Ex‐CARS: exotic configuration for coherent anti‐Stokes Raman scattering microspectroscopy utilizing two laser sources

Yakovlev, Vladislav V.; Petrov, Georgi I.; Noojin, Gary D.; Harbert, Corey A.; Denton, Michael L.; Thomas, Robert J.

doi:10.1002/jbio.201000042

Cited by 3 publications

(3 citation statements)

References 21 publications

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“…While our current cwSRS system is able to capture high-dynamic range images, several follow up studies may be required to either to further characterize cwSRS microscopy or to improve this imaging modality. The same concept can be also extended for stimulated Raman photoacoustic imaging [21, 30]. …”

Section: Discussionmentioning

confidence: 99%

Continuous-wave stimulated Raman scattering (cwSRS) microscopy

2013

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Stimulated Raman scattering (SRS) microscopy is a powerful tool for chemically-sensitive non-invasive optical imaging. However, ultrafast laser sources, which are currently employed, are still expensive and require substantial maintenance to provide temporal overlap and spectral tuning. SRS imaging, which utilizes continuous-wave laser sources, has a major advantage, as it eliminates the cell damage due to exposure to the high-intensity light radiation, while substantially reducing the cost and complexity of the set-up. As a proof-of-principle, we demonstrate microscopic imaging of dimethyl sulfoxide using two independent, commonly used lasers, a diode-pumped, intracavity doubled 532-nm laser and a He-Ne laser operating at 632.8-nm

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Section: Discussionmentioning

confidence: 99%

Continuous-wave stimulated Raman scattering (cwSRS) microscopy

2013

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“…However, only microscopic imaging, which is demonstrated in this report, can fully realize the great potential of this approach. A somewhat similar approach has been recently implemented in our group for CARS microscopy, when we combined a cw laser with a 10-fs Ti:sapphire oscillator for a broadband CARS imaging [37]. …”

Section: Introductionmentioning

confidence: 91%

Stimulated Raman microscopy without ultrafast lasers

2013

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Stimulated Raman scattering (SRS) microscopy is a powerful tool for chemically-sensitive non-invasive optical imaging. However, the short-pulse laser sources, which are currently being employed for this imaging technique, are still expensive and require substantial maintenance to provide temporal and spectral overlap. SRS imaging, which utilizes cw laser sources, has a major advantage over pulsed lasers, as it eliminates the possibility of cell damage due to exposure to high-intensity light radiation, while substantially reducing the cost and complexity of the set-up and keeping a subcellular spatial resolution. As a proof-of-principle, we demonstrate microscopic imaging of dimethyl sulfoxide using two independent, commonly used and inexpensive lasers: a diode-pumped, intracavity doubled 532 nm laser and a He-Ne laser operating at 633 nm. In our proof-of-principle experience, dimethyl sulfoxide acts as a contrast agent providing Raman scattering signal. The 532 nm and 633 nm lasers act as excitation and probe sources, respectively [1].

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“…In a spectroscopic application, a broadband few cycle Ti:Sa-laser was used to illuminate the sample, while a 532 nm cw laser was used to detect spontaneous and coherent Raman signals. 104 The overall CARS signal yield was 2% of the total signal. To increase the efficiency of CARS signal generation, higher repetition rate lasers can be used.…”

Section: Instrumentation For Coherent Raman Scatteringmentioning

confidence: 98%

Developments in spontaneous and coherent Raman scattering microscopic imaging for biomedical applications

et al. 2016

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First, the potential role of Raman-based techniques in biomedicine is introduced. Second, an overview about the instrumentation for spontaneous and coherent Raman scattering microscopic imaging is given with a focus of recent developments. Third, imaging strategies are summarized including sequential registration with laser scanning microscopes, line imaging and global or wide-field imaging. Finally, examples of biomedical applications are presented in the context of single cells, laser tweezers, tissue sections, biopsies and whole animals.

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Ex‐CARS: exotic configuration for coherent anti‐Stokes Raman scattering microspectroscopy utilizing two laser sources

Cited by 3 publications

References 21 publications

Continuous-wave stimulated Raman scattering (cwSRS) microscopy

Continuous-wave stimulated Raman scattering (cwSRS) microscopy

Stimulated Raman microscopy without ultrafast lasers

Developments in spontaneous and coherent Raman scattering microscopic imaging for biomedical applications

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