A search for the source of<i>Campylobacter jejuni</i>in milk

Waterman, Sally C.; Park, R. W. A.; Bramley, A. J.

doi:10.1017/s0022172400064871

Cited by 37 publications

(25 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Up to 6% of bulked raw milk samples in the United Kingdom yield campylobacters (43). Cross-contamination of milk occurs from feces during milking or from Campylobacter mastitis (44,45), and failed pasteurization also has been associated with out-breaks of infection (41,46,47). Studies have shown very high rates of Campylobacter contamination among supermarket chickens (48).…”

Section: Reservoirs and Transmission Of Infectionmentioning

confidence: 99%

Enteric Campylobacter: Purging Its Secrets?

et al. 2004

View full text Add to dashboard Cite

Section: Reservoirs and Transmission Of Infectionmentioning

confidence: 99%

Enteric Campylobacter: Purging Its Secrets?

et al. 2004

View full text Add to dashboard Cite

“…fetus (Devlin & McIntyre, 1983;Harvey & Greenwood, 1983;Klein et al 1986), C. cinaedi and C. fennelliae (Totten et al 1985) may also be involved in enteritis. C. jejuni has been isolated from a wide range of animal species (Luechtefeld, Cambre & Wang, 1981;Rosef et al 1983;Fricker & Metcalfe, 1984;Waterman, Park & Bramley, 1984) and water (Knill, Suckling & Pearson, 1982;Bolton, Coates & Hutchinson, 1985). Unpasteurized cows' milk (Hutchinson et al 280 S. M. ARIMI, C. R. FRICKER AND R. W. A.…”

Section: Introductionmentioning

confidence: 99%

Occurrence of ‘thermophilic’ campylobacters in sewage and their removal by treatment processes

1988

Self Cite

View full text Add to dashboard Cite

SUMMARYRemoval of thermophilic campylobacters from sewage at three different stages of treatment at a trickling filter sewage works has been assessed. Samples of incoming sewage, primary sedimentation effluent and final effluent were taken daily from 06.00 h to 20.00 h for 5 consecutive days and the numbers of campylobacters determined by using a most probable number method. Each sample was cultured using 2 h pre-enrichment followed by enrichment in Preston broth for 48 h and detection by plating. Over 78 % of the incoming campylobacters were removed after primary sedimentation and < 0-1 % remained in the final effluent. Campylobacter jejuni biotype I and biotype II constituted 81-5 % and 15-9 % respectively of the 232 isolates tested. Serotypes common in sewage were common in human faeces. It appears that the trickling filter sewage works removes most of the campylobacters entering the sewage works, but large numbers, estimated to be approximately 1010, are released into the environment daily from a local sewage works.

show abstract

“…Contamination of milk can be caused by direct excretion from an asymptomatic cow with mastitis (17) or, more commonly, by fecal contamination during milking (43). The majority of outbreaks have occurred after the consumption of raw milk (44), although improperly pasteurized milk (1) and postpasteurization contamination have been implicated as sources of outbreaks.…”

mentioning

confidence: 99%

Utility of Multilocus Sequence Typing as an Epidemiological Tool for Investigation of Outbreaks of Gastroenteritis Caused by Campylobacter jejuni

2003

View full text Add to dashboard Cite

Multilocus sequence typing (MLST) has been proven useful for the study of the global population structure of Campylobacter jejuni; however, its usefulness for the investigation of outbreaks of disease caused by C. jejuni has not been proven. In this study, MLST plus sequencing of the flaA short variable region (SVR) were applied to 47 isolates from 12 outbreaks of C. jejuni infection whose relatedness has been determined previously, and the results were compared to those of serotyping and pulsed-field gel electrophoresis (PFGE). Isolates implicated in an outbreak were indistinguishable by all four subtyping methods, with sporadic isolates being distinguished from outbreak isolates. Two sporadic isolates from one outbreak were resistant to SmaI digestion and therefore nontypeable by PFGE but were differentiated from the outbreak strain by the other methods. PFGE and flaA SVR typing were the most discriminatory methods, with discriminatory indices (DI) of 0.930 and 0.923, respectively. However, an epidemic strain from one outbreak was distinguished from the other outbreak isolates by flaA SVR typing; its flaA allele was different at five nucleotides, suggesting that this change was possibly mediated by recombination. MLST was less discriminatory than PFGE and flaA SVR typing (DI ‫؍‬ 0.859), and many of the epidemic strains possessed common sequence types (STs) including ST-8, -21, -22, and -42. However, further discrimination within STs was achieved by flaA SVR typing or PFGE. The results from this study demonstrate that a combined approach of MLST plus flaA SVR typing provides a level of discrimination equivalent to PFGE for outbreak investigations.

show abstract

A search for the source ofCampylobacter jejuniin milk

Cited by 37 publications

References 8 publications

Enteric Campylobacter: Purging Its Secrets?

Enteric Campylobacter: Purging Its Secrets?

Occurrence of ‘thermophilic’ campylobacters in sewage and their removal by treatment processes

Utility of Multilocus Sequence Typing as an Epidemiological Tool for Investigation of Outbreaks of Gastroenteritis Caused by Campylobacter jejuni

Contact Info

Product

Resources

About