Abstract:PURPOSE:To develop an experimental model to study and radiologically monitor displacement of skin flaps in the pericranium of rats subjected to traction and surgical fixation using suture anchored in a skull bone tunnel or with N-butyl-2-cyanoacrylate (Histoacryl TM ) surgical adhesive.
METHODS:Radiological markers were placed in the subcutis of Wistar rats undergoing subperiosteal detachment of the pericranium with pulling and fixation of the flap. We performed radiography on postoperative days 3, 7, 14, 21, … Show more
“…In our study, we emphasized the importance of developing an experimental model for the study of soft tissue fixation methods and their tissue reactions. We found that the adhesive n-butyl-2-cyanoacrylate is more reactive than suture anchored in a skull bone tunnel; thus, adhesive promotes increased migration of PMN cells and fibroblasts corresponding to the higher repositioning of the flaps in the long term 18 . We also observed during the collection of specimens for histological analysis the presence of cover slip adhesive polymerized between the soft tissue and the bone plate.…”
Section: Discussionmentioning
confidence: 83%
“…This was shown in the treated groups, in which the deposition of type I collagen was higher than that in the control group on postoperative day 7. Although the degree of collagen deposition was similar among the three groups at the end of the experiment, when the flap displacement was analyzed with a radiological control, we found the need to use soft tissue fixation methods to maintain the proposed tissue repositioning 18 .…”
Section: Discussionmentioning
confidence: 85%
“…Traction and fixation of soft tissue flaps in facial plastic surgery is performed by means of soft tissue fixation methods, the most common being sutures, screws (metallic or absorbable), and surgical adhesives [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20] .…”
PURPOSE:To study the repair of pericranium-cutaneous flaps fixed with suture anchored in a skull bone tunnel or N-butyl-2-cyanoacrylate adhesive in Wistar rats with emphasis on the cellular inflammatory response and the production of types I and III collagen.
METHODS:The operated region in the cephalic region of Wistar rats was removed minutes before euthanasia, fixed in formalin, and subjected to histological preparation. Slides were stained with hematoxylin-eosin and Picrosirius. Standardized counts of polymorphonuclear and mononuclear cells, fibroblasts, and macrophages were performed, and the percentages of types I and III collagen were determined. Data collection occurred on days 3, 7, 14, 21, and 45 postoperatively. A value of p<0.05 was considered statistically significant.
RESULTS:Quantitative analysis of the data showed more fibroblasts in the surgical adhesive group than in the nylon monofilament thread groups (p=0.0211). Qualitative analysis showed higher reactivity in the adhesive group, with a predominance of polymorphonuclear cells from days 3−45 and macrophages from days 3−7. The amount of type I collagen exceeded 80% in the treated and control groups at the end of the experiment.
CONCLUSIONS:Subperiosteal detachment triggers a cellular inflammatory response that is amplified using soft tissue fixation methods.The adhesive n-butyl-2-cyanoacrylate was more reactive than the nylon monofilament thread anchored in the skull bone tunnel.
“…In our study, we emphasized the importance of developing an experimental model for the study of soft tissue fixation methods and their tissue reactions. We found that the adhesive n-butyl-2-cyanoacrylate is more reactive than suture anchored in a skull bone tunnel; thus, adhesive promotes increased migration of PMN cells and fibroblasts corresponding to the higher repositioning of the flaps in the long term 18 . We also observed during the collection of specimens for histological analysis the presence of cover slip adhesive polymerized between the soft tissue and the bone plate.…”
Section: Discussionmentioning
confidence: 83%
“…This was shown in the treated groups, in which the deposition of type I collagen was higher than that in the control group on postoperative day 7. Although the degree of collagen deposition was similar among the three groups at the end of the experiment, when the flap displacement was analyzed with a radiological control, we found the need to use soft tissue fixation methods to maintain the proposed tissue repositioning 18 .…”
Section: Discussionmentioning
confidence: 85%
“…Traction and fixation of soft tissue flaps in facial plastic surgery is performed by means of soft tissue fixation methods, the most common being sutures, screws (metallic or absorbable), and surgical adhesives [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20] .…”
PURPOSE:To study the repair of pericranium-cutaneous flaps fixed with suture anchored in a skull bone tunnel or N-butyl-2-cyanoacrylate adhesive in Wistar rats with emphasis on the cellular inflammatory response and the production of types I and III collagen.
METHODS:The operated region in the cephalic region of Wistar rats was removed minutes before euthanasia, fixed in formalin, and subjected to histological preparation. Slides were stained with hematoxylin-eosin and Picrosirius. Standardized counts of polymorphonuclear and mononuclear cells, fibroblasts, and macrophages were performed, and the percentages of types I and III collagen were determined. Data collection occurred on days 3, 7, 14, 21, and 45 postoperatively. A value of p<0.05 was considered statistically significant.
RESULTS:Quantitative analysis of the data showed more fibroblasts in the surgical adhesive group than in the nylon monofilament thread groups (p=0.0211). Qualitative analysis showed higher reactivity in the adhesive group, with a predominance of polymorphonuclear cells from days 3−45 and macrophages from days 3−7. The amount of type I collagen exceeded 80% in the treated and control groups at the end of the experiment.
CONCLUSIONS:Subperiosteal detachment triggers a cellular inflammatory response that is amplified using soft tissue fixation methods.The adhesive n-butyl-2-cyanoacrylate was more reactive than the nylon monofilament thread anchored in the skull bone tunnel.
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