Extracellular Vesicles for Dental Pulp and Periodontal Regeneration

Lai, Hongbin; Li, Jiaqi; Kou, Xiaoxing; Mao, Xueli; Zhao, Wei; Ma, Lan

doi:10.3390/pharmaceutics15010282

Cited by 12 publications

(9 citation statements)

References 145 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…4 A) [ 147 ]. Based on their biogenesis, EVs can be categorized into three main types: apoptotic bodies, microvesicles, and exosomes [ 148 ]. For example, exosomes derived from DPSCs cultured in odontogenic differentiation media were used to investigate the induction of odontogenic differentiation of DPSCs.…”

Section: Strategies For Regenerative Endodonticsmentioning

confidence: 99%

See 1 more Smart Citation

Dental pulp regeneration strategies: A review of status quo and recent advances

Li,

Fan,

Fan

2024

Bioactive Materials

View full text Add to dashboard Cite

Section: Strategies For Regenerative Endodonticsmentioning

confidence: 99%

“…Reprinted with permission from Ref. [ 148 ]. (B) Regeneration of tissue in periodontal defects mediated by transplanted hDPSCs.…”

Section: Strategies For Regenerative Endodonticsmentioning

confidence: 99%

Dental pulp regeneration strategies: A review of status quo and recent advances

Li,

Fan,

Fan

2024

Bioactive Materials

View full text Add to dashboard Cite

“…Compared with exosomes, apoVs can be easier to obtain from tissues. It also contains more bioactive substances suitable for immunomodulation and regeneration ( 189 , 190 ). Although there is currently no study in the treatment of OA with apoVs, considering its biological characteristics, we believe apoVs may be a potential candidate for OA therapy.…”

Section: Conclusion and Perspectivementioning

confidence: 99%

Extracellular vesicles in osteoarthritis of peripheral joint and temporomandibular joint

Yang

Li²,

Fu³

et al. 2023

Front. Endocrinol.

Self Cite

View full text Add to dashboard Cite

Osteoarthritis (OA) is a disabling disease with significant morbidity worldwide. OA attacks the large synovial joint, including the peripheral joints and temporomandibular joint (TMJ). As a representative of peripheral joint OA, knee OA shares similar symptoms with TMJ OA. However, these two joints also display differences based on their distinct development, anatomy, and physiology. Extracellular vesicles (EVs) are phospholipid bilayer nanoparticles, including exosomes, microvesicles, and apoptotic bodies. EVs contain proteins, lipids, DNA, micro-RNA, and mRNA that regulate tissue homeostasis and cell-to-cell communication, which play an essential role in the progression and treatment of OA. They are likely to partake in mechanical response, extracellular matrix degradation, and inflammatory regulation during OA. More evidence has shown that synovial fluid and synovium-derived EVs may serve as OA biomarkers. More importantly, mesenchymal stem cell-derived EV shows a therapeutic effect on OA. However, the different function of EVs in these two joints is largely unknown based on their distinct biological characteristic. Here, we reviewed the effects of EVs in OA progression and compared the difference between the knee joint and TMJ, and summarized their potential therapeutic role in the treatment of OA.

show abstract

“…These cells possess the ability to undergo self-renewal and differentiate into diverse cell types [ 6 ]. Stem cells isolated from various tissues have different biological characteristics and different osteogenic differentiation (OD) abilities [ 7 ]. Among them, the stem cells derived from bone marrow are the most studied, and the dental pulp stem cells (DPSCs) are also widely studied because of their excellent OD potential and accessibility [ 8 , 9 ].…”

Section: Introductionmentioning

confidence: 99%

Inhibition of ZDHHC16 promoted osteogenic differentiation and reduced ferroptosis of dental pulp stem cells by CREB

Liu,

Yu,

Zhou

et al. 2024

BMC Oral Health

View full text Add to dashboard Cite

Background The repair of bone defects caused by periodontal diseases is a difficult challenge in clinical treatment. Dental pulp stem cells (DPSCs) are widely studied for alveolar bone repair. The current investigation aimed to examine the specific mechanisms underlying the role of Zinc finger DHHC-type palmitoyl transferases 16 (ZDHHC16) in the process of osteogenic differentiation (OD) of DPSCs. Methods The lentiviral vectors ZDHHC16 or si-ZDHHC16 were introduced in the DPSCs and then the cells were induced by an odontogenic medium for 21 days. Subsequently, Quantitate Polymerase Chain Reaction (PCR), immunofluorescent staining, proliferation assay, ethynyl deoxyuridine (EdU) staining, and western blot analysis were used to investigate the specific details of ZDHHC16 contribution in OD of DPSCs. Results Our findings indicate that ZDHHC16 exhibited a suppressive effect on cellular proliferation and oxidative phosphorylation, while concurrently inducing ferroptosis in DPSCs. Moreover, the inhibition of ZDHHC16 promoted cell development and OD and reduced ferroptosis of DPSCs. The expression of p-CREB was suppressed by ZDHHC16, and immunoprecipitation (IP) analysis revealed that ZDHHC16 protein exhibited interconnection with cAMP-response element binding protein (CREB) of DPSCs. The CREB suppression reduced the impacts of ZDHHC16 on OD and ferroptosis of DPSCs. The activation of CREB also reduced the influences of si-ZDHHC16 on OD and ferroptosis of DPSCs. Conclusions These findings provide evidences to support a negative association between ZDHHC16 and OD of DPSCs, which might be mediated by ferroptosis of DPSCs via CREB.

show abstract

Extracellular Vesicles for Dental Pulp and Periodontal Regeneration

Cited by 12 publications

References 145 publications

Dental pulp regeneration strategies: A review of status quo and recent advances

Dental pulp regeneration strategies: A review of status quo and recent advances

Extracellular vesicles in osteoarthritis of peripheral joint and temporomandibular joint

Inhibition of ZDHHC16 promoted osteogenic differentiation and reduced ferroptosis of dental pulp stem cells by CREB

Contact Info

Product

Resources

About