Examination of synovial fluid hyaluronan quantity and quality in stifle joints of dogs with osteoarthritis

Venable, Rachel O.; Stoker, Aaron M.; Cook, Cristi R.; Cockrell, Mary; Cook, James L.

doi:10.2460/ajvr.69.12.1569

Cited by 27 publications

(18 citation statements)

References 14 publications

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“…As reported by Venable et al [ 28 ], HA may be associated with cartilage damage in early pathologic changes of OA. The treatment of OA by intra-articular HA injection has been commonly used to promote cartilage repair, and the mechanisms contributing to this function are proposed to include the suppression of proinflammatory cytokines and chemokines, the promotion of the anabolism, and the relief of pain [ 29 , 30 ].…”

Section: Discussionmentioning

confidence: 60%

Human Urine-Derived Stem Cells: Potential for Cell-Based Therapy of Cartilage Defects

Chen

Xing

et al. 2018

Stem Cells International

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Stem cell therapy is considered an optimistic approach to replace current treatments for cartilage defects. Recently, human urine-derived stem cells (hUSCs), which are isolated from the urine, are studied as a promising candidate for many tissue engineering therapies due to their multipotency and sufficient proliferation activities. However, it has not yet been reported whether hUSCs can be employed in cartilage defects. In this study, we revealed that induced hUSCs expressed chondrogenic-related proteins, including aggrecan and collagen II, and their gene expression levels were upregulated in vitro. Moreover, we combined hUSCs with hyaluronic acid (HA) and injected hUSCs-HA into a rabbit knee joint with cartilage defect. Twelve weeks after the injection, the histologic analyses (HE, toluidine blue, and Masson trichrome staining), immunohistochemistry (aggrecan and collagen II), and histologic grade of the sample indicated that hUSCs-HA could stimulate much more neocartilage formation compared with hUSCs alone, pure HA, and saline, which only induced the modest cartilage regeneration. In this study, we demonstrated that hUSCs could be a potential cell source for stem cell therapies to treat cartilage-related defects in the future.

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Section: Discussionmentioning

confidence: 60%

Human Urine-Derived Stem Cells: Potential for Cell-Based Therapy of Cartilage Defects

Chen

Xing

et al. 2018

Stem Cells International

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“…The quantity and quality of HA in synovial fluid are changed in dogs with OA and HA may be associated with early pathological changes in cartilage damage 41 . Intra-articular HA injection for the treatment of cartilage damage has been commonly used, but the efficacy is variable.…”

Section: Discussionmentioning

confidence: 99%

Mesenchymal Stem Cells in Combination with Hyaluronic Acid for Articular Cartilage Defects

Duan

Fan

et al. 2018

Sci Rep

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Mesenchymal stem cells (MSCs) and hyaluronic acid (HA) have been found in previous studies to have great potential for medical use. This study aimed to investigate the therapeutic effects of bone marrow mesenchymal stem cells (BMSCs) combined with HA on articular cartilage repair in canines. Twenty-four healthy canines (48 knee-joints), male or female with weight ranging from 5 to 6 kg, were operated on to induce cartilage defect model and divided into 3 groups randomly which received different treatments: BMSCs plus HA (BMSCs-HA), HA alone, and saline. Twenty-eight weeks after treatment, all canines were sacrificed and analyzed by gross appearance, magnetic resonance imaging (MRI), hematoxylin-eosin (HE) staining, Masson staining, toluidine blue staining, type II collagen immunohistochemistry, gross grading scale and histological scores. MSCs plus HA regenerated more cartilage-like tissue than did HA alone or saline. According to the macroscopic evaluation and histological assessment score, treatment with MSCs plus HA also lead to significant improvement in cartilage defects compared to those in the other 2 treatment groups (P < 0.05). These findings suggested that allogeneic BMSCs plus HA rather than HA alone was effective in promoting the formation of cartilage-like tissue for repairing cartilage defect in canines.

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“…Importantly, efficiency of HA-based treatments is decreased by ROS produced in damaged knees ( 149 ), underscoring the importance of oxidative stress in knee pathology. Fragmented HA has been found in OA knee joints both in human and canine patients and is correlated with lower load-bearing capacity ( 150 , 151 ). It is likely that oxidative stress caused by meniscal pathology also negatively influences HA in dogs.…”

Section: Biological Hallmarks Of Meniscal Pathology In Human and Dogmentioning

confidence: 99%

The Pathobiology of the Meniscus: A Comparison Between the Human and Dog

Krupková¹,

Smolders²,

Wuertz‐Kozak

et al. 2018

Front. Vet. Sci.

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Serious knee pain and related disability have an annual prevalence of approximately 25% on those over the age of 55 years. As curative treatments for the common knee problems are not available to date, knee pathologies typically progress and often lead to osteoarthritis (OA). While the roles that the meniscus plays in knee biomechanics are well characterized, biological mechanisms underlying meniscus pathophysiology and roles in knee pain and OA progression are not fully clear. Experimental treatments for knee disorders that are successful in animal models often produce unsatisfactory results in humans due to species differences or the inability to fully replicate disease progression in experimental animals. The use of animals with spontaneous knee pathologies, such as dogs, can significantly help addressing this issue. As microscopic and macroscopic anatomy of the canine and human menisci are similar, spontaneous meniscal pathologies in canine patients are thought to be highly relevant for translational medicine. However, it is not clear whether the biomolecular mechanisms of pain, degradation of extracellular matrix, and inflammatory responses are species dependent. The aims of this review are (1) to provide an overview of the anatomy, physiology, and pathology of the human and canine meniscus, (2) to compare the known signaling pathways involved in spontaneous meniscus pathology between both species, and (3) to assess the relevance of dogs with spontaneous meniscal pathology as a translational model. Understanding these mechanisms in human and canine meniscus can help to advance diagnostic and therapeutic strategies for painful knee disorders and improve clinical decision making.

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Examination of synovial fluid hyaluronan quantity and quality in stifle joints of dogs with osteoarthritis

Abstract: Synovial fluid hyaluronan quantity and quality were altered in stifle joints of dogs with osteoarthritis, compared with control stifle joints. A specific hyaluronan protein fragment may be associated with early pathologic changes in affected joints.

Cited by 27 publications

References 14 publications

Human Urine-Derived Stem Cells: Potential for Cell-Based Therapy of Cartilage Defects

Human Urine-Derived Stem Cells: Potential for Cell-Based Therapy of Cartilage Defects

Mesenchymal Stem Cells in Combination with Hyaluronic Acid for Articular Cartilage Defects

The Pathobiology of the Meniscus: A Comparison Between the Human and Dog

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