Experimental Studies to Estimate the Intestinal Viability in a Rat Strangulated Ileus Model Using a Dielectric Parameter

Matsuo, Hiroshi; Mori, Yoshio; Takagi, Hisato; Iwata, Hiroji; Yamada, Takuya; Sakamoto, Kenichi; Yasumura, Mikio

doi:10.1023/b:ddas.0000026310.71607.4c

Cited by 8 publications

(12 citation statements)

References 20 publications

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“…Matsuo et al investigated the viability of strangulated rat small intestine (ileum) invivo, using a coaxial 2-electrode probe on the serosa (Matsuo et al, 2004) introducing the use of the tan δ parameter. Radhakrishnan et al measured in-vivo with two small wire electrodes on the surface of rat intestine and found that intestinal edema led to decreasing impedance (Radhakrishnan et al, 2007).…”

Section: Previous Work With Bioimpedance and The Small Intestinementioning

confidence: 99%

In vivocharacterization of ischemic small intestine using bioimpedance measurements

et al. 2016

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The standard clinical method for the assessment of viability in ischemic small intestine is still visual inspection and palpation. This method is non-specific and unreliable, and requires a high level of clinical experience. Consequently, viable tissue might be removed, or irreversibly damaged tissue might be left in the body, which may both slow down patient recovery. Impedance spectroscopy has been used to measure changes in electrical parameters during ischemia in various tissues. The physical changes in the tissue at the cellular and structural levels after the onset of ischemia lead to time-variant changes in the electrical properties. We aimed to investigate the use of bioimpedance measurement to assess if the tissue is ischemic, and to assess the ischemic time duration. Measurements were performed on pigs (n = 7) using a novel two-electrode setup, with a Solartron 1260/1294 impedance gain-phase analyser. After induction of anaesthesia, an ischemic model with warm, full mesenteric arterial and venous occlusion on 30 cm of the jejunum was implemented. Electrodes were placed on the serosal surface of the ischemic jejunum, applying a constant voltage, and measuring the resulting electrical admittance. As a control, measurements were done on a fully perfused part of the jejunum in the same porcine model. The changes in tan δ (dielectric parameter), measured within a 6 h period of warm, full mesenteric occlusion ischemia in seven pigs, correlates with the onset and duration of ischemia. Tan δ measured in the ischemic part of the jejunum differed significantly from the control tissue, allowing us to determine if the tissue was ischemic or not (P < 0.0001, F = (1,75.13) 188.19). We also found that we could use tan δ to predict ischemic duration. This opens up the possibility of real-time monitoring and assessment of the presence and duration of small intestinal ischemia.

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Section: Previous Work With Bioimpedance and The Small Intestinementioning

confidence: 99%

In vivocharacterization of ischemic small intestine using bioimpedance measurements

et al. 2016

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“…Matsuo et al [79] evaluated the viability of a strangulated intestine by measuring its electrical properties in an experimental study. Correlation of changes in dielectric parameters with intestinal ischemia was found.…”

Section: Assessment Of Electrical Propertiesmentioning

confidence: 99%

How to assess intestinal viability during surgery: A review of techniques

Urbanavičius

Pattyn²,

Putte³

et al. 2011

WJGS

188

156

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Objective and quantitative intraoperative methods of bowel viability assessment are essential in gastrointestinal surgery. Exact determination of the borderline of the viable bowel with the help of an objective test could result in a decrease of postoperative ischemic complications. An accurate, reproducible and cost effective method is desirable in every operating theater dealing with abdominal operations. Numerous techniques assessing various parameters of intestinal viability are described by the studies. However, there is no consensus about their clinical use. To evaluate the available methods, a systematic search of the English literature was performed. Virtues and drawbacks of the techniques and possibilities of clinical application are reviewed. Valuable parameters related to postoperative intestinal anastomotic or stoma complications are analyzed. Important issues in the measurement and interpretation of bowel viability are discussed. To date, only a few methods are applicable in surgical practice.Further studies are needed to determine the limiting values of intestinal tissue oxygenation and flow indicative of ischemic complications and to standardize the methods.

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“…We used permittivity measurements to investigate the dielectric properties of healthy, ischemic, and reperfused small intestine in a porcine model. The dielectric properties of biological substances reflect the tissue structures and chemical composition, and both will change during ischemia and reperfusion injury 10 , 12 , 13 . We investigated how the dielectric properties changed with different degrees of ischemia and reperfusion over time.…”

Section: Introductionmentioning

confidence: 99%

Small intestinal viability assessment using dielectric relaxation spectroscopy and deep learning

Hou

Strand-Amundsen

Tronstad

et al. 2022

Sci Rep

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Intestinal ischemia is a serious condition where the surgeon often has to make important but difficult decisions regarding resections and resection margins. Previous studies have shown that 3 h (hours) of warm full ischemia of the small bowel followed by reperfusion appears to be the upper limit for viability in the porcine mesenteric ischemia model. However, the critical transition between 3 to 4 h of ischemic injury can be nearly impossible to distinguish intraoperatively based on standard clinical methods. In this study, permittivity data from porcine intestine was used to analyze the characteristics of various degrees of ischemia/reperfusion injury. Our results show that dielectric relaxation spectroscopy can be used to assess intestinal viability. The dielectric constant and conductivity showed clear differences between healthy, ischemic and reperfused intestinal segments. This indicates that dielectric parameters can be used to characterize different intestinal conditions. In addition, machine learning models were employed to classify viable and non-viable segments based on frequency dependent dielectric properties of the intestinal tissue, providing a method for fast and accurate intraoperative surgical decision-making. An average classification accuracy of 98.7% was obtained using only permittivity data measured during ischemia, and 96.2% was obtained with data measured during reperfusion. The proposed approach allows the surgeon to get accurate evaluation from the trained machine learning model by performing one single measurement on an intestinal segment where the viability state is questionable.

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Experimental Studies to Estimate the Intestinal Viability in a Rat Strangulated Ileus Model Using a Dielectric Parameter

Cited by 8 publications

References 20 publications

In vivocharacterization of ischemic small intestine using bioimpedance measurements

In vivocharacterization of ischemic small intestine using bioimpedance measurements

How to assess intestinal viability during surgery: A review of techniques

Small intestinal viability assessment using dielectric relaxation spectroscopy and deep learning

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