Influence of bacterial lipopolysaccharide on the degradation of the components of the extracellular matrix of rat biceps femoris muscle during high fructose diet-induced metabolic syndrome

Abstract

Metabolic syndrome is an epidemic of non-infectious origin, which is associated with the consumption of a large amount of high-calorie food and a sedentary lifestyle. It is not excluded that bacterial lipopolysaccharides (LPS) may enter the body during metabolic syndrome. The purpose of this work is to study the combined effect of bacterial lipopolysaccharide and high fructose diet on the concentration of glycosaminoglycans and their individual fractions, the content of free L-oxyproline and sialic acids in rat biceps femoris muscle. Bacterial LPS was injected intraperitoneally at a dose of 0.4 μg/kg. Metabolic syndrome was modelled by using 20% fructose solution as only source of drinking water. In the test group of animals total concentration of glycosaminoglycans increased by 22.53%, the heparin-heparan and keratan-dermatan fraction concentrations raised by 26.18 and 35.91%, but concentration of the chondroitin fraction did not change compared to the control. The concentrations of free L-oxyproline and sialic acids increased by 120.71 and 156.78%, respectively. Thus, high fructose diet, stimulation of the organism with bacterial lipopolysaccharide and their combination lead to increased degradation of glycoproteins and proteoglycans and intensify collagenolysis in the biceps femoris muscle of rats.

Influence of organism stimulation with bacterial lipopolysaccharide on the metabolism of the extracellular matrix of the heart of rats under conditions of experimental metabolic syndrome

“…In our previous studies, it was shown that the use of a 20% fructose solution as the only source of drinking water against the background of a standard food diet led to the development of changes in the body of rats that are characteristic of the metabolic syndrome, namely: the development of hyperglycemia, hyperlipidemia, dyslipidemia (with a predominance low-density lipoproteins), hypercholesterolemia, and insulin resistance [8].…”

“…The third group was the group of organism stimulation with bacterial LPS (LPS group). The animals of this group fed on a standard diet and for 60 days received an injection of bacterial LPS S. typhi (Pyrogenal, Medgamal) according to following scheme: on the first week 0.4 μg/kg of bacterial LPS was introduced by intraperitoneal injection three times a week, then once a week for the rest of the experiment (60 days) [8]. The fourth group was the group of the combined На умовах ліцензії CC BY 4.0 effect of organism stimulation with bacterial LPS and modeling of MetS (LPS+MetS group).…”

“…The fourth group was the group of the combined На умовах ліцензії CC BY 4.0 effect of organism stimulation with bacterial LPS and modeling of MetS (LPS+MetS group). The animals of this group fed on a standard diet, received a 20% fructose solution as the only source of drinking water and an injection of bacterial LPS of S. typhi according to abovementioned scheme [8,9]. Animals were removed from the experiment under following procedure: 1.…”

“…The total concentration of glycosaminoglycans (GAG), the concentration of heparin-heparan, keratan-dermatan and chondroitin fractions of GAG, the concentration of free L-hydroxyproline and the concentration of sialic acids were determined spectrophotometrically in 10% rat heart homogenate [8]. All spectrophotometric studies were performed on spectrophotometer Ulab 101, on which we estimated the absorbance (A) of the sample on specified according to biochemical method wavelength (λ).…”

“…It was established that preconditioning of synovial cells using LPS leads to their formation of extracellular vesicles, which have an inhibitory effect on the degradation of ECM components in joints and are a potential therapeutic agent for the treatment of osteoarthritis [7]. Metabolic syndrome (MetS) and associated low-grade systemic inflammation can also affect ECM metabolism, as shown in our previous work [8].…”