Exosome-mediated delivery of kartogenin for chondrogenesis of synovial fluid-derived mesenchymal stem cells and cartilage regeneration

Xu, Xiao; Liang, Yujie; Li, Xingfu; Ouyang, Kan; Wang, Manyi; Cao, Tong; Li, Wen‐Cui; Liu, Jianquan; Xiong, Jianyi; Li, Biquan; Xia, Jiang; Wang, Daping; Li, Duan

doi:10.1016/j.biomaterials.2020.120539

Cited by 205 publications

(128 citation statements)

References 67 publications

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“…In a recent report, we engineered chondrocyte affinity peptide (CAP, DWRVIIPPRPSA) exosomes that specifically delivered miRNA-140(miR-140)to chondrocytes in the joints and attenuated the progression of osteoarthritis (OA) in a rat model ( Figure 5 ) 52 . Exosomes displaying the mesenchymal stem cell (MSC) affinity peptide E7 have also been used to deliver a small molecule kartogenin (KGN) to synovial fluid-derived MSCs (SF-MSCs) to promote their chondrogenic differentiation and repair OA cartilage 53 . Despite the effectiveness of this genetic method, the targeting peptide can be degraded in some situations.…”

Section: Surface Engineeringmentioning

confidence: 99%

Engineering exosomes for targeted drug delivery

Liang¹,

Li²,

Lu³

et al. 2021

Theranostics

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647

370

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Exosomes are cell-derived nanovesicles that are involved in the intercellular transportation of materials. Therapeutics, such as small molecules or nucleic acid drugs, can be incorporated into exosomes and then delivered to specific types of cells or tissues to realize targeted drug delivery. Targeted delivery increases the local concentration of therapeutics and minimizes side effects. Here, we present a detailed review of exosomes engineering through genetic and chemical methods for targeted drug delivery. Although still in its infancy, exosome-mediated drug delivery boasts low toxicity, low immunogenicity, and high engineerability, and holds promise for cell-free therapies for a wide range of diseases.

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Section: Surface Engineeringmentioning

confidence: 99%

Engineering exosomes for targeted drug delivery

Liang¹,

Li²,

Lu³

et al. 2021

Theranostics

Self Cite

647

370

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show abstract

“…According to publication numbers, exosomes have attracted more attention among researchers. Currently, exosomes have been identi ed from multiple MSCs, including those derived from AD-MSCs, BM-MSCs, US-MSCs, hESCs, and iPSCs 36,37 .When MSCs sense changes in the surrounding microenvironment, they can secrete exosomes to interact with other cells. Exosomes have been proven to regulate the biological behaviour of chondrocytes, to modulate immune responses and to restore homeostasis to the ECM, thereby promoting cartilage regeneration 38,39 .…”

Section: Discussionmentioning

confidence: 99%

Mapping Thematic Trends and Analysing Hotspots Concerning the Use of Stem Cells for Cartilage Regeneration: a Bibliometric Analysis From 2010 to 2020

Xia¹,

et al. 2021

Preprint

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Background: Defects of articular cartilage are a common condition that usually progresses to osteoarthritis with pain and dysfunction of the joint. Current treatment strategies have had limited success in these patients. Stem cells are emerging as a promising option for cartilage regeneration. We aim to summarize the developmental history of stem cells for cartilage regeneration and to analyse the trends and hotspots.Methods: We screened all relevant literature on stem cells for cartilage regeneration from Web of Science during 2010-2020 and analysed the research trends in this field by VOSviewer and CiteSpace. We also summarized previous clinical trials.Results: We screened 1,011 publications. China contributed the largest number of publications (317, 31.36%) and citations (81,376, 48.61%). The United States has the highest H-index (39). Shanghai Jiao Tong University had the largest number of publications (34) among all full-time institutions. The Journal of Biomaterials and Stem Cell Research & Therapy published the largest number of studies on stem cells for cartilage regeneration (35). SEKIYA I and YANG F published the majority of articles in this field (14), while TOH WS was cited most frequently (740). Regarding clinical research on stem cells for cartilage regeneration, the keyword “double-blind” emerged in recent years, with an average year of 2018.75. In tissue engineering, the keyword “3D printing” appeared latest, with an average year of 2019.625. In biological studies, the key word “extracellular vesicles” appeared latest, with an average year of 2018.9091. The current research trend indicates that basic research is gradually transforming into tissue engineering. Clinical trials have confirmed the safety and feasibility of stem cells for cartilage regeneration.Conclusions: Multiple scientific methods were employed to reveal productivity, collaborations, and research hotspots related to the use of stem cells for cartilage regeneration. 3D printing, extracellular vesicles, and double-blind clinical trials are research hotspots and are likely to be promising in the near future. Further studies are needed for to improve our understanding of this field, and clinical trials with larger sample sizes and longer follow-up periods are needed for clinical transformation.

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“…MSCs binding peptide E7 is fused with exosome membrane protein Lamp 2b to produce exosomes with surface display E7 peptide (E7 exo). The E7 exo containing KGN could effectively enter MSCs, and the degree of cartilage differentiation is higher than that of KGN alone or without E7 exo (Xu et al, 2021). The E7 exo containing KGN pretreated bone marrow MSCs have higher cartilage repair efficiency, stronger cartilage matrix formation and less degradation than those from bone marrow MSCs (Liu et al, 2020).…”

Section: Exosomes Derived From Synovial Mesenchymal Stem Cellsmentioning

confidence: 95%

The Research Progress of Exosomes in Osteoarthritis, With Particular Emphasis on the Mediating Roles of miRNAs and lncRNAs

et al. 2021

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Osteoarthritis (OA) is a kind of degenerative disease, which is caused by many factors such as aging, obesity, strain, trauma, congenital joint abnormalities, joint deformities. Exosomes are mainly derived from the invagination of intracellular lysosomes, which are released into the extracellular matrix after fusion of the outer membrane of multi vesicles with the cell membrane. Exosomes mediate intercellular communication and regulate the biological activity of receptor cells by carrying non-coding RNA, long noncoding RNAs (lncRNAs), microRNAs (miRNAs), proteins and lipids. Evidences show that exosomes are involved in the pathogenesis of OA. In view of the important roles of exosomes in OA, this paper systematically reviewed the roles of exosomes in the pathogenesis of OA, including the roles of exosomes in OA diagnosis, the regulatory mechanisms of exosomes in the pathogenesis, and the intervention roles of exosomes in the treatment of OA. Reviewing the roles of exosomes in OA will help to clarify the pathogenesis of OA and explore new diagnostic biomarkers and therapeutic targets.

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Exosome-mediated delivery of kartogenin for chondrogenesis of synovial fluid-derived mesenchymal stem cells and cartilage regeneration

Cited by 205 publications

References 67 publications

Engineering exosomes for targeted drug delivery

Engineering exosomes for targeted drug delivery

Mapping Thematic Trends and Analysing Hotspots Concerning the Use of Stem Cells for Cartilage Regeneration: a Bibliometric Analysis From 2010 to 2020

The Research Progress of Exosomes in Osteoarthritis, With Particular Emphasis on the Mediating Roles of miRNAs and lncRNAs

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