Influence of combined nitrate and fluoride intoxication on connective tissue disorders in rats gastric mucosa

Abstract

Influence de l'intoxication combinée nitrate-fluorure sur les troubles tissulaires conjonctifs dans la muqueuse gastrique des rats Introduction. Certaines régions d'Europe et d'Ukraine présentent une concentration élevée de fluorures dans les eaux potables. Une agriculture très développée peut entraîner une concentration accrue de nitrates dans les eaux souterraines et les eaux potables. Par conséquent, leur consommation excessive combinée avec de la nourriture et de l'eau n'est pas exclue. Le but de l'étude est de déterminer l'influence de l'intoxication combinée au fluorure de nitrate sur la concentration de différentes fractions de glycosaminoglycanes (GAG) et la concentration de L-hydroxyproline dans la muqueuse gastrique de rats. Matériaux et méthodes. Les intoxications chroniques ont été induites par une injection intragastrique de fluorure de sodium à une dose de 10 mg / kg, de nitrate de sodium à une dose de 500 mg / kg et de leur association pendant 30 jours. La concentration en GAG a été déterminée par la méthode au carbazole. Pour évaluer les concentrations de différentes fractions de GAG, nous avons utilisé une méthode de précipitation différentielle à l'éthanol. Pour l'estimation de la concentration en L-hydroxyproline, nous avons ABSTRACT Introduction. Certain regions in Europe and Ukraine have high concentration of fluorides in drinking waters. Highly developed agriculture can result in increased concentration of nitrates in ground and drinking waters. Therefore, their combined excessive intake with food and water is not excluded. The objective of the study was to determine the influence of combined nitrate-fluoride intoxication on concentration of different fractions of glycosaminoglycans (GAG) and L-hydroxyproline concentration in gastric mucosa of rats. Materials and methods. Chronic intoxications were induced by intragastric injection of sodium fluoride in a dose of 10 mg/kg, sodium nitrate in a dose of 500 mg/kg and their combination during 30 days. Concentration of GAG was determined by carbazole method. To evaluate concentrations of different fractions of GAG we used the method of differential precipitation with ethanol. For L-Hydroxyproline concentration estimation, we used Hydroxyproline Colorimetric Assay Kit (BioVision K555-100). Results. The total concentration of GAG decreases during chronic fluoride and nitrate intoxications, but increases during combined intoxication. Concentration of Heparin/Heparan GAG fraction and Keratan/Dermatan GAG fraction drops during

The state of oxidative-antioxidant homeostasis under the influence of subtoxic doses of sodium fluoride

“…Considering that the condition of the hematopoietic system is an integral indicator of the homeostatic function of the body, an indicator of many diseases and intercurrent poisoning, we studied the effect of subtoxic doses of sodium fluoride on the state of white and red blood in animal experiments under conditions of intoxication [1,14].…”

“…The problem of correlation and interaction of oxidative and reduction processes in biological systems is the leading one in the list of substantiation of pathogenetic mechanisms of the formation of structural and metabolic disorders from organs, systems and body functions [1,18,33]. The structure and function of the oxidative and antioxidant systems of the body is an actual topic in recent years [13,19].…”

Influence of bacterial lipopolysaccharide on the degradation of the components of the extracellular matrix of rat biceps femoris muscle during high fructose diet-induced metabolic syndrome

“…The objects of the study were a 10% homogenate of rat biceps femoris muscle and blood samples from right ventricle. In 10% homogenate, the total concentration of GAG and the concentration of heparin-heparan, keratan-dermatan and chondroitin fractions of GAG were determined [13,14]. The method of differential precipitation according to N. Volpi was used to determine the concentration of individual fractions of GAG.…”

Інтенсивність Синтезу Оксиду Азоту У Крові Щурів Під Впливом Фториду Натрію

“…Ступінь патогенного впливу при розвитку стрес-реакції багато в чому визначається станом стрес-лімітуючих систем, зокрема, системи оксиду азоту (NO). Доведено, що NO-залежна регуляція центральних ланок стресреакції забезпечується тим, що гіпофіз отримує від гіпоталамуса широко представлену NO-ергічну іннервацію [4]. Виявлена властивість NO модулювати вивільнення основних стрес-гормонів гіпофізу дозволяє припустити важливу роль NO в регуляції його функціонування та попередженні надмірної активації центральної ланки стрес-системи.…”