Experimental infection of the bovine udder with Campylobacter coli/jejuni

Lander, K.P.; Gill, KP

doi:10.1017/s0022172400026954

Cited by 36 publications

(20 citation statements)

References 5 publications

(6 reference statements)

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“…The majority of microorganisms are introduced to milk from the contaminated external surface of the teats, equipment or hands of workers (Ayres et al 1980), although mastitis is also mentioned as the possible contamination source. Lander and Gill (1980) induced experimental infection of the bovine udder with Campylobacter coli/jejuni with the subsequent development of a mastitis, and the excretion of this bacteria in milk for several days. The mastitis caused by genus Campylobacter bacteria may be severe, acute with loss of appetite and high fever (Gadmundson and Chirino-Trejo 1993).…”

Section: Discussionmentioning

confidence: 99%

Prevalence and antimicrobial resistance of Campylobacter in raw milk in the selected areas of Poland

Wysok

Wiszniewska-Łaszczych

Uradziński

et al. 2011

Polish Journal of Veterinary Sciences

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During the recent years, an immense increase in the number of food poisoning cases in people caused by Campylobacter (C.) species has occurred. Raw milk, next to poultry meat, is considered the most frequent cause of food poisoning in people caused by the subject bacteria, although it is not always possible to isolate Campylobacter cells from the incriminated milk. Most probably this difficulty is caused by low concentration of the pathogen in milk at the level of 2/3 cells/ml although even such low concentration represents risk to human health. The present study was aimed at determining the occurence of Campylobacter bacteria in milk originating from selected regions of Poland. The isolation method applied in this work was effective in recovering as few as 0.1 cell of Campylobacter per g of food. Among 150 bulk milk samples tested, Campylobacter spp. was isolated from 7 (4.6%) ones. The biochemical identification of the isolated strains conducted by means of conventional biochemical tests as well as by applying the API -Campy tests revealed that all the isolates belonged to the C. jejuni species. Determination of resistance to antibiotics was performed by means of the diffusion disks method for the following antibiotics: gentamicin, ciprofloxacin, ampicillin, chloramphenicol, erythromycin, doxycyclin and tetracycline. Among 7 isolates tested, all were susceptible to ampicillin, chloramphenicol, erythromycin and gentamicin, 28.5% to doxycyclin and 14.2% to tetracycline and ciprofloxacin.

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Section: Discussionmentioning

confidence: 99%

Prevalence and antimicrobial resistance of Campylobacter in raw milk in the selected areas of Poland

Wysok

Wiszniewska-Łaszczych

Uradziński

et al. 2011

Polish Journal of Veterinary Sciences

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“…The route by which C. jejuni enters milk has been much debated and two hypotheses have been presented: milk may be contaminated by means of a naturally occurring campylobacter mastitis, or it may be contaminated with bovine faeces during or after milking. Both are theoretically possible because C. jejuni is commonly present in cattle faeces (Robinson & Jones, 1981) and a campylobacter mastitis has been produced experimentally by Lander & Gill (1980). We have attempted to establish which of these hypotheses is more probable by conducting two surveys.…”

Section: Introductionmentioning

confidence: 99%

A search for the source ofCampylobacter jejuniin milk

Waterman

Park

Bramley³

1984

J. Hyg.

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SUMMARYSamples of milk from 1501 cows with mastitis were negative for Campylobacter jejuni. The faeces of 74 healthy Friesian cows were screened for C. jejuni: 13 % of the samples were positive during the summer when the cows were on pasture, and 51 % were positive in the winter when the cows were housed. Positive samples contained on average 1 x 104 campylobacters per g faeces.It is concluded that faecal contamination rather than udder infection is the means by which campylobacters enter milk and thereby infect man.

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“…A strain of C. jejuni designated V2 1 2X isolated from the milk of a cow which had been experimentally infected with a human isolate (Lander & Gill, 1980) was grown under microaerobic conditions (see Media Section) in nutrient broth containing 7 % sterile horse blood at 42 'C for 18 h. Standardization of inoculum was obtained by a total count of formalinized organisms (red cells removed by differential centrifugation) using a Helber slide under dark-ground illumination. Colony counts on the inoculum were used to confirm numbers of viable organisms administered.…”

Section: Campylobacter Culturementioning

confidence: 99%

Experimental infection of Rhesus monkeys with a human strain ofCampylobacter jejuni

Fitzgeorge

Baskerville

Lander

1981

J. Hyg.

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SUMMARYYoung Rhesus monkeys (Macac mulatta) were infected orally with a human strain of Campylobacter jejuni. The disease induced was mild, with inappetence and diarrhoea of short duration, but prolonged intermittent excretion of the bacteria in the faeces occurred. Bacteraemia was generally present for 2-3 days and later the organisms localized in the liver and gall bladder. Recovered animals, when challenged with the same strain, showed no clinical symptoms, no bacteraemia, and excreted the organisms in the faeces for only 3 days.

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Experimental infection of the bovine udder with Campylobacter coli/jejuni

Cited by 36 publications

References 5 publications

Prevalence and antimicrobial resistance of Campylobacter in raw milk in the selected areas of Poland

Prevalence and antimicrobial resistance of Campylobacter in raw milk in the selected areas of Poland

A search for the source ofCampylobacter jejuniin milk

Experimental infection of Rhesus monkeys with a human strain ofCampylobacter jejuni

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