“…(i) Treatment of animal or plant cell chromosomes in low ionic strength solutions causes nucleoprotein swelling only if the divalent metal ion concentration is very low (e.g., less than about 5 X 10~4 m MgClo). This has been reported in a wide variety of systems including Drosophila melanogaster salivary glands and mitotic and meiotic cells from grasshopper testis (Mazia, 1954), chicken erythrocytes (Davies and Spencer, 1962), Vicia faba root cells (Hyde, 1956), isolated metaphase chromosomes from mouse leukemia cells, Chinese hamster cells, and HeLa cells (Chorazy et al, 1963;Somers et al, 1963;J. A. Huberman, personal communication), and sea urchin sperms (Mazia, 1954;Solari, 1965).…”