Experimental canine model for sentinel lymph node biopsy in the vulva using technetium and patent blue dye

Aquino, José Ulcijara; Pinheiro, Luiz Gonzaga Porto; Vasques, Paulo Henrique Diógenes; Rocha, João Ivo Xavier; Cruz, Diego Alves; Beserra, Hugo Enrique Orsini; Cavalcante, Raíssa Vasconcelos

doi:10.1590/s0102-86502012000200002

Cited by 7 publications

(3 citation statements)

References 17 publications

(20 reference statements)

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“…From the first studies conducted by [17], which showed the method's efficiency for detecting mammary lymph nodes in female dogs, the technique has been used experimentally in canines, albeit timidly, in other anatomical locations, such as the vulva and the stomach [18] [19].…”

Section: Considerationsmentioning

confidence: 99%

Metastasis of Mammary Carcinoma in Bitches: Evaluation of the Sentinel Lymph Node Technique

Beserra¹,

Grandi²,

Düfloth³

et al. 2016

ABCR

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In recent years the development of extremely accurate techniques for the removal of lymph nodes has been seen, such as the Sentinel Lymph Node Biopsy (SLNB) technique. The technique is based on the staggered progression occurring in the lymphatic drainage of neoplasias, and is currently the main prognostic and diagnostic factor in women suffering from mammary carcinoma. This research aims at assessing the sentinel lymph node biopsy accuracy in female dogs suffering from mammary carcinoma. Forty-one dogs with cytological diagnosis of the tumor were used in the research. After injecting a Patent Blue V dye, mastectomy was conducted jointly with lymphadenectomy (for both sentinel and non-sentinel lymph nodes). The material was then serially sectioned and microscopically evaluated by routine H&E stain and immunohistochemical study with the markers for pancytokeratin AE1/AE3 and myoepithelial cells CK14. The study revealed that 46.3% (19/41) of the animals presented nodal metastasis. The sensitivity and specificity values found for SLNB were respectively 89.5% and 100%, with a kappa coefficient of 0.90 and p < 0.0001. Therefore, the SLNB technique offers high sensitivity in the early detection of metastases, allowing higher precision in the staging of oncological patients and, therefore, offering higher chances of survival.

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Section: Considerationsmentioning

confidence: 99%

Metastasis of Mammary Carcinoma in Bitches: Evaluation of the Sentinel Lymph Node Technique

Beserra¹,

Grandi²,

Düfloth³

et al. 2016

ABCR

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“…An easy, convenient, safe, and low-cost technique for intraoperative identification of SLNs consists of the use of vital dyes (11,12). This technique shows high sensitivity and specificity and is primarily used in women.…”

Section: Introductionmentioning

confidence: 99%

Comparison of surgical resection of Axillary Lymph Nodes in Dogs with Mammary Gland Tumors with or without sentinel lymph node visualization with patent blue dye

Souza,

Flecher,

Arrais

et al. 2023

Front. Vet. Sci.

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Introduction:Dogs’ axillary lymph node (ALN) is often difficult to locate before surgical resection. The anatomical location of ALN often discourages Veterinarians from surgical lymphadenectomy. Considering the limited literature available, the actual incidence of metastases and the prognostic relevance are poorly understood.Methods:A non-randomized, prospective clinical study was conducted with female dogs (n = 41) with mammary gland tumor (MGT) in the thoracic or cranial abdominal mammary glands. The study investigated the risks of ALN metastasis based on tumors clinical findings, tumor size, histopathological diagnosis and grade. The main aim of this study was to compare ALN resection with or without patent blue 2.5% (PB) dye injection for sentinel lymph node visualization. A total of 46 mastectomies were performed and five animals underwent two mastectomies. In the first group, 17 patients underwent a mastectomy and lymphadenectomy without PB injection (G1). In contrast, in the second group, 24 patients also received PB injections for sentinel lymph node mapping (G2). The ALN was identified in 38/46 cases (82%). The ALN was identified and excised in only 58% of surgeries in G1(19/46), while in group 2, the lymph node was identified in 92% of the cases and resected in 100% of the cases. The use of PB improves ALN’s identification and reduces the surgical resection time in dogs with MGT.Results and discussion:Surgical time differed between the two groups, as it was significantly shorter in the PB injection group compared to group 1 (80 vs. 45 min) (p < 0.0001). The overall frequency of ALN metastasis was 32%. Macroscopic abnormalities in the lymph nodes, tumor size (>3 cm), and diagnosis of anaplastic carcinoma or grade II/III mammary gland tumors were associated with a higher probability of ALN metastasis. Metastases in the ALNs are more common, in dogs presenting with tumors larger than 3 cm and diagnosed with aggressive histological subtypes. The ALNs should be removed for correct staging, prognostic evaluation, and decision for adjuvant therapy.

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“…This dye is widely used for human lymphatic mapping, for research, and for clinical purposes (Aquino et al 2012, Viehl et al 2013.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of physiological parameters of dogs submitted to uterine lymphatic mapping using patent blue V dye for anaphylaxis detection

et al. 2016

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RESUMO.-[Avaliação de parâmetros fisiológicos de cães submetidos ao mapeamento linfático uterino usando o corante azul patente V para a detecção de anafilaxia.] O mapeamento linfático tem sido realizado em humanos e cães. Embora inúmeros casos de anafilaxia já tenham sido relatados em humanos, não existem tais relatos em cães. O objetivo deste estudo foi identificar a ocorrência de reações adversas ao corante azul patente V em cadelas submetidas ao mapeamento linfático uterino, usando avaliações cardiovasculares e hematológicas. O experimento foi realizado em 14 cadelas mestiças, divididas igualmente em dois grupos (PBV-mapeamento linfático uterino e OEH; Controle -apenas OEH). Os animais foram submetidos a exames hematológicos e bioquímicos séricos no pré e pós-operató-rio (7 dias). O protocolo anestésico foi: sedação (morfina e acepromazina), indução (propofol), manutenção (isoflurano), analgesia trans-operatória (fentanil Lymphatic mapping has been performed in humans and dogs. Although several cases of anaphylaxis have been reported in humans, there are no such reports in dogs. The objective of this study was to identify the occurrence of adverse reactions to patent blue V dye in bitches undergoing uterine lymphatic mapping procedures using cardiovascular and hematological evaluations. The experiment was performed in 14 mongrel bitches without any reproductive disease, randomly assigned into two equal groups (PBV-uterine lynphatic mapping and OHE; Control -OHE only). The animals were submitted to pre-and postoperative hematological and serum biochemistry exams (7 days). The anesthetic protocol was: sedation (morphine and acepromazine), induction (propofol), maintenance (isoflurane), transoperative analgesia (fentanyl). Systolic blood pressure was monitored throughout the procedure and arterial blood gas analysis was performed immediate pre and postoperatively. For lymphatic mapping was injected patent blue V in the uterine wall, 10 minutes before OEH. Comparisons between the pre-and postoperative parameters within the same animal were performed using the Wilcoxon-Mann-Whitney test. To compare the values between control and PBV group was obtaining the difference between the pre and post of each group, subjected to the Mann-Whitney test (significance of 5%). Differences were observed (P<0.05) between the pre-and postoperative evaluations in the PBV (total protein and the albumin serum), in both groups (arterial partial pressure of oxygen) and in the Control (arterial oxygen saturation). There were no signs of adverse reactions to the patent blue V dye in the healthy bitches submitted to lymphatic uterine mapping.INDEX TERMS: Uterine lymphatic mapping, patent blue V dye, anaphylaxis, hypersensitivity, bitches, vital dye.

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Experimental canine model for sentinel lymph node biopsy in the vulva using technetium and patent blue dye

Cited by 7 publications

References 17 publications

Metastasis of Mammary Carcinoma in Bitches: Evaluation of the Sentinel Lymph Node Technique

Metastasis of Mammary Carcinoma in Bitches: Evaluation of the Sentinel Lymph Node Technique

Comparison of surgical resection of Axillary Lymph Nodes in Dogs with Mammary Gland Tumors with or without sentinel lymph node visualization with patent blue dye

Evaluation of physiological parameters of dogs submitted to uterine lymphatic mapping using patent blue V dye for anaphylaxis detection

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