Engineering exosomes for targeted drug delivery

Liang, Yujie; Li, Duan; Lu, Jianping; Xia, Jiang

doi:10.7150/thno.52570

Cited by 668 publications

(521 citation statements)

References 99 publications

(46 reference statements)

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“…They can carry various biomolecules, including plasmids, proteins, siRNAs, and miRNAs, that could serve as cell-free therapies ( Duan et al, 2020b ; Liang et al, 2021b ). Thus, engineered exosomes have been used for targeted drug delivery ( Duan et al, 2021 ; Liang et al, 2021a ). Our studies have already proven the potential of targeted exosomes for miRNA and small molecular delivery in cartilage repair ( Duan et al, 2020a ; Liang et al, 2020 ; Xu et al, 2021 ).…”

Section: Crispr/cas9 Nano-delivery Approachesmentioning

confidence: 99%

Nanoparticle Delivery of CRISPR/Cas9 for Genome Editing

et al. 2021

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The emerging clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated system (Cas) gene-editing system represents a promising tool for genome manipulation. However, its low intracellular delivery efficiency severely compromises its use and potency for clinical applications. Nanocarriers, such as liposomes, polymers, and inorganic nanoparticles, have shown great potential for gene delivery. The remarkable development of nanoparticles as non-viral carriers for the delivery of the CRISPR/Cas9 system has shown great promise for therapeutic applications. In this review, we briefly summarize the delivery components of the CRISPR/Cas9 system and report on the progress of nano-system development for CRISPR/Cas9 delivery. We also compare the advantages of various nano-delivery systems and their applications to deliver CRISPR/Cas9 for disease treatment. Nano-delivery systems can be modified to fulfill the tasks of targeting cells or tissues. We primarily emphasize the novel exosome-based CRISPR/Cas9 delivery system. Overall, we review the challenges, development trends, and application prospects of nanoparticle-based technology for CRISPR/Cas9 delivery.

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Section: Crispr/cas9 Nano-delivery Approachesmentioning

confidence: 99%

Nanoparticle Delivery of CRISPR/Cas9 for Genome Editing

et al. 2021

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“…The exosomal content is not random and is controlled by posttranslational modifications and specific endogenous target sequences at the stage of exosome formation, most importantly, miRNAs are the most preferred exosome loaded RNAs [116]. Nevertheless, due to the improvement in the exosome efficiency in therapy, their function can also be boosted by the engineering content modifications through genetic and chemical methods and may be used as an ideal drug delivery agent [109,117].…”

Section: Control Of the Secretion Of Extracellular Vesiclesmentioning

confidence: 99%

Adipose-Derived Stem Cells Secretome and Its Potential Application in “Stem Cell-Free Therapy”

Trzyna

Banaś-Ząbczyk

2021

Biomolecules

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Adipose-derived stem cells (ASCs) secrete many cytokines, proteins, growth factors, and extracellular vesicles with beneficial outcomes that can be used in regenerative medicine. It has great potential, and the development of new treatment strategies using the ASCs secretome is of global interest. Besides cytokines, proteins, and growth factors, the therapeutic effect of secretome is hidden in non-coding RNAs such as miR-21, miR-24, and miR-26 carried via exosomes secreted by adequate cells. The whole secretome, including ASC-derived exosomes (ASC-exos) has been proven in many studies to have immunomodulatory, proangiogenic, neurotrophic, and epithelization activity and can potentially be used for neurodegenerative, cardiovascular, respiratory, inflammatory, and autoimmune diseases as well as wound healing treatment. Due to limitations in the use of stem cells in cell-based therapy, its secretome with emphasis on exosomes seems to be a reasonable and safer alternative with increased effectiveness and fewer side effects. Moreover, the great advantage of cell-free therapy is the possibility of biobanking the ASCs secretome. In this review, we focus on the current state of knowledge on the use of the ASCs secretome in stem cell-free therapy.

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“…Although our understanding of exosome biogenesis, release, uptake, and function are not completely clear [23][24][25][26], exosome-focused research remains topical and extensive, which can be ascribed to the potential capability of exosomes in the diagnosis and treatment of various diseases, including neurodegeneration [27], metabolic disorders [28], cardiovascular dysfunction [29], and cancer [30]. Furthermore, exosomes have been widely used in targeted drug delivery through surface engineering techniques, which provides effective strategies for diagnosis and treatment, especially in cancer [31].…”

Section: The Biogenesis Of Exosomesmentioning

confidence: 99%

Exosomes: Advances, development and potential therapeutic strategies in diabetic nephropathy

Chen¹,

Zhang²,

Li³

et al. 2021

Metabolism

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Exosomes, a major type of extracellular vesicles (EVs), are nanoscale vesicles excreted by almost all cell types via invagination of the endosomal membrane pathway. Exosomes play a crucial role in the mediation of intercellular communication both in health and disease, which can be ascribed to their capacity to be transported to neighboring or distant cells, thus regulating the biological function of recipient cells through cargos such as DNA, mRNA, proteins and microRNA. Diabetic nephropathy (DN) is a serious microvascular complication associated with diabetes mellitus as well as a significant cause of end-stage renal disease worldwide, which has resulted in a substantial economic burden on individuals and society. However, despite extensive efforts, therapeutic approaches that prevent the progression of DN do not exist, which implies new approaches are required. An increasing number of studies suggest that exosomes are involved in the pathophysiological processes associated with DN, which may potentially provide novel biomarkers and therapeutic targets for DN. Hence, this review summarizes recent advances involving exosome mechanisms in DN and their potential as biomarkers and therapeutic targets.

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Engineering exosomes for targeted drug delivery

Cited by 668 publications

References 99 publications

Nanoparticle Delivery of CRISPR/Cas9 for Genome Editing

Nanoparticle Delivery of CRISPR/Cas9 for Genome Editing

Adipose-Derived Stem Cells Secretome and Its Potential Application in “Stem Cell-Free Therapy”

Exosomes: Advances, development and potential therapeutic strategies in diabetic nephropathy

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