The role of microvessel density on the survival of patients with lung cancer: a systematic review of the literature with meta-analysis

Meert, Anne-Pascale; Paesmans, Marianne; Martin, Benoı̂t; Delmotte, P.; Berghmans, Thierry; Verdebout, Jean-Marc; Lafitte, Jean-Jacques; Mascaux, Céline; Sculier, Jean-Paul

doi:10.1038/sj.bjc.6600551

Cited by 209 publications

(153 citation statements)

References 64 publications

Supporting

Mentioning

143

Contrasting

Unclassified

Order By: Relevance

“…Like other immunohistochemistry-based studies, quantitative evaluation of vascularity in tissue sections may be affected significantly by variations in methodologies including antibody selection, methods of antigen retrieval (AR), and methods of vessel density assessment (Vermeulen et al 1996, Meert et al 2002. We compared evaluation of neovasculature staining using anti-CD31 or anti-F VIII RAg antibodies in five different human cell lines grown as tumor xenografts and one mouse syngeneic breast cancer by using a panel of AR methods including high temperature AR with different buffered and enzymatic solutions.…”

Section: Abstract and Abbreviationsmentioning

confidence: 99%

“…Adjacent endothelial cell columns contact each other to form three-dimensional cords and loops that subsequently develop tubes with lumens. Angiogenesis is critical to tumor growth, neoplastic progression and metastasis (Meert, et al 2002). Immunohistochemical staining of microvessels to assess microvessel density (MVD) per unit area is associated with the degree of intratumor neovascularization, tumor metastatic capability and the prognosis for patients with many types of human solid cancers (Hlatky et al 2002).…”

Section: Abstract and Abbreviationsmentioning

confidence: 99%

See 1 more Smart Citation

Immunohistochemistry in the evaluation of neovascularization in tumor xenografts

Wang¹,

Stockard²,

Harkins

et al. 2008

Biotechnic & Histochemistry

140

View full text Add to dashboard Cite

Angiogenesis, or neovascularization, is known to play an important role in the neoplastic progression leading to metastasis. CD31 or Factor VIII-related antigen (F VIII RAg) immunohistochemistry (IHC), is widely used in experimental studies quantifing tumor neovascularization in immunocompromised animal models implanted with transformed human cell lines. Quantification, however, can be affected extensively by variations in the methodology used to measure vascularization including antibody selection, pretreatment with antigen retrieval and evaluation techniques. To examine this further, we examined the microvessel density and the intensity of microvascular staining among five different human tumor xenografts and a mouse syngeneic tumor using anti-CD31 and F VIII RAg IHC staining. Different antigen retrieval methods also were evaluated. Maximal retrieval of CD31 was achieved using 0.5 M Tris (pH 10) buffer, while maximum retrieval of F VIII RAg was achieved using 0.05% pepsin treatment of tissue sections. For each optimized retrieval condition, compared to F VIII RAg, anti-CD31 highlighted small vessels better. Furthermore, the microvessel density of CD31 was significantly greater than that of F VIII RAg decorated vessels (p < 0.001). The choice of antibody and antigen retrieval method has a significant affect on immunohistochemical findings when studying angiogenesis. One also must use caution when comparing studies in the literature that use different techniques and reagents.

show abstract

Section: Abstract and Abbreviationsmentioning

confidence: 99%

Section: Abstract and Abbreviationsmentioning

confidence: 99%

Immunohistochemistry in the evaluation of neovascularization in tumor xenografts

Wang¹,

Stockard²,

Harkins

et al. 2008

Biotechnic & Histochemistry

140

View full text Add to dashboard Cite

show abstract

“…By using the methodology of systematic reviews of the literature with meta-analyses, our Group has shown a prognostic role for several biological factors in lung cancer: p53 , Bcl-2 , vascular endothelial growth factor (VEGF) (Delmotte et al, 2002), epidermal growth factor receptor (EGFR) (Meert et al, 2002a), Neu and microvessel density (Meert et al, 2002b).…”

mentioning

confidence: 99%

Ki-67 expression and patients survival in lung cancer: systematic review of the literature with meta-analysis

et al. 2004

Self Cite

View full text Add to dashboard Cite

Among new biological markers that could become useful prognostic factors for lung carcinoma, Ki-67 is a nuclear protein involved in cell proliferation regulation. Some studies have suggested an association between Ki-67 and poor survival in lung cancer patients. In order to clarify this point, we have performed a systematic review of the literature, using the methodology already described by our Group, the European Lung Cancer Working Party. In total, 37 studies, including 3983 patients, were found to be eligible. In total, 49% of the patients were considered as having a tumour positive for the expression of Ki-67 according to the authors cutoff. In all, 29 of the studies dealt with non-small-cell lung carcinoma (NSCLC), one with small-cell carcinoma (SCLC), two with carcinoid tumours and five with any histology. In terms of survival results, Ki-67 was a bad prognosis factor for survival in 15 studies while it was not in 22. As there was no statistical difference in quality scores between the significant and nonsignificant studies evaluable for the meta-analysis, we were allowed to aggregate the survival results. The combined hazard ratio for NSCLC, calculated using a random-effects model was 1.56 (95% CI: 1.30 -1.87), showing a worse survival when Ki-67 expression is increased. In conclusion, our meta-analysis shows that the expression of Ki-67 is a factor of poor prognosis for survival in NSCLC.

show abstract

“…In order to clarify the prognostic impact of other biological factors in lung cancer, our group has performed systematic reviews of the literature with meta-analyses. It allowed us to show that VEGF (Delmotte et al, 2002), microvessel density (Meert et al, 2002b), EGFR (Meert et al, 2002a), HER-2/Neu , Ki-67 (Martin et al, 2004), K-Ras (Mascaux et al, 2005a) and p53 (Steels et al, 2001) have a negative impact on survival, whereas Bcl-2 is associated with a favourable survival effect, at least when studying their impact in univariate analysis.…”

mentioning

confidence: 99%

Has Cox-2 a prognostic role in non-small-cell lung cancer? A systematic review of the literature with meta-analysis of the survival results

et al. 2006

Self Cite

View full text Add to dashboard Cite

Cyclooxygenase-2 (COX-2) is overexpressed in lung cancer, especially in adenocarcinoma (ADC). Our aim was to determine the prognostic value of COX-2 on survival in patients with lung cancer. Studies evaluating the survival impact of COX-2 in lung cancer, published until December 2005, were selected. Data for estimation of individual hazard ratios (HR) for survival were extracted from the publications and combined in a pooled HR. Among 14 eligible papers, all dealing with non-small-cell lung cancer, 10 provided results for meta-analysis of survival data (evaluable studies). Cyclooxygenase-2 positivity was associated with reduced survival, improved survival or no statistically significant impact in six, one and seven studies, respectively. Combined HR for the 10 evaluable studies (1236 patients) was 1.39 (95% confidence intervals (CI): 0.97 -1.99). In stage I lung cancer (six evaluable studies, 554 patients), it was 1.64 (95% CI: 1.21 -2.24). No significant impact was shown in ADC. A slight detrimental effect on survival in patients with lung cancer is associated with COX-2 expression, but the statistical significance is not reached. This effect is statistically significant in stage I, suggesting that COX-2 expression could be useful at early stages to distinguish those with a worse prognosis.

show abstract

The role of microvessel density on the survival of patients with lung cancer: a systematic review of the literature with meta-analysis

Cited by 209 publications

References 64 publications

Immunohistochemistry in the evaluation of neovascularization in tumor xenografts

Immunohistochemistry in the evaluation of neovascularization in tumor xenografts

Ki-67 expression and patients survival in lung cancer: systematic review of the literature with meta-analysis

Has Cox-2 a prognostic role in non-small-cell lung cancer? A systematic review of the literature with meta-analysis of the survival results

Contact Info

Product

Resources

About