1997
DOI: 10.1038/bjc.1997.506
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Examining the technique of angiogenesis assessment in invasive breast cancer

Abstract: Summary The intensity of angiogenesis as measured by the density of microvessels has been reported to be associated with a poor prognosis in invasive breast cancer in some, but not all, studies. The reasons for these discrepancies may be variations in the methodologies used. The monoclonal antibody used to identify the microvessels, the number of high-density areas or 'hotspots' counted and the type of value taken for statistical analysis (highest count or mean count) have varied between the different studies.… Show more

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Cited by 111 publications
(107 citation statements)
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References 31 publications
(44 reference statements)
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“…Both showed a significant increase in DCIS, with greater values in cases associated with invasive carcinoma. Our findings confirm those of Martin et al (1997), that CD34 antibodies give consistently higher vessel counts in breast carcinomas than antibodies against vWF or CD31.…”
Section: Mvd (Vessels MMsupporting
confidence: 90%
“…Both showed a significant increase in DCIS, with greater values in cases associated with invasive carcinoma. Our findings confirm those of Martin et al (1997), that CD34 antibodies give consistently higher vessel counts in breast carcinomas than antibodies against vWF or CD31.…”
Section: Mvd (Vessels MMsupporting
confidence: 90%
“…15 However, identification of vWF-producing osteosarcoma cells raises the possibility that other endothelial markers such as CD31 or CD34 may be more appropriate, especially for automated methods used to assess VD in tumors. 15,[36][37][38][39][40] Osteosarcomas with positive tumor cell staining tend to have high vWF mRNA expression (P ¼ 0.006), indicating that the osteosarcoma cells may contribute a significant proportion of the vWF mRNA expression seen in tumors. Furthermore, patients with paired tumor samples displayed higher vWF expression in the metastatic samples (P ¼ 0.005), suggesting that the metastatic process may select for tumor cells that express vWF more highly.…”
Section: Discussionmentioning
confidence: 99%
“…Anti-CD34 antibody seems to be more reliable in terms of specificity and reproducibility than monoclonal antibodies generated against other endothelial cell antigens (Tanigawa et al, 1996). In invasive breast cancer, CD34 has been shown to yield higher microvessel values than CD31 or factor VIII (Martin et al, 1997) and does not stain any tumour or inflammatory cells as CD31 or factor VIII. CD31 is a transmembrane glycoprotein highly expressed in mature and immature endothelium and its localisation at the endothelial cell junctions suggests an important role in transendothelial migration.…”
Section: Discussionmentioning
confidence: 99%
“…An international consensus on the methodology and criteria of evaluation of microvessel density proposed that anti-CD31 monoclonal antibody should be the standard for microvessel assessment (Vermeulen et al, 1996) as it is superior on paraffin sections. But as CD34 has been shown to yield higher microvessel values than CD31 or factor VIII in breast cancer (Martin et al, 1997), it might be useful to combine CD34 and CD31 antibodies. In lung cancer, Offersen et al (2001) compared the staining with these three antibodies.…”
Section: Discussionmentioning
confidence: 99%