Abstract. In gastrointestinal surgery, surgeon subjectively judges if the organ is healthy from the color. However it is difficult to discriminate a small difference of organ's color by visual inspection. In this paper, we focus on the tissue oxygen saturation (StO 2 ) that represents balance of oxygen demand and supply in tissue and try to estimate its value by transmitted light intensity analysis. We developed a system for measurement of transmitted light intensity using a compact spectrometer and a halogen light source and collected transmitted light intensity data from pig's small intestines. Absorbance of the tissue was then calculated from those data. On the basis of Beer-Lambert law, we estimated StO 2 from the calculated absorbance. Results of evaluation experiment to pig's small intestines suggested the possibility of quantitative evaluation of tissue viability by the proposed method.