Exosomes Derived From Human Gingival Mesenchymal Stem Cells Attenuate the Inflammatory Response in Periodontal Ligament Stem Cells

Sun, Jiayao; Wang, Zhiguo; Liu, Peng; Hu, Yingzhe; Li, Tingting; Yang, Jian‐Bo; Gao, Pengyu; Xu, Quanchen

doi:10.3389/fchem.2022.863364

Cited by 20 publications

(14 citation statements)

References 63 publications

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“…These results are consistent with our observations. Our previous studies have indicated that GMSC‐Exo can down‐regulate Wnt5a expression in the inflammatory microenvironment (Sun et al, 2022). Wnt5a is a vital factor in the non‐classical Wnt pathway and is also highly expressed in periodontal inflammatory microenvironments (Sun et al, 2022).…”

Section: Discussionmentioning

confidence: 99%

“…Our previous studies have indicated that GMSC‐Exo can down‐regulate Wnt5a expression in the inflammatory microenvironment (Sun et al, 2022). Wnt5a is a vital factor in the non‐classical Wnt pathway and is also highly expressed in periodontal inflammatory microenvironments (Sun et al, 2022). It has been shown that the non‐classical Wnt pathway engages in crosstalk with the classical Wnt signalling pathway in the inflammatory microenvironment and jointly regulates the osteogenic differentiation of PDLSCs (Liu et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies have found that exosomes originating from gingival mesenchymal stem cells (GMSC‐Exo) can regulate the inflammatory microenvironment (Wang, Ji, et al, 2020; Zhang et al, 2021) by exerting its anti‐inflammatory effects on the NF‐κB pathway (Sun et al, 2022). Moreover, a number of studies have demonstrated that Wnt/β‐catenin signalling activation is a key modulator of gingival‐derived stem cell‐derived exosomes in promoting the proliferation and osteogenic differentiation of PDLSCs (Mai et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

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Human gingival mesenchymal stem cell‐derived exosomes cross‐regulate the Wnt/β‐catenin and NF‐κB signalling pathways in the periodontal inflammation microenvironment

Wang

Fan

et al. 2023

J Clinic Periodontology

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Aim: To examine the immunomodulatory effect of exosomes originating from gingival mesenchymal stem cells (GMSC-Exo) on periodontal bone regeneration and its role in the regulation of the nuclear-factor kappaB (NF-κB) and Wnt/β-catenin pathways in the periodontal inflammatory microenvironment. Materials and Methods: First, periodontal ligament stem cells (PDLSCs) were treated with GMSC-Exo or Porphyromonas gingivalis-derived lipopolysaccharide (P.g-LPS) in vitro. Quantitative real-time PCR (qRT-PCR) and western blot were carried out to detect the expressions of osteogenic differentiation-related factors in cells. Further, PDLSCs were treated with P.g-LPS or inhibitors. The expression of NF-κB pathwayrelated factors as well as of Wnt/β-catenin pathway-related factors were detected by qRT-PCR and western blot.Results: GMSC-Exo treatment promoted the expression of osteogenic differentiationrelated factors within PDLSCs in both normal and inflammatory environments. Further investigations showed that GMSC-Exo could also inhibit the P.g-LPS-induced activation of the NF-κB pathway, leading to the up-regulation of the Wnt/β-catenin pathway.When the Wnt/β-catenin signalling was blocked, the inhibitory effect of GMSC-Exo on the NF-κB pathway was abolished.Conclusions: GMSC-Exo could promote the osteogenic differentiation of PDLSCs.There could be mutually exclusive regulatory roles between the NF-κB and Wnt/βcatenin signalling pathways in a periodontal inflammatory environment. GMSC-Exo exhibited an effective cross-regulation ability for both pathways.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Human gingival mesenchymal stem cell‐derived exosomes cross‐regulate the Wnt/β‐catenin and NF‐κB signalling pathways in the periodontal inflammation microenvironment

Wang

Fan

et al. 2023

J Clinic Periodontology

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“…Flow cytometric analysis revealed positive expressions for the surface markers CD29, CD73, and CD90 and the negative expression of CD45 (Figure 2E–H). 28 …”

Section: Resultsmentioning

confidence: 99%

Copper‐deposited diatom‐biosilica enhanced osteogenic potential in periodontal ligament stem cells and rat cranium

Feng

Wang

et al. 2023

J Biomed Mater Res

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This study aimed to establish that copper-deposited Diatom-biosilica have the potential and possibility for clinical applications in repairing bone defects in a state of inflammation, such as periodontitis. Treatment of alveolar bone defects caused by periodontitis is a major challenge for clinicians. To achieve better repair results, the material should not only be bone conductive but also have the ability to stimulate osteogenesis and angiogenesis at the lesion site. Copper (II) and silicon (IV) ions could react to form basic copper silicate, which promoted both osteogenesis and angiogenesis. The mineralized diatom (Cu-DBs) loaded with copper (II) ions were synthesized by processing diatom shells using a hydrothermal method. Periodontal ligament stem cells (PDLSCs) are used to detect the osteogenic properties of Cu-DBs at the gene and protein levels. Using a rat cranial defect model and a fullthickness skin incision model to test the osteogenic properties of Cu-DBs in vivo.Compared with untreated diatoms (DBs), Cu-DBs extract significantly promoted the expression of osteogenesis-related factors like ALP, RUNX2, BSP, OCN, and OPN in PDLSCs. In vivo experiments further confirmed that Cu-DBs could effectively stimulate the osteogenesis of a rat skull defect and promote angiogenesis, significantly inhibit the inflammatory responses to bone damages, and reduce the infiltration of inflammatory immune cells to the lesion site. Due to the unique chemical characteristics of Si 4+ and Cu 2+ ions, the Cu-DBs composite biomaterial could enhance the osteogenic differentiation of PDLSCS in vitro, as well as stimulate the osteogenesis of the rat in vivo.

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“…EVs inherit the function of their parent cells and have lower immunogenicity; therefore, they play important roles in immune regulation and tissue regeneration [ 106 ]. For example, EVs derived from human GMSCs (GMSC-EVs) could inhibit the inflammatory response of PDLSCs by regulating the expression of NF-κB signaling and Wnt5a, which restored the regenerative potential of PDLSCs and promoted periodontal tissue regeneration in patients with periodontitis [ 107 ].…”

Section: Evs In Pulp Regenerationmentioning

confidence: 99%

Extracellular Vesicles for Dental Pulp and Periodontal Regeneration

Lai

Kou

et al. 2023

Pharmaceutics

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Extracellular vesicles (EVs) are lipid bound particles derived from their original cells, which play critical roles in intercellular communication through their cargoes, including protein, lipids, and nucleic acids. According to their biogenesis and release pathway, EVs can be divided into three categories: apoptotic vesicles (ApoVs), microvesicles (MVs), and small EVs (sEVs). Recently, the role of EVs in oral disease has received close attention. In this review, the main characteristics of EVs are described, including their classification, biogenesis, biomarkers, and components. Moreover, the therapeutic mechanism of EVs in tissue regeneration is discussed. We further summarize the current status of EVs in pulp/periodontal tissue regeneration and discuss the potential mechanisms. The therapeutic potential of EVs in pulp and periodontal regeneration might involve the promotion of tissue regeneration and immunomodulatory capabilities. Furthermore, we highlight the current challenges in the translational use of EVs. This review would provide valuable insights into the potential therapeutic strategies of EVs in dental pulp and periodontal regeneration.

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Exosomes Derived From Human Gingival Mesenchymal Stem Cells Attenuate the Inflammatory Response in Periodontal Ligament Stem Cells

Cited by 20 publications

References 63 publications

Human gingival mesenchymal stem cell‐derived exosomes cross‐regulate the Wnt/β‐catenin and NF‐κB signalling pathways in the periodontal inflammation microenvironment

Human gingival mesenchymal stem cell‐derived exosomes cross‐regulate the Wnt/β‐catenin and NF‐κB signalling pathways in the periodontal inflammation microenvironment

Copper‐deposited diatom‐biosilica enhanced osteogenic potential in periodontal ligament stem cells and rat cranium

Extracellular Vesicles for Dental Pulp and Periodontal Regeneration

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