Exercise-induced laryngeal obstruction: a common and overlooked cause of exertional breathlessness

Hall, Andrew B.; Thomas, Mike; Sandhu, Guri; Hull, James H.

doi:10.3399/bjgp16x687001

Cited by 29 publications

(26 citation statements)

References 6 publications

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“…For low aspect pore, access resistance becomes several fold larger than the geometric resistance. The expression for the access resistance of a pore was given by Hall in 1975 [38] and is the contribution of electric field lines converging into the pore from bulk electrolyte solution. Hall gave the expression for access resistance as:…”

Section: Methodsmentioning

confidence: 99%

Low aspect ratio micropores for single‐particle and single‐cell analysis

et al. 2015

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This paper describes microparticle and bacterial translocation studies using low aspect ratio solid-state micropores. Micropores, 5 μm in diameter, were fabricated in 200 nm thick free-standing silicon nitride membranes, resulting in pores with an extremely low aspect ratio, nominally 0.04. For microparticle translocation experiments, sulfonated polystyrene microparticles and magnetic microbeads in size range of 1-4 μm were used. Using the microparticle translocation characteristics, we find that particle translocations result in a change only in the pore's geometrical resistance while the access resistance remains constant. Furthermore, we demonstrate the ability of our micropore to probe high-resolution shape information of translocating analytes using concatenated magnetic microspheres. Distinct current drop peaks were observed for each microsphere of the multibead architecture. For bacterial translocation experiments, nonflagellated Escherichia coli (strain HCB 5) and wild type flagellated Salmonella typhimurium (strain SJW1103) were used. Distinct current signatures for the two bacteria were obtained and this difference in translocation behavior was attributed to different surface protein distributions on the bacteria. Our findings may help in developing low aspect ratio pores for high-resolution microparticle characterization and single-cell analysis.

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Section: Methodsmentioning

confidence: 99%

Low aspect ratio micropores for single‐particle and single‐cell analysis

et al. 2015

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“…We have denoted by R acc the resistance of each semi-infinite half sphere, known as the access resistance (the derivation can be found in Ref. [15]), and by R pore the electric resistance of the pore (Fig. 1b).…”

Section: Modeling the Dna Capturementioning

confidence: 99%

DNA capture into the ClyA nanopore: diffusion-limited versus reaction-limited processes

Nomidis¹,

Hooyberghs²,

Maglia³

et al. 2018

J. Phys.: Condens. Matter

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The capture and translocation of biomolecules through nanometer-scale pores are processes with a potentially large number of applications, and hence they have been intensively studied in recent years. The aim of this paper is to review existing models of the capture process by a nanopore, together with some recent experimental data of short single- and double-stranded DNA captured by the Cytolysin A (ClyA) nanopore. ClyA is a transmembrane protein of bacterial origin which has been recently engineered through site-specific mutations, to allow the translocation of double- and single-stranded DNA. A comparison between theoretical estimations and experiments suggests that for both cases the capture is a reaction-limited process. This is corroborated by the observed salt dependence of the capture rate, which we find to be in quantitative agreement with the theoretical predictions.

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“…In addition, the resistance contribution at the out-of-pore part of the sensing zone originating from the gradual convergence of the electric field lines into the orifice, i. e., the access resistance R access , [46] can be expressed by Eq. (2) Scheme 1.…”

Section: Conical Pore/channelmentioning

confidence: 99%

Counting and Sizing of Single Vesicles/Liposomes by Electrochemical Events

Liu

et al. 2018

ChemElectroChem

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The analysis of single entities in a liquid phase by electrochemical events has received much attention in recent years due to innovations and advances in exciting electrochemical methods as well as precise manufacturing techniques. Vesicles, as a special type of entity, play an important role in biological systems. However, the soft character and complex surface chemistry of vesicles present inherent challenges for single‐vesicle analysis. This minireview mainly focuses on the theory and recent progress of single‐vesicle/liposome analysis based on electrochemical events. Three different analytical principles, namely, pore/channel translocation, microelectrode impact and nanopipette collision, are reviewed and expatiated. Not only the capabilities for size determination, vesicle/liposome counting and the electroactive quantification of the contents within vesicles/liposomes but also the specificities of vesicle/liposome or entity‐pore/electrode interactions reflected in translocation or collision events are discussed in detail. Moreover, the advantages and disadvantages of each method of single‐vesicle analysis are discussed in this minireview.

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Exercise-induced laryngeal obstruction: a common and overlooked cause of exertional breathlessness

Cited by 29 publications

References 6 publications

Low aspect ratio micropores for single‐particle and single‐cell analysis

Low aspect ratio micropores for single‐particle and single‐cell analysis

DNA capture into the ClyA nanopore: diffusion-limited versus reaction-limited processes

Counting and Sizing of Single Vesicles/Liposomes by Electrochemical Events

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