Excision of sclerocorneal limbus in dogs and resulting clinical events: study of an experimental model

Abstract

The consequences of complete removal of the sclerocorneal limbus epithelium were evaluated, as well as the model for such, in which n-heptanol and superficial keratectomy were used. The association of nheptanol and keratectomy allowed a complete excision of stem cells. Moreover, the destruction of these cells led to significant tectonic alterations that could be employed as a feasible model for clinical experimentation of corneal transplantation.Keywords: dog, stem cell, cornea RESUMO As conseqüências da completa remoção do epitélio do limbo esclerocorneano foram avaliadas

Limbal autograft transplantation in a dog with alkali-induced ulceration

“…However, this technique was not yet routinely in veterinary medicine. BRUNELLI et al (2006) observed ocular, predominantly mucopurulent, secretion in the donor eye of 91.67% of dogs 3 days after limbal stem cell autograft transplantation. At 7 days, only 20% of the animals presented discrete mucoid secretion, whereas no ocular secretion was observed in the remaining 80%.…”

“…Various therapies for the repair of corneal damage have been proposed, including the use of blood serum (JORGE et al, 2003), substances rich in linoleic acid (CALADO et al, 2005), and substances that inhibit metalloproteinases such as extracts of Pothomorphe umbellate (BARROS et al, 2007) and experimental surgical procedures (BRUNELLI et al, 2006). Ciência Rural, v.40, n.8, ago, 2010.…”

“…Limbal transplants have been suggested as possible alternatives (DUA & AZUARA-BLANCO, 1999;BRUNELLI et al, 2006).…”

“…For this purpose, lamellar dissection was performed at the corneal periphery, extending up to 3mm into the conjunctiva, at 11 to 12 and 7 to 9 o'clock. The excised tissue was kept in sterile saline at room temperature immediately after excision (BRUNELLI et al, 2006). The limbal lamellae collected were then transplanted into the affected eye.…”

“…Therefore, limbal transplants have advantages when compared to other therapeutic alternatives. BRUNELLI et al (2006) reported the onset of corneal vascularization by the third day in animals with alkali-induced ulceration Ciência Rural, v.40, n.8, ago, 2010.…”