Exoproduction and Biochemical Characterization of a Novel Thermophilic Serine Protease From Ornithinibacillus Caprae L9T With Hide-dehairing Activity

Li, Xiaoguang; Zhang, Qian; Gan, Longzhan; Jiang, Guo‐Fang; Tian, Yongqiang; Shi, Bi

doi:10.21203/rs.3.rs-573853/v1

Cited by 2 publications

(2 citation statements)

References 31 publications

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“…Recently, several proteolytic bacteria of the genus Bacillus have been tested for waste treatment in the leather industry, due to their properties for the synthesis of hydrolytic enzymes, especially collagenases and keratinases, as well as resistance to environmental factors (Li et al, 2021;Ferdes et al, 2020) The objectives of this study were to isolate and characterise the bacteria producing protease enzyme from solid waste of tannery.…”

Section: Introductionmentioning

confidence: 99%

Isolation and Characterization of Bacterial Protease Enzyme of Leather Waste

Constantinescu¹,

Ferdeş²,

Ignat³

et al. 2022

Proceedings of the 9th International Conference on Advanced Materials and Systems

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The objectives of this study were to isolate and characterize bacteria which produced protease enzyme from tannery solid waste. A solid leather waste sample was used for bacterial isolation, taken from different waste warehouses (solid waste in unhairing phase). Several bacterial strains were isolated from the cultures in Petri dishes, after the growth of the colonies. These strains were characterized in terms of the production of proteolytic enzymes, by a method of screening on the media with casein, which allows the determination of proteolytic indices of microorganisms, the colony diameter, diameter of clear zone, proteolytic index, and enzymatic activities characterization on difference of pH and temperature.

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Section: Introductionmentioning

confidence: 99%

Isolation and Characterization of Bacterial Protease Enzyme of Leather Waste

Constantinescu¹,

Ferdeş²,

Ignat³

et al. 2022

Proceedings of the 9th International Conference on Advanced Materials and Systems

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“…Also, the unhairing time in this study was 24 hours which is almost one-third of the conventional lime-sul de-based unhairing. In previous studies, though lower unhairing time was achieved, complete leather-making was not performed, lacking information about whether the dehaired pelts could be made into usable leathers(Li et al 2021;Mortuza et al 2017;Zekeya et al 2019).…”

mentioning

confidence: 99%

Pre-tanning of Goatskin by Minimizing Chemical Usage using Crude Protease Enzyme for Crust Leather Preparation

Hasan,

Alam,

Mim

et al. 2024

Preprint

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The traditional use of beam house chemicals such as lime and sodium sulfide for hair removal from hide/skin results in significant environmental drawbacks, including excessive water consumption, hair digestion, and solid sludge discharge into effluent. In this study, we have isolated proteolytic bacteria belonging to Alcaligenaceae sp. (GenBank accession number OP456525) from the soil of a tannery solid waste dumping yard. These bacteria exhibited robust protease production with optimal activity at 50°C and pH 7.0. By utilizing the crude protease, we achieved complete unhairing of goatskin and enhanced fiber opening, effectively eliminating three highly polluting stages (liming, deliming, and bating) from conventional leather processing. Protease-based unhairing demonstrated significant reductions in pollution load, with decreases of 60.1%, 98.54%, and 61.63% observed in dissolved solids (TDS), suspended solids (TSS), and total solids (TS), respectively, compared to conventional liming methods. The crust leathers thus produced via protease-based unhairing exhibited approximately 25% increased chromium uptake. Furthermore, enzyme treatment led to improved hydrothermal stability and enhanced physico-mechanical properties, including tensile strength, tear strength, and grain crack strength. FESEM images confirmed that the fiber structure of the samples treated with the protease is more compact than conventional leather. Employing a clean, enzyme-based unhairing procedure utilizing Alcaligenaceae sp. crude protease enables the production of eco-leather without the use of sodium sulfide, lime, ammonium-based deliming agents, or bating agents, thereby enhancing occupational health and safety while promoting a more environmentally friendly and sustainable approach to leather production.

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Exoproduction and Biochemical Characterization of a Novel Thermophilic Serine Protease From Ornithinibacillus Caprae L9T With Hide-dehairing Activity

Cited by 2 publications

References 31 publications

Isolation and Characterization of Bacterial Protease Enzyme of Leather Waste

Isolation and Characterization of Bacterial Protease Enzyme of Leather Waste

Pre-tanning of Goatskin by Minimizing Chemical Usage using Crude Protease Enzyme for Crust Leather Preparation

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