterization of the variation among sets of differently derived regenerants

Perrini

Alba

Ruta

et al. 2009

Cellular and Molecular Biology Letters

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“…Similar to this research, explants with more differentiated tissues than meristems like scutellum of embryo were used to induce direct somatic embryogenesis pathway in seven cereal species (Eudes et al 2003). It has been proven that globular somatic embryos originate from the epidermal and subepidermal cells of the explant (Laparra et al 1997;Quiroz-Figueroa et al 2002;Petitprez et al 2005), while shoot organogenesis derives from meristematic shoot initials at different anatomical layers (Zhang et al 1998;de Almeida et al 2006;Veit 2006). Co-induction of direct shoot organogenesis and direct somatic embryogenesis pathways on different parts of a single explant, in response to the same hormonal cues, clearly shows the vital effect of the site in response to hormone.…”

Section: Discussionmentioning

confidence: 99%

Induction and comparison of different in vitro morphogenesis pathways using embryo of cumin (Cuminum cyminum L.) as a model material

Ebrahimie

Naghavi

Hosseinzadeh

et al. 2007

Plant Cell Tiss Organ Cult

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In this study, using cumin embryo as explant and manipulating plant growth regulators (PGRs) in regeneration medium, the main in vitro morphogenesis pathways including direct shoot organogenesis, direct somatic embryogenesis, indirect somatic embryogenesis, and indirect shoot organogenesis were obtained. The effects of PGRs, subculture, and light on the induction and progression of different pathways were studied in detail. Direct shoot organogenesis occurred on the meristematic zone, while direct somatic embryogenesis was observed on hypocotyl part of cumin embryo (more differentiated part). Application of BAP (0.1 mgl À1 ) was the sole triggering factor for induction of callus and indirect regeneration pathways. Exogenous IAA played the central role in the direct somatic embryogenesis pathway; however, the combined effects of IAA and NAA along with the high endogenous cytokinin level resulted in direct shoot organogenesis. Subculturing revealed accelerating effects on direct somatic embryogenesis pathway and callus formation. Conversely, subculturing had negative effect on direct shoot organogenesis pathway. In certain combinations of PGRs, like 0.4 mgl À1 IAA + 0.4 mgl À1 NAA, co-induction and co-regeneration of different pathways were observed. Investigation of genotype dependencies of different pathways showed that direct pathways are more genotype-dependent, stable, and faster than indirect pathways. This research presents the embryo of cumin as a convenient model material for induction and comparison of different morphogenesis pathways.

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“…As also mentioned by Almeida, Mourao Filho, Mendes, & Rodríguez (2006), a direct regeneration pathway may be used for transformation purposes as a means to avoid the formation of chimeric plants and genetic variability, which can occur through regeneration from callus. This protocol may be a useful tool for micropropagation of the species as well as for the application of genetic transformation techniques, as it enables us to determine specific regions in the foliar explant where initiation of meristemoids will take place, and therefore to determine which cells should be the object of genetic transformation.…”

Section: Discussionmentioning

confidence: 99%

Histología de la regeneración por organogénesis en Paulownia tomentosa (Paulowniaceae)

San-José

Cernadas

Corredoira

2014

RBT

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Paulownia tomentosa is a fast-growing tree species with a considerable economic potential because of its value for wood as well as its high biomass production, and elevated stress tolerance. The objective of the present study was to evaluate the development of adventitious buds in leaves obtained from four-week-old shoots of P. tomentosa, in order to identify the cells involved in in vitro adventitious bud development. Leaves (proximal halves with the petiole) from the first node were excised from four-week-old micropropagated shoots, and cultured on Murashige and Skoog medium, supplemented with 3% (w/v) sucrose, 0.6% (w/v) Sigma agar, 22.7µM thidiazuron (TDZ) and 2.9µM indole-3-acetic acid for two weeks, explants were then transferred to the same medium with 0.44µM N 6 -benzyladenine for another four weeks. Five explants were collected daily during the two first weeks in TDZ treatment. A total of 140 samples were processed. Most of the buds developed indirectly from the callus formed in the petiole stub, and they became visible after eight-ten days of culture, although some buds were also observed in the area of the laminar cut at the level of the veins. The first histological changes could be observed after two-three days of culture, with the dedifferentiation of some subepidermal and inner parenchyma cells, which exhibited a large, prominent nucleus, densely-stained cytoplasm and a high nucleusto-cell area ratio. Proliferation of these cells gives rise to meristemoid formation after seven-ten days of culture. Organized cell division in meristemoids allows the formation of bud primordia that emerged from the explants surface. The progressive structural differentiation of the apical meristem, leaf primordia, and procambium strands, led to formation of complete buds that were observed in the exterior of the explants after 10-15 days of culture. Direct development of buds from cells in the subepidermic and/or epidermic layers were observed on the adaxial surface of the petiole. This protocol may be a useful tool for the application of genetic transformation techniques, as it enables to determine specific regions in the foliar explants where the meristemoids formation will take place, and therefore to determine which cells should be the object of genetic transformation. Rev. Biol. Trop. 62 (2): 809-818. Epub 2014 June 01.Key words: bud induction, histological analysis, organogenesis, Paulownia tomentosa, TDZ.The search for new sources of energy is currently of great interest. One potentially promising option is the use of plant biomass or production of biofuels like bioethanol and biodiesel, which are renewable and do not contribute to climate change (Mandpe, Kadlaskar, Degen, & Keppeler, 2005). Oil-based feedstock or biodiesel can be produced from vegetable oils obtained from agricultural plants such as rapeseed, sunflower, soybean, oil palm and groundnut (Johnson, Eswaran, & Sujatha, 2011). Bioethanol, with features similar to gasoline, is obtained from the fermentation of sugars from sugar cane, beet ...

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Histological characterization of in vitro adventitious organogenesis in Citrus sinensis

Cited by 17 publications

References 11 publications

An evaluation of a new approach to the regeneration of Helichrysum italicum (Roth) G. Don, and the molecular characterization of the variation among sets of differently derived regenerants

An evaluation of a new approach to the regeneration of Helichrysum italicum (Roth) G. Don, and the molecular characterization of the variation among sets of differently derived regenerants

Induction and comparison of different in vitro morphogenesis pathways using embryo of cumin (Cuminum cyminum L.) as a model material

Histología de la regeneración por organogénesis en Paulownia tomentosa (Paulowniaceae)

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