Low aspect ratio micropores for single‐particle and single‐cell analysis

Goyal, Gaurav; Mulero, Rafael; Ali, Jamel; Darvish, Armin; Kim, Min Jun

doi:10.1002/elps.201400570

Cited by 24 publications

(21 citation statements)

References 44 publications

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“…HIV‐1 particles dispersed in PBS (10 mM, pH = 7.4) were added to the cis chamber in our experimental setup reported elsewhere . Upon grounding the cis chamber and applying a positive voltage bias across the nanopore, we observed perturbations in the current trace more formally defined as resistive pulses or events (Fig.…”

Section: Resultsmentioning

confidence: 93%

“…The pore conductance was tested with the open current before loading sample in the cis chamber to ensure that the conductance of the pore was the same as before and no trace of contaminants from previous experiments was left in the pore or flow cells. The size of the pore was estimated using a solution‐based conductance model ,

G = σ {([], \frac{4 L}{π D^{2}} + \frac{1}{D})}^{- 1}

where D is nanopore diameter, L is length and σ is the conductivity of the electrolyte solution. The experiments were done with two unique nanopores comparable in size and conductance.…”

Section: Methodsmentioning

confidence: 93%

Section: Nanopore Resistive Pulse Sensingmentioning

confidence: 99%

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Mechanical characterization of HIV‐1 with a solid‐state nanopore sensor

et al. 2018

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Enveloped viruses fuse with cells to transfer their genetic materials and infect the host cell. Fusion requires deformation of both viral and cellular membranes. Since the rigidity of viral membrane is a key factor in their infectivity, studying the rigidity of viral particles is of great significance in understating viral infection. In this paper, a nanopore is used as a single molecule sensor to characterize the deformation of pseudo-type human immunodeficiency virus type 1 at sub-micron scale. Non-infective immature viruses were found to be more rigid than infective mature viruses. In addition, the effects of cholesterol and membrane proteins on the mechanical properties of mature viruses were investigated by chemically modifying the membranes. Furthermore, the deformability of single virus particles was analyzed through a recapturing technique, where the same virus was analyzed twice. The findings demonstrate the ability of nanopore resistive pulse sensing to characterize the deformation of a single virus as opposed to average ensemble measurements.

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Section: Resultsmentioning

confidence: 93%

G = σ {([], \frac{4 L}{π D^{2}} + \frac{1}{D})}^{- 1}

where D is nanopore diameter, L is length and σ is the conductivity of the electrolyte solution. The experiments were done with two unique nanopores comparable in size and conductance.…”

Section: Methodsmentioning

confidence: 93%

Section: Nanopore Resistive Pulse Sensingmentioning

confidence: 99%

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Mechanical characterization of HIV‐1 with a solid‐state nanopore sensor

et al. 2018

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“…Since the geometry of the nanopore was approximately cylindrical, the following conductance equation was used to derive the analytical value of the nanopore conductance ,

G = σ {([], \frac{4 L}{π D^{2}} + \frac{1}{D})}^{- 1},

where D is the pore diameter and L is the length and conductivity of the 0.1M KCl solution ( σ ) were ∼250 nm (Supporting Information Fig. 4a), ∼180 nm (Supporting Information Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Since the geometry of the nanopore was approximately cylindrical, the following conductance equation was used to derive the analytical value of the nanopore conductance [43],…”

Section: Nanopore Conductance and Electrophoretic Phenomenonmentioning

confidence: 99%

Stiffness measurement of nanosized liposomes using solid‐state nanopore sensor with automated recapturing platform

et al. 2019

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This paper describes a method to gauge the stiffness of nanosized liposomes – a nanoscale vesicle – using a custom‐made recapture platform coupled to a solid‐state nanopore sensor. The recapture platform electrically profiles a given liposome vesicle multiple times through automated reversal of the voltage polarity immediately following a translocation instance to re‐translocate the same analyte through the nanopore – provides better statistical insight at the molecular level by analyzing the same particle multiple times compared to conventional nanopore platforms. The capture frequency depends on the applied voltage with lower voltages (i.e., 100 mV) permitting higher recapture instances than at higher voltages (>200 mV) since the probability of particles exiting the nanopore capture radius increases with voltage. The shape deformation was inferred by comparing the normalized relative current blockade (ΔI/I0̂false) at the two voltage polarities to that of a rigid particle, i.e., polystyrene beads. We found that liposomes deform to adopt a prolate shape at higher voltages. This platform can be further applied to investigate the stiffness of other types of soft matters, e.g., virus, exosomes, endosomes, and accelerate the potential studies in pharmaceutics for increasing the drug packing and unpacking mechanism by controlling the stiffness of the drug vesicles.

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Multiple consecutive recapture of rigid nanoparticles using a solid‐state nanopore sensor

et al. 2017

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Solid-state nanopore sensors have been used to measure the size of a nanoparticle by applying a resistive pulse sensing technique. Previously, the size distribution of the population pool could be investigated utilizing data from a single translocation, however, the accuracy of the distribution is limited due to the lack of repeated data. In this study, we characterized polystyrene nanobeads utilizing single particle recapture techniques, which provide a better statistical estimate of the size distribution than that of single sampling techniques. The pulses and translocation times of two different sized nanobeads (80 nm and 125 nm in diameter) were acquired repeatedly as nanobeads were recaptured multiple times using an automated system controlled by custom-built scripts. The drift-diffusion equation was solved to find good estimates for the configuration parameters of the recapture system. The results of the experiment indicated enhancement of measurement precision and accuracy as nanobeads were recaptured multiple times. Reciprocity of the recapture and capacitive effects in solid state nanopores are discussed. Our findings suggest that solid-state nanopores and an automated recapture system can also be applied to soft nanoparticles, such as liposomes, exosomes, or viruses, to analyze their mechanical properties in single-particle resolution.

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Low aspect ratio micropores for single‐particle and single‐cell analysis

Cited by 24 publications

References 44 publications

Mechanical characterization of HIV‐1 with a solid‐state nanopore sensor

Mechanical characterization of HIV‐1 with a solid‐state nanopore sensor

Stiffness measurement of nanosized liposomes using solid‐state nanopore sensor with automated recapturing platform

Multiple consecutive recapture of rigid nanoparticles using a solid‐state nanopore sensor

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