Ex vivo studies of polymorphonuclear neutrophils from patients with early‐onset periodontitis

Abstract

In this study, we assessed the LFA‐1 (CD18/CD11a) and CR3 (CD18/CD11b) expression on peripheral polymorphonuclear leukocytes (PB‐PMN) and crevicular fluid polymorphonuclear leukocytes (CF‐PMN), by subjects with a healthy periodontium (n=1), gingivitis (n=8). early‐onset periodontitis (n=17) and adult periodontitis (n=8). Using flow cytometry analysis, the %s of CD18, CD11a and CD11b positive cells and the absolute numbers of fluorescent molecules were determined. No significant difference could be found among the 4 groups, for these 2 kinds of parameters, in PB‐PMN or CF‐PMN. However, a great difference could be noted between the results obtained from PB‐PMN and those obtained from CF‐PMN. The %s of positive CF‐PMN were significantly lower than those of PB‐PMN for the 3 sub‐units (p < 0.001). The levels of CD18 and CD11b expressed by CF‐PMN were higher than those expressed by PB‐PMN and the difference was significant for CD11b (p < 0.001). On the contrary, the level of CD11a expressed on CF‐PMN was significantly lower than that expressed by PB‐PMN (p < 0.001). Hence, our current results show that early‐onset periodontitis PMN can be quite normal and this fact is not surprising insofar as, in our study, these cells were perfectly functional and all the subjects were in good health. We concluded that the analysis of the leukocyte adhesion receptors expression on PB‐PMN does not appear useful for helping to establish a differential diagnosis between the different forms of periodontitis. However, the pattern of expression study on CF‐PMN may permit a better comprehension of the local phenomena which are implicated in the defence of the periodontal tissues against oral microorganisms.

The effects of different (pseudo)halide substrates on peroxidase‐mediated killing of Actinobacillus actinomycetemcomitans

“…Studies of the functional capability of peripheral PMNs have, however, given controversial results, in particular between American and Scandinavian LJP patients (45,46). Data from patients with rapidly progressing earlyonset periodontitis show that PMNs isolated from gingival crevicular fluid at diseased sites have a decreased functional capability when compared to those from healthy sites or from peripheral blood from the same patients, suggesting a local rather than intrinsic abnormality (47,48). The clinical significance of functionally "disabled" PMNs remains to be evaluated, since metabolically active PMN cells are the main source of H,O, in the subgingival region.…”

Peroxidases, Iactoferrin and lysozyme in peripheral blood neutrophils, gingival crevicular fluid and whole saliva of patients with localized juvenile periodontitis

“…Gingival crevicular fluid (GCF) analyses in adult periodontitis (AP) patients have indicated that the local inflammatory reaction in the gingiva is host-and not site-specific, ie the tissue destruction reflects a specific host response (Gustafsson et al, 1995). On the other hand, data from patients with rapidly progressing early-onset periodontitis show that PMNs isolated from GCF at diseased sites have a decreased functional capability when compared to those from healthy sites or from peripheral blood from the same patients, suggesting a local rather than intrinsic abnormality (Murray and Patters, 1980;Mouynet et al, 1995).…”