Exosome-Shuttled miR-672-5p from Anti-Inflammatory Microglia Repair Traumatic Spinal Cord Injury by Inhibiting AIM2/ASC/Caspase-1 Signaling Pathway Mediated Neuronal Pyroptosis

Zhou, Zheng; Liu, Cong; Bao, Tianyi; Zhao, Xuan; Xiong, Wu; Luo, Chunyang; Yin, Guoyong; Fan, Jin

doi:10.1089/neu.2021.0464

Cited by 42 publications

(19 citation statements)

References 63 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Zhao et al have concluded miR-532-3p to be involved in atherosclerotic plaque formation and suggested that it can be utilized for therapeutic purposes by regulating its expression [58]. Exosomal mir-672-5p has also been studied for therapeutic applications in spinal cord injury as it targets Caspase-1 signaling pathway [59]. Overall, these two miRNAs depict differential expression in pre-diabetes condition and change in their expression upon high glucose might play a critical role in regulating key molecular pathways of DCM.…”

Section: Discussionmentioning

confidence: 99%

Investigating microRNAs in diabetic cardiomyopathy as tools for early detection and therapeutics

Mathur

Saxena

2022

Mol Cell Biochem

View full text Add to dashboard Cite

To profile microRNAs population of glucose-induced cardiomyoblast cell line and identify the differentially expressed microRNAs and their role under pre-diabetes and diabetes condition in vitro. Rat fetal ventricular cardiomyoblast cell line H9c2 was treated with d-glucose to mimic pre-diabetic, diabetic, and high-glucose conditions. Alteration in cellular, nuclear morphology, and change in ROS generation was analyzed through fluorescent staining. Small RNA sequencing was performed using Illumina NextSeq 550 sequencer and was validated using stem-loop qRT-PCR. A large number (~ 100) differential miRNAs were detected in each treated samples as compared to control; however, a similar expression pattern was observed between pre-diabetes and diabetes conditions with the exception for miR-429, miR-101b-5p, miR-503-3p, miR-384-5p, miR-412-5p, miR-672-5p, and miR-532-3p. Functional annotation of differential expressed target genes revealed their involvement in significantly enriched key pathways associated with diabetic cardiomyopathy. For the first time, we report the differential expression of miRNAs (miR-1249, miR-3596d, miR-3586-3p, miR-7b-3p, miR-191, miR-330-3p, miR-328a, let7i-5p, miR-146-3p, miR-26a-3p) in diabetes-induced cardiac cells. Hyperglycemia threatens the cell homeostasis by dysregulation of miRNAs that begins at a glucose level 10 mM and remains undetected. Analysis of differential expressed miRNAs in prediabetes and diabetes conditions and their role in regulatory mechanisms of diabetic cardiomyopathy holds high potential in the direction of using miRNAs as minimally invasive diagnostic and therapeutic tools.

show abstract

Section: Discussionmentioning

confidence: 99%

Investigating microRNAs in diabetic cardiomyopathy as tools for early detection and therapeutics

Mathur

Saxena

2022

Mol Cell Biochem

View full text Add to dashboard Cite

show abstract

“…miR-146a and 142-3p are involved in the TLR signalling pathway [ 58 , 59 , 60 ]. miR-672 and 135b-5p are related to the pyroptosis pathway [ 36 , 37 , 61 ]. Among those, the PI3K-Akt-mTOR and MAPK signalling pathways were validated and changed the miRNAs-related pathway, which was consistent with our bioinformatics analysis.…”

Section: Discussionmentioning

confidence: 99%

Stage- and Subfield-Associated Hippocampal miRNA Expression Patterns after Pilocarpine-Induced Status Epilepticus

Dheen

Tang

et al. 2022

Biomedicines

View full text Add to dashboard Cite

Objective: To investigate microRNA (miRNA) expression profiles before and after pilocarpine-induced status epilepticus (SE) in the cornu ammonis (CA) and dentated gyrus (DG) areas of the mouse hippocampus, and to predict the downstream proteins and related pathways based on bioinformatic analysis. Methods: An epileptic mouse model was established using a pilocarpine injection. Brain tissues from the CA and DG were collected separately for miRNA analysis. The miRNAs were extracted using a kit, and the expression profiles were generated using the SurePrint G3 Mouse miRNA microarray and validated. The intersecting genes of TargetScan and miRanda were selected to predict the target genes of each miRNA. For gene ontology (GO) studies, the parent-child-intersection (pci) method was used for enrichment analysis, and Benjamini-Hochberg was used for multiple test correction. The Kyoto Encyclopedia of Genes and Genomes (KEGG) was used to detect disease-related pathways among the large list of miRNA-targeted genes. All analyses mentioned above were performed at the time points of control, days 3, 14, and 60 post-SE. Results: Control versus days 3, 14, and 60 post-SE: in the CA area, a total of 131 miRNAs were differentially expressed; 53, 49, and 26 miRNAs were upregulated and 54, 10, and 22 were downregulated, respectively. In the DG area, a total of 171 miRNAs were differentially expressed; furthermore, 36, 32, and 28 miRNAs were upregulated and 78, 58, and 44 were downregulated, respectively. Of these, 92 changed in both the CA and DG, 39 only in the CA, and 79 only in the DG area. The differentially expressed miRNAs target 11–1630 genes. Most of these proteins have multiple functions in epileptogenesis. There were 15 common pathways related to altered miRNAs: nine different pathways in the CA and seven in the DG area. Conclusions: Stage- and subfield-associated hippocampal miRNA expression patterns are closely related to epileptogenesis, although the detailed mechanisms need to be explored in the future.

show abstract

“…miR-672-5p was identified as the most crucial miRNA associated with M2-derived exosomes. M2 exosomes enriched with miR-672-5p could inhibit the AIM2/ASC/caspase-1 signaling pathway by inhibiting AIM2 activity to inhibit neuronal pyroptosis, thus promoting functional behavior recovery in SCI mouse model ( Zhou Z. et al, 2022 ). Ge et al investigated the effects of M1-polarized bone marrow-derived macrophages (M1-BMDM), a pro-inflammatory macrophage phenotype, on vascular endothelial cells in SCI.…”

Section: Sevs and Mirna In Sci Pathology And Therapymentioning

confidence: 99%

The role of small extracellular vesicles and microRNA as their cargo in the spinal cord injury pathophysiology and therapy

Sintakova,

Romanyuk

2024

Front. Neurosci.

View full text Add to dashboard Cite

Spinal cord injury (SCI) is a devastating condition with a complex pathology that affects a significant portion of the population and causes long-term consequences. After primary injury, an inflammatory cascade of secondary injury occurs, followed by neuronal cell death and glial scar formation. Together with the limited regenerative capacity of the central nervous system, these are the main reasons for the poor prognosis after SCI. Despite recent advances, there is still no effective treatment. Promising therapeutic approaches include stem cells transplantation, which has demonstrated neuroprotective and immunomodulatory effects in SCI. This positive effect is thought to be mediated by small extracellular vesicles (sEVs); membrane-bound nanovesicles involved in intercellular communication through transport of functional proteins and RNA molecules. In this review, we summarize the current knowledge about sEVs and microRNA as their cargo as one of the most promising therapeutic approaches for the treatment of SCI. We provide a comprehensive overview of their role in SCI pathophysiology, neuroprotective potential and therapeutic effect.

show abstract

Exosome-Shuttled miR-672-5p from Anti-Inflammatory Microglia Repair Traumatic Spinal Cord Injury by Inhibiting AIM2/ASC/Caspase-1 Signaling Pathway Mediated Neuronal Pyroptosis

Cited by 42 publications

References 63 publications

Investigating microRNAs in diabetic cardiomyopathy as tools for early detection and therapeutics

Investigating microRNAs in diabetic cardiomyopathy as tools for early detection and therapeutics

Stage- and Subfield-Associated Hippocampal miRNA Expression Patterns after Pilocarpine-Induced Status Epilepticus

The role of small extracellular vesicles and microRNA as their cargo in the spinal cord injury pathophysiology and therapy

Contact Info

Product

Resources

About