Exacerbation of experimental colitis by nonsteroidal anti-inflammatory drugs is not related to elevated leukotriene B4 synthesis

Wallace, John L.; Keenan, Catherine M.; Gale, Donna D; Shoupe, T. Scott

doi:10.1016/0016-5085(92)91779-4

Cited by 144 publications

(86 citation statements)

References 30 publications

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“…In the current study, the exacerbation in the above mentioned NSAIDs was shown by decreased hemoglobin and plasma albumin concentration, macroscopic and histological higher colonic damage, and a high mortality incidence. These data are in agreement with very similar studies by Wallace et al (53) and Reuter et al (46) . In the meantime, ketoprofen presented a lower inflammatory activity than indometacin and diclofenac measured by the aforementioned parameters, and a high survival rate (88%), whereas celecoxib showed the lowest inflammatory exacerbation between the different NSAIDs with 100% of survival rate.…”

Section: Discussionsupporting

confidence: 94%

“…Exacerbation of inflammatory response in ulcerative colitis by nonsteroidal anti-inflammatory drugs in general, and specifically by indometacin and diclofenac, has been verified by several authors in case reports (10,51) or in experimental colitis (46,53) . In the current study, the exacerbation in the above mentioned NSAIDs was shown by decreased hemoglobin and plasma albumin concentration, macroscopic and histological higher colonic damage, and a high mortality incidence.…”

Section: Discussionmentioning

confidence: 86%

“…Treatment with different NSAIDs began 3h before induction of colitis and continued every 12h thereafter for up to 5d. The doses were selected because it has been shown to exert significant antiinflammatory effects in rats (6,38,46,53,55) . The others groups were treated orally with 0.5% carboxymethylcellose with the same periodicity.…”

Section: Animal Groups and Treatmentmentioning

confidence: 99%

“…Then, the segment was placed on a flat glass plate with the mucosal surface up. The degree of damage was scored macroscopically based on the presence of adhesions, strictures, ulcers, and wall thickness by an observer blinded to the treatment the rats had received (53) . Soon after, in order to study the oxidative stress, the mucosa was scraped off with a microscope slide onto a cold Petri dish and all mucosa specimens were frozen in liquid nitrogen.…”

Section: Evaluation Of Colon Damage and Sample Preparationmentioning

confidence: 99%

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COMPARISON OF SELECTIVE AND NON SELECTIVE CYCLO-OXYGENASE 2 INHIBITORS IN EXPERIMENTAL COLITIS EXACERBATION: role of leukotriene B4 and superoxide dismutase

Breganó

Barbosa

Kadri

et al. 2014

Arq. Gastroenterol.

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-Context -Nonsteroidal anti-inflammatory drugs are considered one of the most important causes of reactivation of inflammatory bowel disease. With regard to selective cyclo-oxygenase 2 inhibitors, the results are controversial in experimental colitis as well as in human studies. Objective -The aim this study is to compare nonsteroidal anti-inflammatory drugs effects, selective and non selective cyclo-oxygenase 2 inhibitors, in experimental colitis and contribute to the understanding of the mechanisms which nonsteroidal anti-inflammatory drugs provoke colitis exacerbation. Methods -Six groups of rats: without colitis, with colitis, and colitis treated with celecoxib, ketoprofen, indometacin or diclofenac. Survival rates, hemoglobin, plasmatic albumin, colonic tissue of interleukin-1ß, interleukin-6, tumor necrosis factor alpha, prostaglandin E2, catalase, superoxide dismutase, thiobarbituric acid-reactive substances, chemiluminescence induced by tert-butil hydroperoxides, and tissue and plasmatic leukotriene B4 were determined. Results -The groups treated with diclofenac or indometacin presented lower survival rates, hemoglobin and albumin, higher tissue and plasmatic leukotriene B4 and tissue superoxide dismutase than the group treated with celecoxib. Ketoprofen presented an intermediary behavior between diclofenac/indometacin and celecoxib, concerning to survival rate and albumin. The groups without colitis, with colitis and with colitis treated with celecoxib showed leukotriene B4 and superoxide dismutase lower levels than the groups treated with nonselective cyclo-oxygenase 2 inhibitors. Conclusions -Diclofenac and indometacin presented the highest degree of induced colitis exacerbation with nonsteroidal anti-inflammatory drugs, celecoxib did not show colitis exacerbation, and ketoprofen presented an intermediary behavior between diclofenac/indometacin and celecoxib. These results suggest that leukotriene B4 and superoxide dismutase can be involved in the exacerbation of experimental colitis by nonselective nonsteroidal anti-inflammatory drugs. HEADINGS -Colitis. Non-steroidal anti-inflammatory agents. Eicosanoids. Oxidative stress.

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Section: Discussionsupporting

confidence: 94%

Section: Discussionmentioning

confidence: 86%

Section: Animal Groups and Treatmentmentioning

confidence: 99%

Section: Evaluation Of Colon Damage and Sample Preparationmentioning

confidence: 99%

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COMPARISON OF SELECTIVE AND NON SELECTIVE CYCLO-OXYGENASE 2 INHIBITORS IN EXPERIMENTAL COLITIS EXACERBATION: role of leukotriene B4 and superoxide dismutase

Breganó

Barbosa

Kadri

et al. 2014

Arq. Gastroenterol.

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“…Rats were anesthetized with administration of 45 mg/kg pentobarbital sodium intraperitoneally [38], which was repeated if necessary. At first, a mixture of 30 mg TNBS in 0.25 ml of ethanol 50% was used intrarectally for induction of colitis [39]. Due to a high rate of mortality, the method was modified to the following mode, in which macroscopic and microscopic evidence showed a proper induction of colitis: the rats were positioned on their right side and 0.3 ml of a mixture containing 6 volume of 5% TNBS plus 4 volume of 99% ethanol was instilled intracolonically (10 mg TNBS per rat) using a rubber cannula (8 cm long).…”

Section: Induction Of Colitismentioning

confidence: 99%

On the benefits of silymarin in murine colitis by improving balance of destructive cytokines and reduction of toxic stress in the bowel cells

et al. 2009

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Inflammatory bowel disease (IBD) is a multifactorial disease with an unknown etiology characterized by oxidative stress, leukocyte infiltration and a rise in inflammatory cytokines. In this study, we have investigated the effects of silymarin, a mixture of several flavonolignans with established antioxidant and anti-inflammatory properties, on trinitrobenzene sulphonic acid (TNBS)-induced colitis in rats. Experimental colitis was induced in male Wistar-albino rats by delivering TNBS to the distal colon. All the medicines were administered by gavage for seven days. Thirty-six male rats were divided into six groups containing six rats in each one. Control rats received only TNBS. In the treated groups, animals were given different doses of silymarin (40, 80, and 160 mg/kg). Dexamethasone (1 mg/kg) was used as the positive treatment. Colonic status was investigated seven days post induction of colitis through macroscopic, histological, and biochemical analyses. Amelioration of the morphological signs including macroscopic damage, necrotic area, and histology were seen subsequent to treating animals with silymarin. These observations were accompanied by a significant reduction in the degree of both neutrophil infiltration, indicated by decreased myeloperoxidase activity, and lipid peroxidation, as measured by a decline in malodialdehyde content in inflamed colon as well as a decrease in levels of inflammatory cytokines (TNF-α and IL-1β). The results of the present study reveal that the beneficial effect of silymarin in bowel cells is mediated through its anti-oxidant and anti-inflammatory potentials.

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Unresolved Issues in the Role of Cyclooxygenase-2 in Normal Physiologic Processes and Disease

Lipsky¹,

Brooks²,

Crofford³

et al. 2000

Arch Intern Med

141

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Originally suggested to function mainly in inflammatory situations, recent data have implied important roles for the cyclooxygenase-2 isoenzyme in reproductive biologic processes, renal and neurologic function, and the antithrombotic activities of endothelial cells. As cyclooxygenase-2-specific inhibitors have recently become available as analgesic and anti-inflammatory drugs, a comprehensive view of this rapidly evolving field is necessary to anticipate both the potential therapeutic benefits and toxic effects associated with these agents.

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Exacerbation of experimental colitis by nonsteroidal anti-inflammatory drugs is not related to elevated leukotriene B4 synthesis

Cited by 144 publications

References 30 publications

COMPARISON OF SELECTIVE AND NON SELECTIVE CYCLO-OXYGENASE 2 INHIBITORS IN EXPERIMENTAL COLITIS EXACERBATION: role of leukotriene B4 and superoxide dismutase

COMPARISON OF SELECTIVE AND NON SELECTIVE CYCLO-OXYGENASE 2 INHIBITORS IN EXPERIMENTAL COLITIS EXACERBATION: role of leukotriene B4 and superoxide dismutase

On the benefits of silymarin in murine colitis by improving balance of destructive cytokines and reduction of toxic stress in the bowel cells

Unresolved Issues in the Role of Cyclooxygenase-2 in Normal Physiologic Processes and Disease

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