Exocytosis: The Common Release Mechanism of Secretory Granules in Glandular Cells, Neurosecretory Cells, Neurons and Paraneurons

Nagasawa, Junichiro

doi:10.1679/aohc1950.40.supplement_31

Cited by 53 publications

(13 citation statements)

References 55 publications

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“…The other proposes the fusion of the plasma membrane between the melanophore and the recipient cell (CHARLES and INGRAM, 1959;IMAKI and CHAVIN, 1975). Melanosomes are generally surrounded by a membrane (JANDE, 1966), and such membrane-bound granules have been thought to be released into the extracellular matrix by exocytosis (NAGASAWA, 1977). Melanosomes detached from the melanophore should have no membrane.…”

Section: Discussionmentioning

confidence: 99%

The occurrence of melanosomes in the newt glomus cell.

Kusakabe

1991

Archives of Histology and Cytology

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Section: Discussionmentioning

confidence: 99%

The occurrence of melanosomes in the newt glomus cell.

Kusakabe

1991

Archives of Histology and Cytology

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“…Morphological details of exocytosis from noradrenergic nerve terminals are lacking (Nagasawa, 1977) but a few micrographs of vesicles fused with terminal membranes have been published. These include SDV and LDV in terminals of rat vas deferens (Fillenz, 1971) and LDV in terminals of human blood vessels (Thureson- Klein et al, 1976a and1976b).…”

Section: Evidence For Exocytosismentioning

confidence: 99%

Catecholamine‐rich cells and varicosities in bovine splenic nerve, vesicle contents and evidence for exocytosis

1979

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The bovine splenic nerve trunk contains mast cells, ganglion cells, small intensely fluorescent (SIF) cells, and varicosities which exhibit a brilliant fluorescence characteristic for noradrenaline (NA) and dopamine (DA) after formaldehyde exposure. All these catecholamine-rich structures could contribute particles to isolated nerve vesicle fractions. Mast cells are recognized ultrastructurally by their large (300-800 nm) dense granules. SIF cells may be represented by cells and processes containing dense cored vesicles (120-140 nm) which are larger than the typical vesicles in axons and terminals. Terminal-like areas with typical large dense cored vesicles (LDV, 75 nm) and small dense cored vesicles (SDV, 45-55 nm) probably correspond to the fluorescent varicosities. The LDV constitute about 40% of all vesicles in terminal-like areas and terminals. Their staining properties indicate the presence of protein, phospholipids, and ATP. Tyramine depletes NA without loss of matrix density. The LDV can fuse with the terminal membrane, and released material outside omega profiles is interpreted to depict exocytosis. Large and small vesicles are easily distinguished from the very large mast cell granules and the moderately dense Schwann cell vesicles. Neither appear to contaminate the LDV fractions but the latter may contain a small population of SIF cell vesicles. Golgi vesicles from the Schwann cells mainly occur in the lighter zones of the gradient.

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“…1967;Benedeczky and Somogyi. 1975;Nagasawa, 1977;Kachi et al, 1985]. We first observed the clear evidence of exocytosis in the PC 12 cells after 1-min exposure to a high concentration of potassium medium.…”

Section: Discussionmentioning

confidence: 90%

“…It has been reported that a high concentration of potas sium in the extracellular medium, which presumably depolarizes the cell membrane and enhances calcium uptake [Rubin, 1970], evoked the calcium-dependent cxocytotic secretion in adrenal medullary cells [Nagasawa, 1977;Schneider et al, 1977;Ishikawa and Kanno. 1978].…”

Section: Discussionmentioning

confidence: 99%

Exocytosis in Pheochromocytoma Cells after Exposure to a High Concentration of Potassium

Oomori¹,

Okuno

Fujisawa

et al. 1991

Cells Tissues Organs

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An electron-microscopic study revealed exocytosis of electron-dense granules in rat pheochromocytoma (PC12) cells after 1-min exposure to a high concentration of potassium medium. Numerous omega profiles indicative of the exocytosis were seen in the surface membrane of the PC12 cells. Most omega profiles showed clear or did not contain the granule contents. Sometimes, the granular, flocculent or dense materials were seen within the omega profiles that were exposed directly to the extracellular medium. Moreover, noncoated or coated omega profiles were observed. Coated vesicles were also visible under the surface membrane. The present study suggests that PC12 cells retain the exocytotic release mechanism for catecholamines.

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Exocytosis: The Common Release Mechanism of Secretory Granules in Glandular Cells, Neurosecretory Cells, Neurons and Paraneurons

Cited by 53 publications

References 55 publications

The occurrence of melanosomes in the newt glomus cell.

The occurrence of melanosomes in the newt glomus cell.

Catecholamine‐rich cells and varicosities in bovine splenic nerve, vesicle contents and evidence for exocytosis

Exocytosis in Pheochromocytoma Cells after Exposure to a High Concentration of Potassium

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