Excessıve fluorıde ıntake alters the MMP-2, TIMP-1 and TGF-β levels of perıodontal soft tıssues: an experımental study ın rabbıts

Lütfioğlu, Müge; Sakallıoğlu, Elif Eser; Sakallıoğlu, Umur; Gülbahar, M. Y.; Muğlalı, Mehtap; Baş, Burcu; Aksoy, Abdurrahman

doi:10.1007/s00784-011-0652-6

Cited by 10 publications

(7 citation statements)

References 40 publications

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“…An in vitro study shown that MMP-2 activity of human saliva significantly inhibited by excessive fluoride (above 50 mg/L)20. However, an increase of MMP-2 expression in gingival connective tissue and periodontal ligament was found in rabbits treated with 40 mg/L of fluoride21. In a word, though the effect of fluoride on MMP-2 remains assumptive, excessive fluoride exposure could alter MMP-2 activity and expression.…”

Section: Discussionmentioning

confidence: 99%

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Matrix Metallopeptidase-2 Gene rs2287074 Polymorphism is Associated with Brick Tea Skeletal Fluorosis in Tibetans and Kazaks, China

Pei

Liu

et al. 2017

Sci Rep

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Brick tea skeletal fluorosis is still a public health issue in the north-western area of China. However its pathogenesis remains unknown. Our previous study reveals that the severity of skeletal fluorosis in Tibetans is more serious than that in Kazaks, although they have similar fluoride exposure, suggesting the onset of brick tea type skeletal fluorosis might be genetically influenced. Here we show that MMP-2 rs2287074 SNP (G/A), but not rs243865, was associated with Brick tea type fluorosis in Tibetans and Kazaks, China. The trend test reveals a decline in probability for skeletal fluorosis with increasing number of A alleles in Tibetans. After controlling potential confounders, AA genotype had about 80 percent lower probability of developing skeletal fluorosis than GG genotype in Tibetans (odds ratio = 0.174, 95% CI: 0.053, 0.575), and approximately 53 percent lower probability in Kazaks (odds ratio = 0.462, 95% CI: 0.214, 0.996). A meta-analysis shows that the AA genotype had approximately 63 percent lower odds (odds ratio = 0.373, 95% CI: 0.202, 0.689) compared with GG genotype within the two ethnicities. A significant correlation was also found between the genotype of MMP2 rs2287074 and skeletal fluorosis severity. Therefore, the A allele of MMP2 rs2287074 could be a protective factor for brick tea skeletal fluorosis.

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Section: Discussionmentioning

confidence: 99%

“…Recent studies have revealed that fluoride can affect the expression of MMP-2 in cell and animal experiment2021. An epidemiological survey reveals that an increase of MMP-2 is positively correlated with fluoride exposure and the severity of dental fluorosis in adults22.…”

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confidence: 99%

Matrix Metallopeptidase-2 Gene rs2287074 Polymorphism is Associated with Brick Tea Skeletal Fluorosis in Tibetans and Kazaks, China

Pei

Liu

et al. 2017

Sci Rep

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“…However, this was the first time this gene was specifically studied for DDE. In periodontal tissues, excessive fluoride intake increases MMP2 expression [52], but genetic variation of MMP2 was not associated with dental fluorosis [27]. Therefore, genetic variation of MMP2 may be an additional factor leading to enamel defects that can be added to others such as previous orthodontic treatment and/or surgical treatment [53][54][55].…”

Section: Plos Onementioning

confidence: 99%

Maxillary incisor enamel defects in individuals born with cleft lip/palate

et al. 2020

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Cleft lip with or without cleft palate (CLP) is considered the most frequent congenital malformations of the head and neck, with cleft individuals exhibiting more chances of presenting abnormalities such as developmental defects of enamel (DDE). Matrix metallopeptidase 2 (MMP2) is a membrane-bound protein with collagen-degrading ability and has important roles in tooth formation and mineralization. The aim of this study was to evaluate the frequency, location, severity and extent of DDE found in the maxillary incisors for groups of individuals born with CLP, as well as understanding their relationship with the cleft side. Besides, this study addresses the hypothesis that DDE can be influenced by variation in the MMP2 genes (rs9923304). Individual samples, clinical history, intraoral photographs and panoramic radiographs were obtained from 233 patients under treatment at the Cleft Lip and Palate Service of the University Hospital Lauro Wanderley at the Federal University of Paraíba. Digital images were examined by the same evaluator using the Classification of Defects According to the Modified DDE Index, and then loaded into the Image Tool software, where two measurements were made: total area of the buccal surface (SA) and the area of the DDE (DA), obtaining the percentage of the surface area affected (%SAD) (ICC = 0.99). Genomic DNA was extracted from saliva samples from 124 participants. Genotyping was carried out using TaqMan chemistry for one marker in MMP2 (rs9923304). Statistical analyses were performed by The Jamovi Project software. The Shapiro-Wilk test was applied, followed by the Student’s t-test and the Mann-Whitney test. Chi-square and Fisher’s exact tests, and odds ratio (OR) with 95% confidence interval (CI) calculations were used to determine Hardy-Weinberg equilibrium and statistically significant differences with an alpha of 0.05. No significant differences in the prevalence and extent of enamel defects were found between male and female individuals born with CLP (p = 0.058256). The frequency of individuals presenting teeth with DDE, in relation to the cleft and non-cleft side, was statistically different (p <0.001; OR = 7.15, CI: 4.674> 7.151> 10.942). However, the averages of %SAD were similar (p = 0.18). The highest means of the %SAD were found in individuals with bilateral cleft lip with or without cleft palate (BCLP) when compared to individuals with unilateral cleft lip with or without cleft palate (UCLP), for the teeth inside (IA) and outside the cleft area (OA) (p <0.001). Regardless of the cleft side, individuals with BCLP were 7.85 times more likely to have more than one third of the tooth surface affected, showing more frequently defects in the three thirds (OA: p <0.001) (IA: p = 0.03), as well as a higher frequency of more than one type of defect (OA: p = 0.000358) (IA: p = 0.008016), whereas in UCLP, defects were isolated and restricted to only one third, more frequently, the incisal third (OA: p = 0.009) (IA: p = 0.001), with greater frequency of milder defects, such as demarcated (p = 0.02) and diffuse (p = 0.008) opacities. A higher frequency of the T allele, less common, was observed in the group of CLP individuals who had all the affected teeth or at least two teeth with %SAD greater than 20% (p = 0.019843). Our results suggest that MMP2 may have a role in the cases that presented DDE and genotyping rs9923304 could serve as the basis for a genomic approach to define risks for individuals born with CLP. Frequency and severity of DDE is strongly related to the CLP phenotype, since the highest values were found for BCLP. However, the extent of the DDE is independent of its relationship with the side of the cleft.

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“…Fluoride ions can cause increase in production of ROS with subsequent oxidative stress development and lipid peroxidation 7,8 . There are evidences that fluoride can alter state of connective tissue in periodontium 9 . Several studies have shown that fluoride intoxication can lead to increase in concentration of L-hydroxyproline 10,11 , which is the marker of collagen breakdown.…”

Section: Introductionmentioning

confidence: 99%

Influence of combined nitrate and fluoride intoxication on connective tissue disorders in rats gastric mucosa

Akimov¹,

MISCHENKO²,

Костенко³

2019

Arch Balk Med Union

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Influence de l'intoxication combinée nitrate-fluorure sur les troubles tissulaires conjonctifs dans la muqueuse gastrique des rats Introduction. Certaines régions d'Europe et d'Ukraine présentent une concentration élevée de fluorures dans les eaux potables. Une agriculture très développée peut entraîner une concentration accrue de nitrates dans les eaux souterraines et les eaux potables. Par conséquent, leur consommation excessive combinée avec de la nourriture et de l'eau n'est pas exclue. Le but de l'étude est de déterminer l'influence de l'intoxication combinée au fluorure de nitrate sur la concentration de différentes fractions de glycosaminoglycanes (GAG) et la concentration de L-hydroxyproline dans la muqueuse gastrique de rats. Matériaux et méthodes. Les intoxications chroniques ont été induites par une injection intragastrique de fluorure de sodium à une dose de 10 mg / kg, de nitrate de sodium à une dose de 500 mg / kg et de leur association pendant 30 jours. La concentration en GAG a été déterminée par la méthode au carbazole. Pour évaluer les concentrations de différentes fractions de GAG, nous avons utilisé une méthode de précipitation différentielle à l'éthanol. Pour l'estimation de la concentration en L-hydroxyproline, nous avons ABSTRACT Introduction. Certain regions in Europe and Ukraine have high concentration of fluorides in drinking waters. Highly developed agriculture can result in increased concentration of nitrates in ground and drinking waters. Therefore, their combined excessive intake with food and water is not excluded. The objective of the study was to determine the influence of combined nitrate-fluoride intoxication on concentration of different fractions of glycosaminoglycans (GAG) and L-hydroxyproline concentration in gastric mucosa of rats. Materials and methods. Chronic intoxications were induced by intragastric injection of sodium fluoride in a dose of 10 mg/kg, sodium nitrate in a dose of 500 mg/kg and their combination during 30 days. Concentration of GAG was determined by carbazole method. To evaluate concentrations of different fractions of GAG we used the method of differential precipitation with ethanol. For L-Hydroxyproline concentration estimation, we used Hydroxyproline Colorimetric Assay Kit (BioVision K555-100). Results. The total concentration of GAG decreases during chronic fluoride and nitrate intoxications, but increases during combined intoxication. Concentration of Heparin/Heparan GAG fraction and Keratan/Dermatan GAG fraction drops during

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Excessıve fluorıde ıntake alters the MMP-2, TIMP-1 and TGF-β levels of perıodontal soft tıssues: an experımental study ın rabbıts

Abstract: Excessive fluoride consumption may alter the periodontal tissue homeostasis which may be detrimental in the maintenance of periodontal health.

Cited by 10 publications

References 40 publications

Matrix Metallopeptidase-2 Gene rs2287074 Polymorphism is Associated with Brick Tea Skeletal Fluorosis in Tibetans and Kazaks, China

Matrix Metallopeptidase-2 Gene rs2287074 Polymorphism is Associated with Brick Tea Skeletal Fluorosis in Tibetans and Kazaks, China

Maxillary incisor enamel defects in individuals born with cleft lip/palate

Influence of combined nitrate and fluoride intoxication on connective tissue disorders in rats gastric mucosa

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