Exosome-like Nanoparticles: A New Type of Nanocarrier

Fernandes, Mário Marques; Lopes, Ivo; Teixeira, J. A.; Botelho, C. M.; Gomes, Andreia C.

doi:10.2174/0929867326666190129142604

Cited by 29 publications

(21 citation statements)

References 155 publications

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“…In the field of regenerative medicine, the world's most trending fields are exosomes and EVs [48,49]. Treatment using cells is difficult to obtain approval by FDA regulations, and biomaterials such as the ECM, alginate [50], and hydroxyapatite [51] are used for tissue repair and replacement, but their effectiveness as a treatment is insufficient.…”

Section: Discussionmentioning

confidence: 99%

Characterization of Marine Organism Extracellular Matrix-Anchored Extracellular Vesicles and Their Biological Effect on the Alleviation of Pro-Inflammatory Cytokines

Kim

et al. 2021

Marine Drugs

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Representative marine materials such as biopolymers and bioceramics contain bioactive properties and are applied in regenerative medicine and tissue engineering. The marine organism-derived extracellular matrix (ECM), which consists of structural and functional molecules, has been studied as a biomaterial. It has been used to reconstruct tissues and improve biological functions. However, research on marine-derived extracellular vesicles (EVs) among marine functional materials is limited. Recent studies on marine-derived EVs were limited to eco-system studies using bacteria-released EVs. We aimed to expand the range of representative marine organisms such as fish, crustaceans, and echinoderms; establish the extraction process; and study the bioactivity capability of marine EVs. Results confirmed that marine organism ECM-anchored EVs (mEVs) have a similar morphology and cargos to those of EVs in land animals. To investigate physiological effects, lipopolysaccharide (LPS)-infected macrophages were treated with EVs derived from sea cucumber, fish, and shrimp. A comparison of the expression levels of inflammatory cytokine genes revealed that all types of mEVs alleviated pro-inflammatory cytokines, although to different degrees. Among them, the sea cucumber-derived EVs showed the strongest suppression ability. This study showed that research on EVs derived from various types of marine animals can lead to the development of high value-added therapeutics from discarded marine wastes.

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Section: Discussionmentioning

confidence: 99%

Characterization of Marine Organism Extracellular Matrix-Anchored Extracellular Vesicles and Their Biological Effect on the Alleviation of Pro-Inflammatory Cytokines

Kim

et al. 2021

Marine Drugs

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“…Exosome-like nanoparticles have also been revised as a new type of nanocarrier. [38] Because of the broad definition of exosome-like nanoparticles, we chose not to elaborate further in this review.…”

Section: Exosome Mimetics or Exosome Like Nanoparticlesmentioning

confidence: 99%

Exosomes as Targeted Delivery Platform of CRISPR/Cas9 for Therapeutic Genome Editing

Ouyang

Wang

et al. 2021

ChemBioChem

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Therapeutic genome editing harnesses the power of genome editing tools to correct erroneous genes associated with disease pathology. To bring the CRISPR/Cas9 tool from the bench to the bedside, a critical hurdle is the safe and efficient delivery of this nucleic acid tool to the desired type of cells in patients. This review discusses the use of natural carriers, extracellular vesicles (EVs), in particular exosomes, to fill the gap. Exosomes are lipidcontaining nanovesicle released by various types of cells to mediate cell-cell communications. Their inherent long-distance transportation capability, biocompatibility, and engineerability have made EVs potential vehicles for delivering therapeutic drugs. We summarize the recent progress of harnessing exosomes as delivery vehicles for the CRISPR/Cas system to achieve therapeutic gene editing for disease treatment, with a focus on various strategies to achieve selective delivery to a particular type of cell and efficient packaging of the genome editing tools in the vesicles. Critical issues and possible solutions in the design and engineering of the targeting vehicles are highlighted. Taken together, we demonstrate EV/ exosome-mediated packaging of the nucleic acid/protein tools and the cell/tissue-targeted delivery to be a viable way towards the clinical translation of the CRISPR/Cas9 technology.

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“…The collection of exosome-like nanoparticles from high-efficiency plants can be a good alternative to other methods, since they are easier to extract and do not have the drawback of being toxic in animal cells. Natural and synthetic exosome-like nanoparticles, produced from serial extrusion of cells or by bottom-up synthesis, are currently some of the most promising, high-efficiency, biocompatible systems for drug delivery [ 82 ].…”

Section: Nanoparticle-based Carriers In Crispr/casmentioning

confidence: 99%

Exosome/Liposome-like Nanoparticles: New Carriers for CRISPR Genome Editing in Plants

Alghuthaymi

Ahmad

Khan

et al. 2021

IJMS

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Rapid developments in the field of plant genome editing using clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) systems necessitate more detailed consideration of the delivery of the CRISPR system into plants. Successful and safe editing of plant genomes is partly based on efficient delivery of the CRISPR system. Along with the use of plasmids and viral vectors as cargo material for genome editing, non-viral vectors have also been considered for delivery purposes. These non-viral vectors can be made of a variety of materials, including inorganic nanoparticles, carbon nanotubes, liposomes, and protein- and peptide-based nanoparticles, as well as nanoscale polymeric materials. They have a decreased immune response, an advantage over viral vectors, and offer additional flexibility in their design, allowing them to be functionalized and targeted to specific sites in a biological system with low cytotoxicity. This review is dedicated to describing the delivery methods of CRISPR system into plants with emphasis on the use of non-viral vectors.

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Exosome-like Nanoparticles: A New Type of Nanocarrier

Cited by 29 publications

References 155 publications

Characterization of Marine Organism Extracellular Matrix-Anchored Extracellular Vesicles and Their Biological Effect on the Alleviation of Pro-Inflammatory Cytokines

Characterization of Marine Organism Extracellular Matrix-Anchored Extracellular Vesicles and Their Biological Effect on the Alleviation of Pro-Inflammatory Cytokines

Exosomes as Targeted Delivery Platform of CRISPR/Cas9 for Therapeutic Genome Editing

Exosome/Liposome-like Nanoparticles: New Carriers for CRISPR Genome Editing in Plants

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