Experimental periodontal disease treatment by subgingival irrigation with tetracycline hydrochloride in rats

Abstract

ObjectiveThe aim of this study was to compare subgingival irrigation with tetracycline hydrochloride (TTC-HCL) as adjunctive treatment to scaling and root planning (SRP) on induced periodontitis in rats. Material and methodsIn 60 rats, periodontal disease was ligature-induced at the mandibular left first molar. After 7 days, the ligature was removed and all animals were submitted to SRP, and divided into 2 groups according to the following treatment: C (n=30) - subgingival irrigation with 1 mL of saline; T (n=30) - subgingival irrigation with 1 mL of TTC-HCL (50 mg/mL). Ten animals in each group were euthanized at 7, 15 and 30 days posttreatment. The histometric values were statistically analyzed (p<0.05). ResultsIn the histometric analysis, at 7, 15 and 30 days, Group T (0.72±0.05 mm2, 0.57±0.14 mm2, 0.62±0.07 mm2), showed less bone loss (p<0.05) than Group C (1.35±0.25 mm2; 1.40±0.31 mm2; 1.29±0.27 mm2), respectively. ConclusionsSubgingival irrigation with TTC-HCL was an effective adjunctive treatment for periodontal disease induced in rats.

Therapeutic Effects of Systemic Vitamin K2 and Vitamin D3 on Gingival Inflammation and Alveolar Bone in Rats With Experimentally Induced Periodontitis

“…A curette was used in the buccal and lingual surfaces in a disto‐mesial direction and in furcation and interproximal areas in an apico‐coronal direction 10 times with a pull stroke 21 . Periodontal pockets were irrigated with 1 mL saline solution after SRP 22 …”

Effectiveness of sub gingival irrigation of an indigenous 1% curcumin solution on clinical and microbiological parameters in chronic periodontitis patients: A pilot randomized clinical trial

“…Sub-gingival delivery of antimicrobial agents was shown to be potent both as in office procedures as well as home oral hygiene regime. Various anti bacterial agents such as chlorhexidine (CHX),[123] metronidazole,[4] tetracyclines,[5] and povidone-iodine[6] and herbal products such as propolis[7] and dentol,[8] have been tried in the recent past and were proven to be effective as sub-gingival irrigants in the management of shallow periodontal pockets. However, research still progresses toward identification of drugs, which are more efficient than those currently used.…”

Effect of andiroba oil on periodontitis in Wistar rats

“…In several studies with similar methodology 6,12,13,18 , but with different substances testing, was found decreased inflammatory response but was not shown difference on the measurement from the cemento-enamel junction, this fact probably occurs because the short time of analysis after application of substances, which is usually after seven days. Thus, further researches are necessary to observe how much time is appropriate to manage the substances for the recovery of bone loss.…”

“…Because of it, researches were conducted in an attempt to improve the clinical management of this condition and is much used the administer of local and systemic antibiotics to control bacteria present on the periondotitis 12,13 . However the antibiotics must be properly used to avoid the selection of new resistant bacteria.…”